Glycoconjugate Journal

, Volume 24, Issue 8, pp 421–428 | Cite as

Isolation and structural determination of a unique polysaccharide containing mannofuranose from the cell wall of the fungus Acrospermum compressum

  • Alicia Prieto
  • Juan Antonio Leal
  • María Inmaculada Giménez-Abián
  • Ángeles Canales
  • Jesús Jiménez-Barbero
  • Manuel Bernabé


The alkali-extractable and water-soluble fungal polysaccharide F1SS isolated from the cell wall of Acrospermum compressum has been studied by methylation analyses, reductive cleavage and 1D- and 2D-NMR spectroscopy. The polysaccharide consists of a regular disaccharide repeating unit with the structure: The mannan core was obtained by mild hydrolysis of the polysaccharide F1SS and its structure was deduced to be composed of a skeleton of α-(1→6)-mannopyranan, with around 1 out of 11 residues substituted at position 2 by short chains (one to six units) of 2-substituted mannopyranoses. DOSY experiments provided molecular sizes of 60 kDa and 2.5 kDa for the polysaccharide F1SS and the mannan core, respectively. This is the first report of a fungal mannofuranose-containing cell wall polysaccharide.


Cell wall polysaccharide Acrospermaceae Fungi NMR spectroscopy mannofuranose 



Centraalbureau voor Schimmelcultures


distortionless enhancement by polarization transfer


diffusion ordered NMR spectroscopy


double quantum filtered correlated spectroscopy


alkali extractable and water soluble polysaccharide


gas chromatography


gas chromatography-mass spectrometry


heteronuclear multiple bond correlation


heteronuclear multiple quantum coherence


nuclear Overhauser enhancement spectroscopy


trifluoroacetic acid




totally correlated spectroscopy



The authors thank Mr. J. López for technical assistance. This work was supported by Grant MEC CTQ-2006-10874-C02-01 from Dirección General de Investigación Científica y Técnica.


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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Alicia Prieto
    • 1
  • Juan Antonio Leal
    • 1
  • María Inmaculada Giménez-Abián
    • 1
  • Ángeles Canales
    • 1
  • Jesús Jiménez-Barbero
    • 1
  • Manuel Bernabé
    • 2
  1. 1.Centro de Investigaciones Biológicas. CSICCiudad UniversitariaMadridSpain
  2. 2.Departamento de Química Orgánica BiológicaInstituto de Química Orgánica. CSICMadridSpain

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