Nutrient Cycling in Agroecosystems

, Volume 101, Issue 3, pp 391–400 | Cite as

Soil enzyme activities with biomass ashes and phosphorus fertilization to rice–wheat cropping system in the Indo-Gangetic plains of India

  • Sandeep Sharma
  • H. S. Thind
  • Yadvinder Singh
  • Varinderpal Singh
  • Bijay Singh
Original Article


Bagasse ash (BA) and rice husk ash (RHA) contain significant quantities of P and many other nutrients. Application of biomass ashes has been shown to improve crop yields and soil properties. The present experiment was conducted to determine the effect of BA and RHA application in combination with different doses of P fertilizer on soil enzyme activities in rice–wheat rotation in the Indo-Gangetic plains of India. Both BA and RHA significantly increased the dehydrogenase, alkaline phosphatase and phytase activities as compared to no ash application in the 0–0.15 m soil layer. Activities of these enzymes also increased with P fertilization. Biomass ashes and P fertilizers showed little effect on enzymatic activities in the 0.15–0.30 m soil layer. Application of biomass ashes and P fertilization significantly increased Olsen-P and organic carbon content in soil over control. Enzyme activities were significantly and positively correlated with Olsen-P, organic carbon content and wheat grain yield. This study suggests that soil quality can be improved by applying biomass ashes and P fertilization on a loamy sand soil under rice–wheat system.


