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Genetica

, Volume 144, Issue 4, pp 469–476 | Cite as

Genetic structuring in a relictual population of screaming hairy armadillo (Chaetophractus vellerosus) in Argentina revealed by a set of novel microsatellite loci

  • Maximiliano Nardelli
  • Ezequiel Alejandro Ibáñez
  • Dara Dobler
  • Fabienne Justy
  • Frédéric Delsuc
  • Agustín Manuel Abba
  • Marcelo Hernán Cassini
  • Juan Ignacio Túnez
Article

Abstract

The screaming hairy armadillo (Chaetophractus vellerosus) is a mammal species containing disjunct and isolated populations. In order to assess the effect of habitat fragmentation and geographic isolation, we developed seven new microsatellite loci isolated from low-coverage genome shotgun sequencing data for this species. Among these loci, six microsatellites were found to be polymorphic with 8–26 alleles per locus detected across 69 samples analyzed from a relictual population of the species located in the northeast of the Buenos Aires Province (Argentina). Mean allelic richness and polymorphic information content were 15 and 0.75, with observed and expected heterozygosities ranging from 0.40 to 0.67 and 0.58 to 0.90, respectively. All loci showed departures from Hardy–Weinberg equilibrium. The analysis of population structure in this relictual population revealed three groups of individuals that are genetically differentiated. These newly developed microsatellites will constitute a very useful tool for the estimation of genetic diversity and structure, population dynamics, social structure, parentage and mating system in this little-studied armadillo species. Such genetic data will be particularly helpful for the development of conservation strategies for this isolated population and also for the endangered Bolivian populations previously recognized as a distinct species (Chaetophractus nationi).

Keywords

Molecular markers Armadillos Habitat fragmentation Molecular ecology 

Notes

Acknowledgments

We thank L.G. Pagano and M.C. Ezquiaga for their invaluable assistance during fieldwork. Our thanks also extend to the farm owners (Landa Family) and workers that allowed access to their property. Stéphane Garnier and two anonymous reviewers provided comments that helped improved a previous version of the manuscript.

Funding

This work has been financially supported by the Centre National de la Recherche Scientifique, Consejo Nacional de Investigaciones Científicas y Técnicas of Argentina (PIP No. 11420100100189), Agencia Nacional de Promoción Científica y Tecnológica of Argentina (PICT-2010-1978), Universidad Nacional de Luján, Argentina (Fondos Finalidad 3.5), and Universidad Nacional de La Plata. This study has benefited from an “Investissements d’Avenir” grant managed by Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01). This is contribution ISEM 2016-124-S of the Institut des Sciences de l’Evolution de Montpellier.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Maximiliano Nardelli
    • 1
  • Ezequiel Alejandro Ibáñez
    • 1
  • Dara Dobler
    • 1
  • Fabienne Justy
    • 2
  • Frédéric Delsuc
    • 2
  • Agustín Manuel Abba
    • 3
  • Marcelo Hernán Cassini
    • 1
    • 4
  • Juan Ignacio Túnez
    • 1
  1. 1.Departamento de Ciencias BásicasUniversidad Nacional de LujánLujánArgentina
  2. 2.Institut des Sciences de l’Evolution, UMR 5554, CNRS, IRD, EPHEUniversité de MontpellierMontpellierFrance
  3. 3.Centro de Estudios Parasitológicos y de Vectores (CEPAVE), CCT-CONICETUniversidad Nacional de La PlataLa PlataArgentina
  4. 4.Laboratorio de Biología del ComportamientoIBYME-CONICETBuenos AiresArgentina

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