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Evaluation of seminal plasma composition and spermatozoa quality parameters of silver barb, Barbonymus gonionotus Bleeker, 1850

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Abstract

Seminal composition and semen quality are the important determinants in assessing the reproductive performance of different fishes. This study was carried out to evaluate the seminal composition and sperm quality of Barbonymus gonionotus. The seminal plasma contained 17.2 ± 0.34 mmol/l, 20.9 ± 0.48 mmol/l, 0.72 ± 0.04 mmol/l, 3.8 ± 0.2 mmol/l, and 1.49 ± 0.02 g/dl of Na+, K+, Ca++, Mg++, and total protein, respectively. The physical spermatological parameters, such as sperm volume, sperm motility, motility duration, sperm density, osmolality, and pH values were 1.55 ± 0.15 ml, 89 ± 2%, 391.9 ± 8.5 s, 2.8 ± 0.2 × 1010 /ml, 400.6 ± 5.1 mmol/kg, and 8.75 ± 0.10, respectively. In correlation matrix, the K+ (R2 = 0.39, P < 0.01) and Ca++ (R2 = 0.27, P < 0.05) ions and osmolality (R2 = 0.29, P < 0.05) showed significant positive correlations with sperm motility. Similarly, fertilization rate significantly influenced by sperm motility (R2 = 0.26, P < 0.05) and K+ (R2 = 0.30, P < 0.05) and Ca++ (R2 = 0.26, P < 0.05) ions. Also, osmolality significantly and negatively correlated with Mg++ (R2 = 0.33, P < 0.05) and sperm motility duration (R2 = 0.28, P < 0.05). Therefore, based on this results, it can be concluded that seminal plasma ions, K+ and Ca++ and osmolality are the key factors for the determination of sperm quality of silver barb, and these parameters could be considered during standardization of artificial fertilization or cryopreservation technique of silver barb spermatozoa.

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Acknowledgements

Authors would like to thank to Prof. Dr. GKM Mustafizur Rahman for his kind help during sample analysis and to Dr. Hamid Niksirat for his valuable guidelines during manuscript preparation.

Funding

This study is supported by the Research Management Committee (RMC) of Bangabandhu Sheikh Mujibur Rahaman Agricultural University (Grant No. UGC/RMC-55, Section-8) and the Ministry of Education, Youth and Sports of the Czech Republic—projects “CENAKVA” (No. CZ.1.05/2.1.00/01.0024), “CENAKVA II” (No. LO1205 under the NPU I program), and by the Grant Agency of University of South Bohemia (No. 017/2016/Z).

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  1. Ibrahim Rashid and Md Shakhawate Hossain contributed equally to this work.

Authors and Affiliations

  1. Department of Fisheries Biology and Aquatic Environment, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh

    Ibrahim Rashid, Md Shakhawate Hossain & S. M. Rafiquzzaman

  2. Faculty of Fisheries and Protection of Waters, South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic

    Md Shakhawate Hossain

  3. Department of Genetics and Fish Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh

    Mohammad Abdus Salam

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Rashid, I., Hossain, M.S., Salam, M.A. et al. Evaluation of seminal plasma composition and spermatozoa quality parameters of silver barb, Barbonymus gonionotus Bleeker, 1850. Fish Physiol Biochem 45, 105–114 (2019). https://doi.org/10.1007/s10695-018-0539-4

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