Evolutionary dynamics of 5S rDNA location in acridid grasshoppers and its relationship with H3 histone gene and 45S rDNA location
We analyze the chromosomal location of 5S rDNA clusters in 29 species of grasshoppers belonging to the family Acrididae. There was extensive variation among species for the number and location of 5S rDNA sites. Out of 148 sites detected, 75% were proximally located, 21.6% were interstitial, and only 3.4% were distal. The number of 5S rDNA sites per species varied from a single chromosome pair (in six species) to all chromosome pairs (in five species), with a range of intermediate situations. Thirteen chromosomes from eight species carried two 5S rDNA clusters. At intraspecific level, differences among populations were detected in Eyprepocnemis plorans, and some heteromorphisms have also been observed in some species. Double FISH for 5S rDNA and H3 histone gene DNA, performed on 17 of these 29 species, revealed that both markers are sometimes placed in a same chromosome but at different location, whereas they appeared to co-localize in five species (Calliptamus barbarus, Heteracris adpersa, Aiolopus strepens, Oedipoda charpentieri and O. coerulescens). Double fiber-FISH in A. strepens and O. coerulescens showed that the two DNAs are closely interspersed with variable relative amounts of both classes of DNA. Finally, no correlation was observed between the number of 5S and 45S rDNA clusters in 23 species where this information was available. These results are discussed in the light of possible mechanisms of spread that led to the extensive variation in the number of clusters observed for both rDNA types in acridid grasshoppers.
KeywordsFluorescence in situ hybridization Grasshopper Histone genes Multigene families rDNA
This study was supported by grants from the Spanish Ministerio de Ciencia y Tecnología (CGL2009-11917) and Plan Andaluz de Investigación (CVI-6649), and was partially performed by FEDER funds. The scientific scholarship of Cabral-de-Mello DC was granted by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil).
- Cabral-de-Mello DC, Moura RC, Martins C (2010) Chromosomal mapping of repetitive DNAs in the beetle Dichotomius geminatus provides the first evidence for an association of 5S rRNA and histone H3 genes in insects, and repetitive DNA similarity between the B chromosome and A complement. Heredity 104:393–400PubMedCrossRefGoogle Scholar
- Cabral-de-Mello DC, Martins C, Souza MJ, Moura RC (2011a) Cytogenetic mapping of 5S and 18S rRNAs and H3 histone genes in four ancient Proscopiidae grasshopper species: contribution to understanding the evolutionary dynamics of multigene families. Cytogenet Genome Res 132:89–93PubMedCrossRefGoogle Scholar
- Descamps M (1973) Révision des Eumastacoidea aux échelons des familles et des sous-familles (genitalia, répartition, phylogénie). Acrida 2:161–298Google Scholar
- Eirín-López JM, Ruiz MF, González-Tizón AM, Martínez A, Sánchez L, Méndez J (2004) Molecular evolutionary characterization of the mussel Mytilus histone multigene family: first record of a tandemly repeated unit of five histone genes containing an H1 subtype with ‘‘orphon’’ features. J Mol Evol 58:131–144PubMedCrossRefGoogle Scholar
- Gillespie JJ, Johnston JS, Cannone JJ, Gutell RR (2006) Characteristics of the nuclear (18S, 5.8S, 28S and 5S) and mitochondrial (12S and 16S) rRNA genes of Apis mellifera (Insecta: Hymenoptera): structure, organization, and retrotransposable elements. Insect Mol Biol 15:657–686PubMedCrossRefGoogle Scholar
- Martíns C, Wasko AP (2004) Organization and evolution of 5S ribosomal DNA in the fish genome. In: Williams CR (ed) Focus on genome research. Nova Science Publishers, Hauppauge, pp 289–318Google Scholar
- Vitturi R, Colomba M, Mandrioli M, Pirrone AM (2002) rDNA (18S–28S and 5S) co-localization and linkage between ribosomal genes and (TTAGGG)n telomeric sequence in the earthworm Octodrilus complanatus (Annelida: Oligochaeta: Lumbricidae) revealed by single- and double-colour FISH. J Hered 93:279–282PubMedCrossRefGoogle Scholar