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Glycoconjugate Journal

, Volume 24, Issue 8, pp 421–428 | Cite as

Isolation and structural determination of a unique polysaccharide containing mannofuranose from the cell wall of the fungus Acrospermum compressum

  • Alicia Prieto
  • Juan Antonio Leal
  • María Inmaculada Giménez-Abián
  • Ángeles Canales
  • Jesús Jiménez-Barbero
  • Manuel Bernabé
Article

Abstract

The alkali-extractable and water-soluble fungal polysaccharide F1SS isolated from the cell wall of Acrospermum compressum has been studied by methylation analyses, reductive cleavage and 1D- and 2D-NMR spectroscopy. The polysaccharide consists of a regular disaccharide repeating unit with the structure: The mannan core was obtained by mild hydrolysis of the polysaccharide F1SS and its structure was deduced to be composed of a skeleton of α-(1→6)-mannopyranan, with around 1 out of 11 residues substituted at position 2 by short chains (one to six units) of 2-substituted mannopyranoses. DOSY experiments provided molecular sizes of 60 kDa and 2.5 kDa for the polysaccharide F1SS and the mannan core, respectively. This is the first report of a fungal mannofuranose-containing cell wall polysaccharide.

Keywords

Cell wall polysaccharide Acrospermaceae Fungi NMR spectroscopy mannofuranose 

Abbreviations

CBS

Centraalbureau voor Schimmelcultures

DEPT

distortionless enhancement by polarization transfer

DOSY

diffusion ordered NMR spectroscopy

DQF-COSY

double quantum filtered correlated spectroscopy

F1SS

alkali extractable and water soluble polysaccharide

GC

gas chromatography

GC-MS

gas chromatography-mass spectrometry

HMBC

heteronuclear multiple bond correlation

HMQC

heteronuclear multiple quantum coherence

NOESY

nuclear Overhauser enhancement spectroscopy

TFA

trifluoroacetic acid

TMSi

trimethylsilyl

TOCSY

totally correlated spectroscopy

Notes

Acknowledgments

The authors thank Mr. J. López for technical assistance. This work was supported by Grant MEC CTQ-2006-10874-C02-01 from Dirección General de Investigación Científica y Técnica.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Alicia Prieto
    • 1
  • Juan Antonio Leal
    • 1
  • María Inmaculada Giménez-Abián
    • 1
  • Ángeles Canales
    • 1
  • Jesús Jiménez-Barbero
    • 1
  • Manuel Bernabé
    • 2
  1. 1.Centro de Investigaciones Biológicas. CSICCiudad UniversitariaMadridSpain
  2. 2.Departamento de Química Orgánica BiológicaInstituto de Química Orgánica. CSICMadridSpain

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