Biomass ashes Dehydrogenase Alkaline phosphatase Phytase Rice–wheat 


  1. Ames BN (1966) Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol 8:115–118CrossRefGoogle Scholar
  2. Anonymous (2012) Package of practices for crops of Punjab. Punjab Agricultural University, LudhianaGoogle Scholar
  3. Barros N, Salgado J, Feijoo S (2007) Calorimetry and soil. Thermochim Acta 458:11–17CrossRefGoogle Scholar
  4. Bastida F, Zsolnay A, Hernandez T, Garcia C (2008) Past, present and future of soil quality indices: a biological perspective. Geoderma 147:159–171CrossRefGoogle Scholar
  5. Benbi DK, Brar JS (2009) A 25-year record of carbon sequestration and soil properties in intensive agriculture. Agron Sustain Dev 29:257–265CrossRefGoogle Scholar
  6. Beri V, Gupta AP (2003) Recycling of rural and urban organic wastes: a review. Department of Soil Science, Punjab Agricultural University, LudhianaGoogle Scholar
  7. Bhattacharya SS, Chattopadhyay GN (2002) Increasing bioavailability of phosphorus from fly ash through vermicomposting. J Environ Qual 31:2116–2119CrossRefPubMedGoogle Scholar
  8. Bonmati M, Ceccanti B, Nannipieri P (1991) Spatial variability of phosphatase, urease, protease, organic carbon and total nitrogen in soil. Soil Biol Biochem 23:391–396CrossRefGoogle Scholar
  9. Bougnom BP, Insam H (2009) Ash additives to compost affect soil microbial communities and apple seedling growth. Die Bodenkultur 60:5–15Google Scholar
  10. Buyanovsky GA, Wagner GH (1998) Carbon cycling in cultivated land and its global significance. Glob Change Biol 4:131–141CrossRefGoogle Scholar
  11. Casida LE, Kklein DA, Santoro T (1964) Soil dehydrogenase activity. Soil Sci 98:371–376CrossRefGoogle Scholar
  12. Cavazzna J, Volk T (1996) Assessing long-term impacts of increasing crop productivity on atmospheric CO2. Energy Policy 24:403–411CrossRefGoogle Scholar
  13. Demeyer A, Nkana JCV, Verloo MG (2001) Characteristics of wood ash and influence on soil properties and nutrient uptake: an overview. Bioresour Technol 77:287–295CrossRefPubMedGoogle Scholar
  14. Demoling LA, Baath E, Greve G, Wouterse M, Schmitt H (2009) Effects of sulfamethoxazole on soil microbial communities after adding substrate. Soil Biol Biochem 41:840–848CrossRefGoogle Scholar
  15. Dick RP, Tabatabai MA (1986) Hydrolysis of polyphosphates by corn roots. Plant Soil 94:247–256CrossRefGoogle Scholar
  16. Dick RP, Breakwell DP, Turco RF (1996) Soil enzyme activities and biodiversity measurements as integrative microbiological indicators. In: Doran JW, Jones AJ (eds) Methods for assessing soil quality. Soil Science Society of America, Madison, pp 247–271Google Scholar
  17. Ding XL, Zhang B, Zhang XD, Yang XM, Zhang XP (2011) Effects of tillage and crop rotation on soil microbial residues in a rainfed agroecosystem of northeast China. Soil Till Res 11:43–49CrossRefGoogle Scholar
  18. Duff SMG, Sarath G, Plaxto WC (1994) The role of acid phosphatases in plant phosphorus metabolism. Physiol Plant 90:791–800CrossRefGoogle Scholar
  19. Fassel VA, Kinseley RN (1974) Inductively coupled plasma. Anal Chem 46:1155–1164CrossRefGoogle Scholar
  20. Garcia C, Hernandez T, Costa F (1994) Microbial activity in soils under Mediterranean environmental conditions. Soil Biol Biochem 26:1185–1191CrossRefGoogle Scholar
  21. Garcia C, Hernandez MT, Costa F (1997) Potential use of dehydrogenase activity as an index of microbial activity in degraded soils. Commun Soil Sci Plant Anal 28:123–134CrossRefGoogle Scholar
  22. Greaves MP, Webley DM (1965) A study of the breakdown of organic phosphates by micro-organisms from the root region of certain pasture grasses. J Appl Bacteriol 28:454–465CrossRefPubMedGoogle Scholar
  23. Gregorich EG, Carte RM, Doran JW, Pankhurs CE, Dwyer LM (1997) Biological attributes of soil quality. In: Gregorich E, Carter MR (eds) Soil quality for crop production and ecosystem health. Elsevier, Amsterdam, pp 81–113CrossRefGoogle Scholar
  24. International Rice Research Institute (IRRI) (2000) IRRISTAT for window (CD-ROM) version 4.02b. Los Baños, Philippines, IRRIGoogle Scholar
  25. Kapralek F (1986) Fyziologie bakterii (Physiology of bacteria). SPN Praha, 603 pp (in Czech)Google Scholar
  26. Kuligowski K, Poulsen TG, Rubaek GH, Sorensen P (2010) Plant-availability to barley of phosphorus in ash from thermally treated animal manure in comparison to other manure based materials and commercial fertilizer. Eur J Agron 33:293–303CrossRefGoogle Scholar
  27. Ladha JK, Dawe D, Pathak H, Padre AT, Yadav RL, Singh Y, Singh B, Singh P, Kundu AL, Sakal R, Ram N, Regmi AP, Gami SK, Bhandari AL, Amin K, Yadav CR, Bhattarai EM, Gupta RK, Hobbs PR (2003) How extensive are yield declines in long-term rice–wheat experiments in Asia? Field Crop Res 81:159–180CrossRefGoogle Scholar
  28. Lopez R, Padilla E, Bachmann S, Eichler-Loebermann B (2009) Effects of biomass ashes on plant nutrition in tropical and temperate regions. J Agri Rur Deve Trop Subtrop 110:51–60Google Scholar
  29. Melero S, Madejon E, Ruiz JC, Herencia JF (2007) Chemical and biochemical properties of a clay soil under dryland agriculture system as affected by organic fertilization. Eur J Agron 26:327–334CrossRefGoogle Scholar
  30. Mozaffari M, Russelle MP, Rose C et al (2002) Nutrient supply and neutralizing value of alfalfa stem gasification ash. Soil Sci Soc Am J 66:171–178CrossRefGoogle Scholar
