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Fish Physiology and Biochemistry

, Volume 45, Issue 1, pp 63–70 | Cite as

Oxidative stress mediated the inhibition of cerebral creatine kinase activity in silver catfish fed with aflatoxin B1-contaminated diet

  • Carine F. Souza
  • Matheus D. BaldisseraEmail author
  • Carla C. Zeppenfeld
  • Sharine Descovi
  • Lenita M. Stefani
  • Bernardo Baldisserotto
  • Aleksandro S. da Silva
Article
  • 117 Downloads

Abstract

Aflatoxin B1 (AFB1) is an environmental toxicant and neurotoxic compound that induces the production of free radicals, causing oxidative stress. Creatine kinase (CK) is a central controller of energy metabolism in tissues with a large and fluctuating energy demand, and it is highly susceptible to inactivation by free radicals and oxidative damage. Thus, the aim of this study was to evaluate whether a diet for freshwater silver catfish (Rhamdia quelen) containing AFB1 inhibits cerebral CK activity, as well as the involvement of the oxidative stress on this inhibition. Brain CK activity was lower on days 14 and 21 post-feeding in animals that received AFB1-contaminated diet compared to the control group (basal diet), similarly to the brain sodium-potassium pump (Na+, K+-ATPase) activity. On the other hand, lipid peroxidation and protein carbonylation levels were higher on days 14 and 21 post-feeding in animals fed with AFB1-contaminated feed compared to the control group, while the antioxidant capacity against peroxyl radicals and thiol content was lower. Based on these evidences, the data demonstrated that diet containing AFB1 severely affects CK activity, an essential enzyme that plays an important role in brain energy homeostasis. Also, the impairment of energetic homeostasis linked with the use and generation of ATP via inhibition of CK activity elicited an inhibition of enzymes ATP-dependent, such as Na+, K+-ATPase. Moreover, the inhibition of brain CK activity appears to be mediated by the oxidation of lipids, proteins, and thiol group, as well as by a reduction in the antioxidant capacity.

Keywords

AFB1 Adenosine triphosphate Mycotoxins Phosphotransfer network Rhamdia quelen 

Notes

Funding information

This study received financial support from the National Council for Scientific and Technological Development (CNPq) (grant number 301156/2012-3) and received research fellowship for B. Baldisserotto. The Coordination for the Improvement of Higher Education Personnel (CAPES) provided two PhD fellowships for M.D. Baldissera and C.F. Souza.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical note

The methodology used in the experiment was approved by the Ethical and Animal Welfare Committee of the Universidade do Estado de Santa Catarina under protocol number 9912090817. All applicable national guidelines for the care and use of animals were followed.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Physiology and PharmacologyUniversidade Federal de Santa MariaSanta MariaBrazil
  2. 2.Graduate Program in Toxicological Biochemistry and Molecular BiologyUniversidade Federal de Santa MariaSanta MariaBrazil
  3. 3.Department of Microbiology and ParasitologyUniversidade Federal de Santa MariaSanta MariaBrazil
  4. 4.Department of Animal ScienceUniversidade do Estado de Santa CatarinaChapecóBrazil

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