Fish Physiology and Biochemistry

, Volume 44, Issue 3, pp 1011–1018 | Cite as

Effect of glyphosate-based herbicide on hematological and hemopoietic parameters in common carp (Cyprinus carpio L)

  • E. Kondera
  • B. Teodorczuk
  • K. Ługowska
  • M. Witeska
Article

Abstract

The effects of Roundup (glyphosate-based herbicide) on peripheral blood parameters and hematopoietic tissue cellular composition and activity in common carp juveniles were evaluated. The fish were exposed for 7 days at 0.0, 0.1, 0.5, or 5.0 mg/L of glyphosate. In fish exposed to Roundup, hematological alterations were observed; however, most of them were not directly related to the herbicide concentration. An increase in Ht and MCV, and decrease in Hb, MCH, and MCHC compared to the control were observed. Fish exposed to Roundup showed also a reduction in WBC and oxidative metabolic activity of phagocytes (NBT) compared to the control. The fish exposed to 0.1 and 5.0 mg/L showed increased glucose values, whereas in those subjected to 0.5 mg/L blood glucose concentration declined compared to the control. Cholesterol significantly increased at 0.1 mg/L and decreased at 5.0 mg/L. Analysis of head kidney hematopoietic tissue revealed that Roundup at concentrations 0.5 and 5.0 mg/L caused a significant increase in the rate of cell proliferation accompanied by an increase in frequency of early blast cells. No significant differences occurred in percentages of most cell lineages but the frequency of monocytoid, eosinophilic, and basophilic lineage cells significantly increased in the herbicide-exposed fish compared to the control. The obtained results revealed that sublethal concentrations of Roundup that may occur in polluted natural waters caused a slight anemic and significant immunosuppressive response in common carp juveniles. On the other hand, they indicate a considerable compensatory potential of carp hematopoietic system.

Keywords

Blood Fish Head kidney Roundup 

Notes

Acknowledgments

This work was financed from the science grant 387/14/S granted by the Ministry of Science and Higher Education.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • E. Kondera
    • 1
  • B. Teodorczuk
    • 1
  • K. Ługowska
    • 1
  • M. Witeska
    • 1
  1. 1.Department of Animal PhysiologyUniversity of Natural Sciences and HumanitiesSiedlcePoland

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