Expression of complex-type N-glycans in developmental periods of zebrafish embryo
- 126 Downloads
As a first step to elucidate a role of N-glycans in development of vertebrates, we analyzed structures of the glycans expressed in early stages of zebrafish embryo. N-glycans were prepared from zebrafish embryos at several developmental stages followed by tagging with a fluorophore, 2-aminopyridine. The labeled glycans were analyzed by two modes of HPLC’s. The comparison of the elution profiles of HPLC’s unveil the change of the oligosaccharide structure during the development. These peaks were merely detected during 4–7 h after fertilization, however, increased from 12 h, and at 15 h a fairly amount of them was appeared. Structure analysis revealed that they were bianntenary complex-type N-glycans with or without fucose and/or bisecting N-acetylglucosamine residues. These results suggest that the complex-type N-glycans are concerned in some developmental event from segmentation period downward in zebrafish. Published in 2005.
KeywordsN-glycans zebrafish embryogenesis pyridylamination
Unable to display preview. Download preview PDF.
- 1.Wacker M, Linton D, Hitchen PG, Nita-Lazar M, Haslam SM, North SJ, Panico M, Morris HR, Dell A, Wren BW, Aebi M, N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli, Science 298, 1790–3 (2002).Google Scholar
- 2.Young NM, Brisson JR, Kelly J, Watson DC, Tessier L, Lanthier PH, Jarrell HC, Cadotte N, St Michael F, Aberg E, Szymanski CM, Structure of the N-linked glycan present on multiple glycoproteins in the Gram-negative bacterium, Campylobacter jejuni, J Biol Chem 277, 42530–9 (2002).CrossRefPubMedGoogle Scholar
- 5.Stanley P, Narasimhan S, Siminovitch L, Schachter H, Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-glycoprotein N-acetylglucosaminyltransferase activity, Proc Natl Acad Sci USA 72, 3323–7 (1975).PubMedGoogle Scholar
- 9.Hase S, Ikenaka T, Matsushima Y. A highly sensitive method for analyses of oligosaccharides by tagging of the reducing end sugars with a fluorescent compound, J Biochem 9, 407–14 (1981).Google Scholar
- 11.Streisinger G, The Zebrafish Book, edited by Westerfield M (University of Oregon Press, Eugene, OR, 1995).Google Scholar