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Glycoconjugate Journal

, Volume 26, Issue 3, pp 335–347 | Cite as

Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or LeY in the preimplantation mouse embryo

  • Suzannah A. Williams
  • Pamela Stanley
Article

Abstract

The glycan epitopes termed stage-specific embryonic antigens (SSEA) occur on glycoproteins and glycolipids in mammals. However, it is not known whether these epitopes are attached to N- or O-glycans on glycoproteins and/or on glycolipids in the developing mouse embryo. In this paper the expression of the antigens SSEA-1, SSEA-3, SSEA-4 and LeY was examined on ovulated eggs, early embryos and blastocysts lacking either complex and hybrid N-glycans or core-1 derived O-glycans. In all cases, antigen expression determined by fluorescence microscopy of bound monoclonal antibodies to embryos at the stage of development of maximal expression was similar in mutant and control embryos. Thus, none of these developmental antigens are expressed solely on either complex N- or core 1-derived O-glycans attached to glycoproteins in the preimplantation mouse embryo. Furthermore, neither of these classes of glycan is essential for the expression of SSEA-1, SSEA-3, SSEA-4 or LeY on mouse embryos.

Keywords

N-glycans O-glycans Mgat1 T-synthase Preimplantation mouse embryos SSEA Immunofluorescence microscopy 

Abbreviations

SSEA

stage-specific embryonic antigen

LeX

Lewis X

LeY

Lewis Y

L-PHA

leukoagglutinin from P. vulgaris

PNA

peanut agglutinin

MAb

monoclonal antibody

FITC

fluorescein isothiocyanate

TRITC

tetramethylrhodamine isothiocyanate

Notes

Acknowledgements

We thank Wen Dong for excellent technical assistance and Dr. Jason Aglipay for help with some experiments. This work was supported by grant RO1 30645 from the National Institutes of Health to P. S. and received partial support from the Albert Einstein Cancer Center grant PO1 13330.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of Cell BiologyAlbert Einstein College of MedicineNew YorkUSA
  2. 2.Department of Physiology, Anatomy and GeneticsUniversity of OxfordOxfordUK

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