Characterization and expression analyses of somatolactin-α and -β genes in rare minnows (Gobiocypris rarus) following waterborne cadmium exposure
Using reverse transcription-polymerase chain reaction (RT-PCR) and RACE (rapid amplification of cDNA ends), somatolactin-α (rmSLα) and -β (rmSLβ) were identified from the pituitary gland of rare minnows (Gobiocypris rarus). The full-length cDNAs of these two genes were 1288 and 801 bp, encoding prepeptides of 250 and 228 amino acids residues, respectively. rmSLβ can be detected in the brain (including the pituitary), ovary, testis, and gill, while rmSLα was mainly expressed in the brain. On the other hand, rmSLα was expressed in all the fetal developmental stages; however, rmSLβ can just be detected in the stages since from 14 h post-fertilization (hpf). After exposure to acute waterborne cadmium (Cd), rmSLα was distinctly upregulated in juvenile rare minnows at all detected time points, from 24 to 96 h and 10 days, while rmSLβ was significantly altered only in 96 h or 10-day treatment groups. As for adults, acute Cd exposure caused alterations of both rmSLα and rmSLβ in the brain (containing the pituitary) at the 24 h; subchronic waterborne Cd treatment led to upregulation of rmSLα, while decrease of mSLβ in the brain. Alteration of rmSL transcripts following waterborne Cd exposure further confirmed the endocrine disruption of this heavy metal. Besides, exposure to as low as 5 μg/L Cd caused alteration of rmSLα, which suggested that rmSLα might be a potential biomarker for risk assessment of aquatic Cd.
KeywordsSomatolactin Rare minnow Waterborne cadmium exposure Subfunctionalization
This work was funded by the major program of science and technology commission foundation of Chongqing (cstc2014yykfc80001), China postdoctoral science foundation (2017M622949), and Chongqing postdoctoral science foundation special funded project (Xm2017071). We thank Dr. Qi Liu for helpful discussion, and we are grateful for the members in Zhang’s and Wang’s laboratory for fish raising and sample collection.
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