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Understanding the genetic structure of Symplocos laurina Wall. Populations using nuclear gene markers

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Abstract

To characterize the genetic diversity of present populations of Symplocos laurina, which grow in the montane forests in India, we analyzed the DNA sequences of a nuclear gene. Using the 881 bp sequence of cytosolic Glyceraldehyde-3-phosphate dehydrogenase gene, we detected 24 haplotypes among 195 individuals sampled from 14 populations. Two dominant haplotypes were distributed over the entire range of this species in India and several private haplotypes were found. Low genetic diversity within population, high differentiation, number of population specific haplotypes and deviation from neutral evolution characterized the present populations of S. laurina. An analysis of molecular variance indicated the presence of geographic structure within the haplotype distribution. The occurrence of S. laurina preglaciation in India is the most parsimonious explanation for the current geographic structure observed. The populations are presumably ancient and might have spread across its extant distribution range in India through a recent range expansion event.

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Acknowledgments

The authors thank Ram Kulkarni from National Chemical Laboratory, Dr. B G Kulkarni and Dr. P S N Rao from Botanical Survey of India, Western Circle, Pune and H S Suresh (Center for Ecological Science, Indian Institute of Science, Bangalore) for the help rendered during sampling. Authors will like to thank Dr. Abhay Harsulkar and Rupali Khadke (Interactive Research School of Health Affairs, Pune) for their help in designing the experiments. We also thank the staff at various field stations and Forest Departments for their co-operation in sampling. We thank Dr. Rebecca Zwart from the National Chemical Laboratory, for her help in refining the manuscript. SB thanks the Council of Scientific and Industrial Research (CSIR), New Delhi for her research fellowship. This project was supported by a grant from the Department of Biotechnology, New Delhi to NCL and BSI.

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  1. Plant Molecular Biology Group, Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411008, India

    Sofia Banu, R. M. Bhagwat, N. Y. Kadoo, M. D. Lagu & V. S. Gupta

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  1. Sofia Banu
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  2. R. M. Bhagwat
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Correspondence to V. S. Gupta.

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Banu, S., Bhagwat, R.M., Kadoo, N.Y. et al. Understanding the genetic structure of Symplocos laurina Wall. Populations using nuclear gene markers. Genetica 138, 197–210 (2010). https://doi.org/10.1007/s10709-009-9410-y

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  • DOI: https://doi.org/10.1007/s10709-009-9410-y

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