, Volume 126, Issue 1–2, pp 101–110 | Cite as

Mobilization of a Hobo-related Sequence in the Genome of Drosophila simulans

  • F. P. Torres
  • L. F. M. Fonte
  • V. L. S. Valente
  • E. L. S. Loreto


The hobo transposable element can occur under three forms in the Drosophila genome: as a complete element (also called canonical), as internally deleted copies, or as hobo-related sequences (relics). Some evidence indicated that canonical elements and internally deleted copies are recent acquisitions of Drosophila genomes, while the “relics” are old components, normally degenerated and immobile. Here we present the characterization of a hobo-related sequence, found in the genome of a hypermutable strain of D. simulans, which insertion into the white locus raised a de novo white mutation. It is a shorter hobo related element presenting, overall, roughly 18% of divergence at the DNA level from the canonical hobo, with many indels that make clear this element is defective. However, its ITRs and flanking regions are extremely conserved. This is the first hobo “relic” showed to be mobilizable. We suggest, and point up some evidences, toward the idea that this sequence could have been mobilized by the canonical element. The presence of a similar “relic” element in D. sechellia allows us to suggest that these elements have been maintained mobilizable since the time of divergence between these species.


canonical hobo cross mobilization hobo relic hypermutable strain transposable elements 


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  1. Atkinson, P.W., Warren, W.D., O’Brochta, D.A. 1993The hobo transposable element of Drosophila can be cross-mobilized in houseflies and excises the Ac element of maizeProc. Natl. Acad. Sci. U.S.A.9096939697PubMedGoogle Scholar
  2. Blackman, R.K., Kochlerm, M.M., Grimaila, R., Gelbart, W.M. 1989Identification of a fully functional hobo transposable element and its use for germ-line transformation of DrosophilaEMBO J.8211217PubMedGoogle Scholar
  3. Borsatti, F., Azzoni, P., Mandrioli, M. 2003Identification of a new hobo element in the cabbage moth, Mamestra  brassicae (Lepidoptera)Hereditas139151155CrossRefPubMedGoogle Scholar
  4. Boussy, I.A., Itoh, M. 2004Wanderings of hobo: a transposon in Drosophila melanogaster and its close relativesGenetica120125136CrossRefPubMedGoogle Scholar
  5. Boussy, I.A., Daniels, S.B. 1991hobo transposable elements in Drosophila melanogaster and D. simulansGenet. Res.582734PubMedGoogle Scholar
  6. Calvi, B.R., Hong, T.J., Findley, S.D., Gelbart, W.M. 1991Evidence for a common evolutionary origin of inverted repeat transposons in Drosophila and plants: hobo, Activator, and Tam3Cell66465471CrossRefPubMedGoogle Scholar
  7. Daniels, S.B., Chovnick, A., Boussy, I.A. 1990Distribution of hobo transposable elements in the genus DrosophilaMol. Biol. Evol.7589606PubMedGoogle Scholar
  8. DeVault, J.D., Narang, S.K. 1994Transposable elements in Lepidoptera: hobo-like transposons in Heliothis  virescens and Helicoverpa  zeaBiochem. Biophys. Res.203169175CrossRefGoogle Scholar
  9. Engels, W.R. 1989P element in Drosophila melanogasterBerg, D.E.Howe,  M.M. eds. Mobile DNAAm. Soc. MicrobiolWashington DC437484Google Scholar
  10. Finnegan, D.J. 1989Eukaryotic transposable elements and genome evolutionTIG5103107PubMedGoogle Scholar
  11. Galindo, M.I., Bigot, Y., Sánchez, M.D., Periquet, G., Pascual, L. 2001Sequences homologous to the hobo transposable element in E strains of Drosophila melanogasterMol. Biol. Evol.1815321539PubMedGoogle Scholar
  12. Handler, A.M., Gomez, S.P. 1996The hobo transposable element excises and has related elements in tephritid speciesGenetics14313391347PubMedGoogle Scholar
  13. Inoue, Y.H., Yamamoto, M. 1987Insertional DNA and spontaneous mutation at white locus in Drosophila simulansMol. Gen. Genet.20994100CrossRefGoogle Scholar
  14. Inoue, Y.H., Taira, T., Yamamoto, M. 1988Genetics of an unstable white mutant in Drosophila simulans: reversion, suppression and somatic instabilityGenetics119903912PubMedGoogle Scholar
  15. Jowett, T. 1986Preparation of nucleic acidsRoberts, D.B. eds. Drosophila: A Practical ApproachIRL PressOxford275278Google Scholar
  16. Lachaise, D., Silvain, J.-F. 2004How two Afrotropical endemics made two cosmopolitan human commensals: the Drosophila melanogasterD. simulans palaeogeographic riddleGenetica1201739CrossRefPubMedGoogle Scholar
