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Genetica

, Volume 138, Issue 7, pp 695–708 | Cite as

Mammalian carboxylesterase 3: comparative genomics and proteomics

  • Roger S. Holmes
  • Laura A. Cox
  • John L. VandeBerg
Article

Abstract

At least five families of mammalian carboxylesterases (CES) catalyse the hydrolysis or transesterification of a wide range of drugs and xenobiotics and may also participate in fatty acyl and cholesterol ester metabolism. In this study, in silico methods were used to predict the amino acid sequences, secondary and tertiary structures, and gene locations for CES3 genes and encoded proteins using data from several mammalian genome projects. Mammalian CES3 genes were located within a CES gene cluster with CES2 and CES6 genes, usually containing 13 exons transcribed on the positive DNA strand. Evidence is reported for duplicated CES3 genes for the chimp and mouse genomes. Mammalian CES3 protein subunits shared 58–97% sequence identity and exhibited sequence alignments and identities for key CES amino acid residues as well as extensive conservation of predicted secondary and tertiary structures with those previously reported for human CES1. The human genome project has previously reported CES3 mRNA isoform expression in several tissues, particularly in colon, trachea and in brain. Predicted human CES3 isoproteins were apparently derived from exon shuffling and are likely to be secreted extracellularly or retained within the cytoplasm. Mouse CES3-like transcripts were localized in specific regions of the mouse brain, including the cerebellum, and may play a role in the detoxification of drugs and xenobiotics in neural tissues and other tissues of the body. Phylogenetic analyses demonstrated the relationships and potential evolutionary origins of the mammalian CES3 family of genes which were related to but distinct from other mammalian CES gene families.

Keywords

Mammals Amino acid sequence Carboxylesterase Evolution Gene duplication 

Notes

Acknowledgments

This project was supported by NIH Grants P01 HL028972 and P51 RR013986. In addition, this investigation was conducted in facilities constructed with support from Research Facilities Improvement Program Grant Numbers 1 C06 RR13556, 1 C06 RR15456, 1 C06 RR017515. We gratefully acknowledge the assistance of Dr B. Patel in studying the phylogeny of CES3 and related CES gene families.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Roger S. Holmes
    • 1
    • 2
    • 3
  • Laura A. Cox
    • 1
    • 2
  • John L. VandeBerg
    • 1
    • 2
  1. 1.Department of GeneticsSouthwest Foundation for Biomedical ResearchSan AntonioUSA
  2. 2.Southwest National Primate Research CenterSouthwest Foundation for Biomedical ResearchSan AntonioUSA
  3. 3.School of Biomolecular and Physical SciencesGriffith UniversityNathanAustralia

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