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Fish Physiology and Biochemistry

, Volume 44, Issue 3, pp 747–768 | Cite as

Effects of Rhizoma Alismatis extract on biochemical indices and adipose gene expression in oleic acid-induced hepatocyte injury in Jian carp (Cyprinus carpio var. Jian)

  • Jinliang Du
  • Rui Jia
  • Li-ping Cao
  • Weidong Ding
  • Pao Xu
  • Guojun Yin
Article

Abstract

Fatty liver is an increasingly serious disease of fish in aquaculture. However, the mechanisms responsible for the occurrence of fatty liver remain unclear, and no effective methods for the prevention and treatment of this disease have yet been found. In the present study, we aimed to develop an in vitro model of hepatocyte injury using oleic acid as hepatotoxicant and evaluate the protective effects of Rhizoma Alismatis extract (RAE) in Jian carp using this model. Primary hepatocytes from Jian carp were isolated and purified and cultured in vitro. The result indicated that 0.4 mmol L−1 oleic acid and 48 h could be the optimal conditions to induce hepatocyte injury model in cultured hepatocytes. Hepatocytes were exposed to oleic acid, followed by the addition of RAE at 0, 1, 5, 10, 20, or 50 μg mL−1. The hepatocytes and supernatant were then analyzed. RAE suppressed oleic acid-induced elevations in aspartate aminotransferase, alanine aminotransferase, triglycerides, total cholesterol, lactate dehydrogenase, alkaline phosphatase, cholinesterase, malondialdehyde, γ-glutamyl transferase, cytochrome P450 1A, cytochrome P450 2E1, liver-type fatty acid binding protein, free fatty acid, fatty acid synthetase, and tumor necrosis factor-α (P < 0.01 or P < 0.05); reduced protein levels of cytochrome P450 1A, nuclear factor (NF)-κB p65, and NF-κB c-Rel; and inhibited cytochrome P4503A, NF-κB c-Rel, nuclear factor erythroid-related factor 2, peroxisome proliferator-activated receptor-α, and cytochrome P4501A mRNA levels. In conclusion, RAE exhibited a protective effect against hepatocyte injury in Jian carp. Further in vivo studies are needed to provide more evidence for the use of RAE as a hepatoprotective agent for the treatment of hepatocyte injury.

Keywords

Jian carp Hepatocyte Oleic acid Hepatocyte injury Rhizoma Alismatis extract 

Abbreviations

TC

Total cholesterol

ROS

Reactive oxygen species

TG

Triglycerides

RAE

Rhizoma Alismatis extract

FFA

Free fatty acid

CHE

Cholinesterase

GPT

Glutamate pyruvate transaminase

ALP

Alkaline phosphatase

GOT

Glutamate oxalate transaminase

γ-GT

γ-Glutamyl transferase

LDH

Lactate dehydrogenase

CYP 1A

Cytochrome P450 1A

MDA

Malondialdehyde

CYP 3A

Cytochrome P450 3A

PBS

Phosphate-buffered saline

CYP2E1

Cytochrome P4502E1

Nrf2

Nuclear factor erythroid-related factor 2

IgG

Immunoglobulin G

PPAR-α

Peroxisome proliferator-activated receptor-α

FFA

Free fatty acids

L-FABP

Liver-type fatty acid binding protein

FAS

Fatty acid synthase

ACoAC

Acetyl coenzyme A carboxylase

LPO

Lipid peroxide

FCS

Fetal calf serum

NF-κB p65

Nuclear factor (NF)-κB p65

NF-κB c-Rel

Nuclear factor (NF)-κB c-Rel

TNF-α

Tumor necrosis factor-α

NAFLD

Non-alcoholic fatty liver disease

BCA

Bicinchonininc acid

Notes

Acknowledgments

This work was supported by the Central Public-Interest Scientific Institution Basal Research Fund of China (2015JBFM01).

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiChina
  2. 2.International Joint Research Laboratory for Fish Immunopharmacology, Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiChina
  3. 3.Wuxi Fisheries CollegeNanjing Agricultural UniversityWuxiChina

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