  17. Lerat, E., Rizzon, C., Biémont, C. 2003Sequence divergence within transposable element families in the Drosophila melanogaster genomeGenome Research1318891896PubMedGoogle Scholar
  18. Loreto, E.L.S., Basso da Silva, L., Zaha, A., Valente, V.L.S. 1998aDistribution of transposable elements in neotropical species of DrosophilaGenetica.101153165Google Scholar
  19. Loreto, E.L.S., Zaha, A., Nichols, C., Pollock, J.A., Valente, V.L.S. 1998bCharacterization of a hypermutable strain of Drosophila simulansCell. Mol. Life Sci.5412831290Google Scholar
  20. Loreto, E.L.S., Zaha, A., Valente, V.L.S. 1998Transposable elements in South American populations of Drosophila simulansGenet. Select. Evol.30171180Google Scholar
  21. McGinnis, W., Shermoen, A.W., Beckendorf, S.K. 1983A transposable element inserted just 5′ to a Drosophila glue protein gene alters gene expression and chromatin structureCell347584CrossRefPubMedGoogle Scholar
  22. Mullins, M.C., Rio, D.C., Rubin, G.M. 1989Cis-acting DNA sequences requirement of Drosophila P element transposase in vivoGenes. Dev.3729738PubMedGoogle Scholar
  23. Pascual, L., Periquet, G. 1991Distribution of hobo transposable elements in natural populations of Drosophila melanogasterMol. Biol. Evol.8282296PubMedGoogle Scholar
  24. Periquet, G., Lemeunier, F., Bigot, Y., Hamelin, M.H., Bazin, C., Ladevèze, V., Eeken, J., Galindo, M.I., Pascual, L., Boussy, I. 1994The evolutionary genetics of the hobo transposable element in the Drosophila melanogaster complexGenetica937990CrossRefPubMedGoogle Scholar
  25. Periquet, G., Hamelin, M.H., Kalmes, R., Eeken, J. 1990hobo elements and their deletion-derivative sequences in Drosophila melanogaster and its sibling species D. simulans, D. mauritiana and D. sechelliaGenet. Select. Evol.22393402Google Scholar
  26. Periquet, G., Hamelin, M.H., Bigot, Y., Hu, K. 1989aPresence of the deleted hobo element Th in Eurasian population of Drosophila melanogasterGenet. Select. Evol.21107111Google Scholar
  27. Periquet, G., Hamelin, M.H., Bigot, Y., Lepissier, A. 1989bGeographical and historical patterns of distribution of hobo elements in Drosophila melanogaster populationsJ. Evol. Biol.2223229CrossRefGoogle Scholar
  28. Rubin, G.M., Spradling, A.C. 1982Genetic transformation of Drosophila with transposable element vectorScience218348353PubMedGoogle Scholar
  29. Simmons, G.M. 1992Horizontal transfer of hobo transposable elements within the Drosophila melanogaster species complex: evidence from DNA sequencingMol. Biol. Evol.910501060PubMedGoogle Scholar
  30. Simmons, G.M., Plummer, D., Simon, A., Boussy, I.A., Frantsve,  J., Itoh, M. 1998Horizontal and vertical transmission of hobo-related sequences between Drosophila melanogaster and Drosophila simulansSyvanen, M.Kado,  C.I. eds. Horizontal Gene TransferChapman & HallNew York285294Google Scholar
  31. Staden, R. 1996The Staden sequence analysis packageMol. Biotechnol.5233241PubMedGoogle Scholar
  32. Streck, R.D., MacGaffey, J.E., Beckendorf, S.K. 1986The structure of hobo transposable elements and their insertion sitesEMBO J.536153623PubMedGoogle Scholar
  33. Sundararajan, P., Atkinson, P.W., O’Brochta, D.A. 1999Transposable element interactions in insects: cross mobilization of hobo and HermesInsect Mol. Biol.8359368CrossRefPubMedGoogle Scholar
  34. Thummel, C.S., Pirrota, V. 1992Technical notes: new pCaSpeR P-element vectorsDros. Inf. Serv.71150Google Scholar
  35. Torti, C., Gomulski, L.M., Bonizzoni, M., Murelli, V., Moralli, D., Guglielmino, C.R., Raimondi, E., Crisafulli, D., Capy, P., Gasperi, G., Malacrida, R. 2005Cchobo, a hobo-related sequence in Ceratitis  capitataGenetica123313325CrossRefPubMedGoogle Scholar
  36. Wang, W., Yu, H., Long, M. 2004Duplication-degeneration as a mechanism of gene fission and the origin of new genes in Drosophilia speciesNature Genetics3523527Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • F. P. Torres
    • 1
  • L. F. M. Fonte
    • 2
  • V. L. S. Valente
    • 1
  • E. L. S. Loreto
    • 1
    • 3
  1. 1.Programa de Pós-Graduação em Genética e Biologia Molecular, Departamento de GenéticaUniversidade Federal do Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  2. 2.Curso de Ciências BiológicasUniversidade Federal do Rio Grande do Sul (UFRGS)Porto AlegreBrazil
  3. 3.Departamento de BiologiaUniversidade Federal de Santa Maria (UFSM)Santa MariaBrazil

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