  31. Nahas E, Centurion JF, Assis C (1994) Efeito das caracteristicas quimicas dos solos sobre os microorganismos solubilizatores de fosfato e produtores de fosfatases (Effect of chemical properties of soil on phosphate solubilizing and phosphatase-producting microorganisms). Revista Brasileira de Ciencia do Solo. 18:49–54Google Scholar
  32. Nannipieri P, Cervelli S, Perna A (1973) Enzyme activities in some Italian soils. Agric Ital Pisa 73:367–376Google Scholar
  33. Nybroe O, Jorgensen PE, Henze M (1992) Enzyme activities in waste water and activated sludge. Water Res 26:579–584CrossRefGoogle Scholar
  34. Olsen SR, Cole CV, Watanabe FS, Dean IA (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. U.S. Department of Agriculture Circle 939:1–19Google Scholar
  35. Pagliari P, Rosena C, Strocka J, Russellea M (2010) Phosphorus availability and early corn growth response in soil amended with Turkey manure ash. Comm Soil Sci Plant Anal 41:1369–1382CrossRefGoogle Scholar
  36. Patterson SJ, Acharya SN, Thomas JE et al (2004) Integrated soil and crop management: Barley biomass and grain yield and canola seed yield response to land application of wood ash. Agron J 96:971–977CrossRefGoogle Scholar
  37. Perucci P, Monac E, Casucci C et al (2006) Effect of recycling wood ash on microbiological and biochemical properties of soil. Agron Sustain Dev 26:157–165CrossRefGoogle Scholar
  38. Quilchano C, Maranon T (2002) Dehydrogenase activity in Mediterranean forest soils. Biol Fertil Soils 35:102–107CrossRefGoogle Scholar
  39. Richards LA (1954) Diagnosis and improvement of saline and alkali soils. USDA agriculture handbook no. 60, 83. USDA, Washington, pp 49–50Google Scholar
  40. Saarsalmi A, Malkonen E, Piirainen S (2001) Effects of wood ash fertilization on forest soil chemical properties. Silva Fennica 35:355–368CrossRefGoogle Scholar
  41. Sander ML, Andren O (1997) Ash from cereal and rape straw used for heat production: liming effect and contents of plant nutrients and heavy metals. Water Air Soil Pollut 93:93–108Google Scholar
  42. Schiemenz K, Eichle-Loebermann B (2010) Biomass ashes and their phosphorus fertilizing effect on different crops. Nutr Cycl Agroecosyst 87:471–482CrossRefGoogle Scholar
  43. Solaimalai A, Baska RM, Ramesh PT, Ravisanka N (2001) Utilization of press mud as soil amendment and organic manure—a review. Agric Rev 22:25–32Google Scholar
  44. Speir TW, Cowling JC (1991) Phosphatase activities of pasture plants and soils: relationship with plant productivity and soil P fertility indices. Biol Fertil Soils 12:189–194CrossRefGoogle Scholar
  45. Sreeramulu G, Srinivasa DS, Joseph R (1996) Lactobacillus amylovorus as a phytase producer in submerged culture. App Microbio 23:385–388CrossRefGoogle Scholar
  46. Tabatabai MA, Bremner JM (1969) Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307CrossRefGoogle Scholar
  47. Tadano T, Ozawa K, Sakai H, Osaki M, Matsui H (1993) Secretion of acid phosphatase by the roots of crop plants under phosphorus—deficient conditions and some properties of the enzyme secreted by lupin roots. Plant Soil 155(156):95–98CrossRefGoogle Scholar
  48. Tarafdar JC, Marschner H (1994) Phosphatase activity in the rhizosphere and hyphosphere of VA mycorrhizal wheat supplied with inorganic and organic phosphorous. Soil Biol Biochem 26:387–395CrossRefGoogle Scholar
  49. Tarafdar JC, Marschner H (1995) Dual inoculation with Aspergillus fumigatus and Glomus mosseae enhances biomass production and nutrient uptake in wheat (Triticum aestivum L.) supplied with organic phosphorus as Na-phytate. Plant Soil 173:97–102CrossRefGoogle Scholar
  50. Thind HS, Singh Y, Singh B, Singh Varinderpal, Sharma S, Monika Vashistha, Gobinder Singh (2012) Land application of rice husk ash, bagasse ash and coal fly ash: effects on crop productivity and nutrient uptake in rice–wheat system on an alkaline loamy sand. Field Crops Res 135:137–144CrossRefGoogle Scholar
  51. Truu M, Truu J, Ivask M (2008) Soil microbiological and biochemical properties for assessing the effect of agricultural management practices in Estonian cultivated soils. Eur J Soil Biol 44:231–237Google Scholar
  52. Vance ED, Mitchell CC (2000) Beneficial use of wood ash as an agricultural soil amendment: case studies from the United States forest products industry. In: Power JF, Dick WA (eds) Land application of agricultural, industrial and municipal by-products. SSSA, Madison, pp 567–582Google Scholar
  53. Verma G, Mathur AK (2009) Effect of integrated nutrient management on active pools of soil organic matter under maize-wheat system of a Typic Haplustept. J Indian Soc Soil Sci 57:317–322Google Scholar
  54. Walkley A, Black JA (1934) An examination of the Degtjareff method for determining soil organic matter and a proposed modification of the chromic acid titration method. Soil Sci 37:29–38CrossRefGoogle Scholar
  55. Yadav BK, Tarafdar JC (2004) Phytase activity in the rhizosphere of crops, trees and grasses under arid environment. J Arid Environ 58:285–293CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Sandeep Sharma
    • 1
  • H. S. Thind
    • 1
  • Yadvinder Singh
    • 1
  • Varinderpal Singh
    • 1
  • Bijay Singh
    • 1
  1. 1.Department of Soil SciencePunjab Agricultural UniversityLudhianaIndia

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