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Fish Physiology and Biochemistry

, Volume 44, Issue 3, pp 983–995 | Cite as

Characterization and expression analyses of somatolactin-α and -β genes in rare minnows (Gobiocypris rarus) following waterborne cadmium exposure

  • Xiao-Hong Liu
  • Bi-Wen Xie
  • Zhi-Jian Wang
  • Yao-Guang Zhang
Article

Abstract

Using reverse transcription-polymerase chain reaction (RT-PCR) and RACE (rapid amplification of cDNA ends), somatolactin-α (rmSLα) and -β (rmSLβ) were identified from the pituitary gland of rare minnows (Gobiocypris rarus). The full-length cDNAs of these two genes were 1288 and 801 bp, encoding prepeptides of 250 and 228 amino acids residues, respectively. rmSLβ can be detected in the brain (including the pituitary), ovary, testis, and gill, while rmSLα was mainly expressed in the brain. On the other hand, rmSLα was expressed in all the fetal developmental stages; however, rmSLβ can just be detected in the stages since from 14 h post-fertilization (hpf). After exposure to acute waterborne cadmium (Cd), rmSLα was distinctly upregulated in juvenile rare minnows at all detected time points, from 24 to 96 h and 10 days, while rmSLβ was significantly altered only in 96 h or 10-day treatment groups. As for adults, acute Cd exposure caused alterations of both rmSLα and rmSLβ in the brain (containing the pituitary) at the 24 h; subchronic waterborne Cd treatment led to upregulation of rmSLα, while decrease of mSLβ in the brain. Alteration of rmSL transcripts following waterborne Cd exposure further confirmed the endocrine disruption of this heavy metal. Besides, exposure to as low as 5 μg/L Cd caused alteration of rmSLα, which suggested that rmSLα might be a potential biomarker for risk assessment of aquatic Cd.

Keywords

Somatolactin Rare minnow Waterborne cadmium exposure Subfunctionalization 

Notes

Acknowledgments

This work was funded by the major program of science and technology commission foundation of Chongqing (cstc2014yykfc80001), China postdoctoral science foundation (2017M622949), and Chongqing postdoctoral science foundation special funded project (Xm2017071). We thank Dr. Qi Liu for helpful discussion, and we are grateful for the members in Zhang’s and Wang’s laboratory for fish raising and sample collection.

Supplementary material

10695_2018_487_MOESM1_ESM.eps (18.6 mb)
Figure S1 Three dimensional structure models of somatolactin-α (SLα) and (SLβ) genes predicted by Swiss-model Workspace. (EPS 19077 kb)
10695_2018_487_MOESM2_ESM.docx (50 kb)
ESM 2 (DOCX 48 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Xiao-Hong Liu
    • 1
  • Bi-Wen Xie
    • 2
  • Zhi-Jian Wang
    • 1
  • Yao-Guang Zhang
    • 1
  1. 1.Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of ChongqingSouthwest University School of Life SciencesChongqingChina
  2. 2.Conservation and Utilization of Fishes Resources in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan ProvinceNeijiang Normal University, School of Life ScienceNeijiangChina

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