Fish Physiology and Biochemistry

, Volume 44, Issue 3, pp 939–948 | Cite as

Effects of dietary inclusions of red beet and betaine on the acute stress response and muscle lipid peroxidation in rainbow trout

  • Julia Pinedo-Gil
  • Ana Belén Martín-Diana
  • Daniela Bertotto
  • Miguel Ángel Sanz-Calvo
  • Miguel Jover-Cerdá
  • Ana Tomás-Vidal
Article

Abstract

This study evaluates the effects of red beet (RB) and betaine on rainbow trout submitted to an acute stress challenge. A control diet was compared with four experimental diets in which red beet (14 and 28%) and betaine (0.9 and 1.63%) were incorporated in different concentrations according to a factorial design. Cortisol in plasma and fin, glucose and lactate plasma levels, and malondialdehide (MDA) in muscle were all measured before the stress challenge and 30 min and 6 and 12 h after the stress challenge as parameters to determine the diet effects. RB and betaine had no effect on cortisol, glucose, and MDA basal levels. However, lactate basal levels were significantly lower on fish fed with RB and betaine. Thirty minutes after the stress challenge, there was a significant increase in plasma and fin cortisol, glucose and lactate concentrations, although fish fed with diets containing RB and betaine showed significantly higher plasma cortisol values. MDA values of fish fed with 14% RB and 0.9% betaine were significantly higher than MDA values from fish fed with 28% RB and 1.63% betaine. After 6 and 12 h, plasma and fin cortisol and lactate levels recovered in a similar trend. Glucose plasma levels recovered in almost all groups 12 h after the stress. Also, MDA values recovered basal levels after 6 and 12 h. RB and betaine did not enhance the tolerance to the stress challenge compared to the control group, although the presence of these ingredients had no negative effect on any of the stress indicators.

Keywords

Red beet Betaine Rainbow trout Acute stress challenge 

Notes

Acknowledgements

This work has been co-funded with FEDER and INIA funds. Julia Pinedo has been granted with the FPI-INIA grant number 21 (call 2012, BOE-2012-13337).

Compliance with ethical standards

Ethical statement

The rainbow trout Oncorhynchus mykiss (Walbaum) study complied with the European Union Council 282 Directive 2010/63/UE, which lays down minimum standards for the protection of animals, and Spanish national legislation (Spanish Royal Decree 53/2013) protecting animals used in experimentation and for other scientific purposes and approved by Animal Ethics Committee of Agro-Technological Institute of Castilla y León (Spain).

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Julia Pinedo-Gil
    • 1
  • Ana Belén Martín-Diana
    • 2
  • Daniela Bertotto
    • 3
  • Miguel Ángel Sanz-Calvo
    • 2
  • Miguel Jover-Cerdá
    • 1
  • Ana Tomás-Vidal
    • 1
  1. 1.Research Group of Aquaculture and Biodiversity, Institute of Animal Science and TechnologyUniversitat Poliècnica de ValènciaValenciaSpain
  2. 2.Subdirection of Research and Technology, Agro-Technological Institute of Castilla y León, Consejería de Agricultura y GanaderíaValladolidSpain
  3. 3.Department of Comparative Biomedicine and Food ScienceUniversity of PadovaLegnaroItaly

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