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Glycoconjugate Journal

, Volume 22, Issue 1–2, pp 21–26 | Cite as

Expression of complex-type N-glycans in developmental periods of zebrafish embryo

  • Tatsuya Takemoto
  • Shunji Natsuka
  • Shin-ichi Nakakita
  • Sumihiro Hase
Article

Abstract

As a first step to elucidate a role of N-glycans in development of vertebrates, we analyzed structures of the glycans expressed in early stages of zebrafish embryo. N-glycans were prepared from zebrafish embryos at several developmental stages followed by tagging with a fluorophore, 2-aminopyridine. The labeled glycans were analyzed by two modes of HPLC’s. The comparison of the elution profiles of HPLC’s unveil the change of the oligosaccharide structure during the development. These peaks were merely detected during 4–7 h after fertilization, however, increased from 12 h, and at 15 h a fairly amount of them was appeared. Structure analysis revealed that they were bianntenary complex-type N-glycans with or without fucose and/or bisecting N-acetylglucosamine residues. These results suggest that the complex-type N-glycans are concerned in some developmental event from segmentation period downward in zebrafish. Published in 2005.

Keywords

N-glycans zebrafish embryogenesis pyridylamination 

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Tatsuya Takemoto
    • 1
  • Shunji Natsuka
    • 1
  • Shin-ichi Nakakita
    • 2
  • Sumihiro Hase
    • 1
    • 3
  1. 1.Department of Chemistry, Graduate School of ScienceOsaka UniversityToyonaka, OsakaJapan
  2. 2.Department of Functional Glycomics, Life Science Research CenterKagawa UniversityJapan
  3. 3.Department of Chemistry, Graduate School of ScienceOsaka UniversityToyonaka, OsakaJapan

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