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Genetic analysis of oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) populations based on mitochondrial cox1 and nad1 gene sequences from India and other Asian countries

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Abstract

The study examined the genetic diversity and demographic history of Bactrocera dorsalis, a destructive and polyphagous insect pest of fruit crops in diverse geographic regions of India. 19 widely dispersed populations of the fly from India and other Asian countries were analysed using partial sequences of mitochondrial cytochrome oxidase I (cox1) and NADH dehydrogenase 1 (nad1) genes to investigate genetic diversity, genetic structure, and demographic history in the region. Genetic diversity indices [number of haplotypes (H), haloptype diversity (Hd), nucleotide diversity (π) and average number of nucleotide difference (k)] of populations revealed that B. dorsalis maintains fairly high level of genetic diversity without isolation by distance among the geographic regions. Demographic analysis showed significant (negative) Tajimas’ D and Fu’s F S with non significant sum of squared deviations (SSD) values, which indicate the possibility of recent sudden expansion of species and is further supported through distinctively star-like distribution structure of haplotypes among populations. Thus, the results indicate that both ongoing and historical factors have played important role in determining the genetic structure and diversity of the species in India. Consequently, sterile insect technique (SIT) could be a possible management strategy of species in the regions.

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Acknowledgments

We thank Dr. Mukesh Kumar (Gujarat), Sh. Shravan Naga (Rajasthan), Dr. S. K. Naik (Bihar) and Sh. Pramod Kumar (Odisha & Uttar Pradesh) for assistance in collection of samples from respective sites spanning over India. We also thank to Dr. Pankaj Sood, Department of Entomology, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh, India, Dr. Santosh S. Mali, ICAR-RCER, Research Centre, Plandu, Ranchi, Jharkhand, India and anonymous reviewers for their constructive comments and editing of final manuscript. We are grateful to Dr. B.P. Bhatt (Director of institute), Dr. A.K. Singh (Head of centre) and Dr. Bikash Das (Project leader) for giving valuable suggestions and providing laboratory facilities. This research was funded by Ministry of Agriculture, Government of India through National Initiative on Climate Resilient Agriculture (NICRA) project under Indian Council of Agricultural Research (ICAR) (ICAR-RCER/RC R/E.F./2011/29). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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  1. ICAR Research Complex for Eastern Region, Research Centre, Plandu, Ranchi, Jharkhand, 834010, India

    Jaipal S. Choudhary, Naiyar Naaz, Chandra S. Prabhakar & Moanaro Lemtur

  2. Department of Entomology, Veer Kunwar Singh College of Agriculture, Bihar Agricultural University, Sabour, Dumraon, Buxar, Bihar, 802136, India

    Chandra S. Prabhakar

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  1. Jaipal S. Choudhary
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Correspondence to Jaipal S. Choudhary.

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Choudhary, J.S., Naaz, N., Prabhakar, C.S. et al. Genetic analysis of oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) populations based on mitochondrial cox1 and nad1 gene sequences from India and other Asian countries. Genetica 144, 611–623 (2016). https://doi.org/10.1007/s10709-016-9929-7

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