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Mapping of yellow mosaic virus (YMV) resistance in soybean (Glycine max L. Merr.) through association mapping approach

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Abstract

Yellow Mosaic Virus (YMV) is a serious disease of soybean. Resistance to YMV was mapped in 180 soybean genotypes through association mapping approach using 121 simple sequence repeats (SSR) and four resistance gene analogue (RGA)-based markers. The association mapping population (AMP) (96 genotypes) and confirmation population (CP) (84 genotypes) was tested for resistance to YMV at hot-spot consecutively for 3 years (2007–2009). The genotypes exhibited significant variability for YMV resistance (P < 0.01). Molecular genotyping and population structure analysis with ‘admixture’ co-ancestry model detected seven optimal sub-populations in the AMP. Linkage disequilibrium (LD) between the markers extended up to 35 and 10 cM with r2 > 0.15, and >0.25, respectively. The 4 RGA-based markers showed no association with YMV resistance. Two SSR markers, Satt301 and GMHSP179 on chromosome 17 were found to be in significant LD with YMV resistance. Contingency Chi-square test confirmed the association (P < 0.01) and the utility of the markers was validated in the CP. It would pave the way for marker assisted selection for YMV resistance in soybean. This is the first report of its kind in soybean.

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Acknowledgments

First author sincerely acknowledges Post Graduate School, Indian Agricultural Research Institute, for providing the fellowship during the Post-Graduate study.

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Authors and Affiliations

  1. Cummings’s Lab., Directorate of Maize Research, Pusa Campus, New Delhi, 12, India

    Bhupender Kumar

  2. Division of Genetics, Indian Agricultural Research Institute, New Delhi, 12, India

    Bhupender Kumar, Akshay Talukdar, Khushbu Verma, G. D. Harish, Sarmrat Gowda, S. K. Lal, R. L. Sapra & K. P. Singh

  3. Molecular Cytogenetics and Tissue Culture Lab., Department of Crop Improvement, CSK Himachal Pradesh Agricultural University, Palampur, 176 062, India

    Indu Bala

Authors
  1. Bhupender Kumar
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  2. Akshay Talukdar
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  3. Khushbu Verma
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  4. Indu Bala
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  5. G. D. Harish
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  6. Sarmrat Gowda
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  7. S. K. Lal
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  8. R. L. Sapra
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  9. K. P. Singh
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Corresponding author

Correspondence to Akshay Talukdar.

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Kumar, B., Talukdar, A., Verma, K. et al. Mapping of yellow mosaic virus (YMV) resistance in soybean (Glycine max L. Merr.) through association mapping approach. Genetica 143, 1–10 (2015). https://doi.org/10.1007/s10709-014-9801-6

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  • DOI: https://doi.org/10.1007/s10709-014-9801-6

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