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Glycoconjugate Journal

, Volume 23, Issue 3–4, pp 175–187 | Cite as

Apoptosis of human breast carcinoma cells in the presence of cis-platin and L-/D-PPMP: IV. Modulation of replication complexes and glycolipid: Glycosyltransferases

  • Patrick J. Boyle
  • Rui Ma
  • Narendra Tuteja
  • Sipra Banerjee
  • Subhash Basu
Article

Abstract

Apoptosis of human breast carcinoma cells (SKBR-3, MCF-7, and MDA-468) has been observed after treatment of these cells with anti-cancer drug cis-platin and glycosphingolipid biosynthesis inhibitor L- and D-PPMP, respectively. These drugs initiated apoptosis in a dose-dependent manner as measured by phenotypic morphological changes, by binding of a fluorescent phophatidyl serine-specific dye (PSS-380) onto the outer leaflet of the cell membranes, and by activation of caspases, −3, −8, and −9. It was observed that in two hours very little apoptotic process had started but predominant biochemical changes occurred after 6 h. DNA degradation started after 24 hours of drug treatment. However, very little is known about the stability of the ‘`Replication Complexes’’ during the apoptotic process. DNA helicases are motor proteins that catalyze the melting of genomic DNA during its replication, repair, and recombination processes. Previously, DNA helicase-III was characterized as a component of the replication complexes isolated from embryonic chicken brains as well as breast and colon carcinoma cells. Helicase activities were measured by a novel method (ROME assay), and DNA polymerase-α activities were determined by regular chain extension of the nicked ACT-DNA, by determining values obtained from +/− aphidicolin-treated incubation mixtures. In all three breast carcinoma cell lines, a common trend was observed: a decrease of activities of DNA polymerase-α and Helicase III. A sharp decrease of activities of the glycolipid sialyltransferases: SAT-2 (CMP-NeuAc; GD3 α2-8 sialyltransferase) and SAT-4 (CMP-NeuAc: GM1a α2-3 sialyltransferase) was observed in the apoptotic carcinoma cells treated with L-PPMP compared with cis-platin.

Keywords

Apoptosis Anti-cancer drugs Carcinoma cells cis-platin Caspase-3 Caspase-9 DNA polymerase-α Helicase-III GD3 ganglioside GD1a ganglioside D-PPMP L-PPMP SAT-2 SAT-3 SAT-4 

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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Patrick J. Boyle
    • 1
  • Rui Ma
    • 1
  • Narendra Tuteja
    • 2
  • Sipra Banerjee
    • 3
  • Subhash Basu
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of Notre DameNotre DameUSA
  2. 2.Plant and Molecular BiologyInternational Center for Genetic Engineering & Biotechnology (ICGEB)New DelhiIndia
  3. 3.Department of Cancer BiologyCleveland Clinic FoundationClevelandUSA

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