Organ-specific effects of low-dose zinc pre-exposure on high-dose zinc induced mitochondrial dysfunction in large yellow croaker Pseudosciaena crocea
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The study was carried out to evaluate the effects of low-dose zinc (Zn) pre-exposure on survival rate, new Zn accumulation, and mitochondrial bioenergetics in the liver and spleen of large yellow croaker exposed to high-dose Zn. To the end, fish were pre-exposed to 0 and 2 mg L−1 Zn for 48 h and post-exposed to 0 and 12 mg L−1 Zn for 48 h. Twelve milligrams Zn per liter exposure alone reduced survival rate, but the effect did not appear in the 2 mg L−1 Zn pre-exposure groups. Two milligrams per liter Zn pre-exposure also ameliorated 12 mg Zn L−1 induced new Zn accumulation, reactive oxygen species (ROS) levels, and mitochondrial swelling in the liver. However, these effects did not appear in the spleen. In the liver, 2 mg L−1 Zn pre-exposure apparently relieved 12 mg L−1 Zn induced down-regulation of activities of ATP synthase (F-ATPase), succinate dehydrogenase (SDH), and malate dehydrogenase (MDH). The mRNA levels of these genes remained relatively stable in fish exposed to 12 mg L−1 Zn alone, but increased in fish exposed to 12 mg L−1 Zn with 2 mg L−1 Zn pre-treatment. In the spleen, 2 mg Zn L−1 pre-exposure did not mitigate the down-regulation of mRNA levels of genes and activities of relative enzymes induced by 12 mg L−1 Zn. In conclusion, our study demonstrated low-dose zinc pre-exposure ameliorated high-dose zinc induced mitochondrial dysfunction in the liver but not in the spleen of large yellow croaker, indicating an organ-specific effect.
KeywordsZinc Metal acclimation Mitochondrial energy metabolism Large yellow croaker
This work was supported by the Natural Science Foundation of Zhejiang Province (LY15C190009), National Natural Science Foundation of China (Grant No. 41606122), and Public Science and Technology Research Funds Projects of Ocean (201505025).
- Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254Google Scholar
- Dolci G, Vey L, Schuster A, Roversi K, Roversi K, Dias V, Pase C, Barcelos R, Antoniazzi C, Golombieski J (2014) Hypoxia acclimation protects against oxidative damage and changes in prolactin and somatolactin expression in silver catfish (Rhamdia quelen) exposed to manganese. Aquat Toxicol 157:175–185CrossRefPubMedGoogle Scholar
- James R, Pattu VJ, Devakiamma G, Sampath K (1991) Impact of sublethal levels of mercury on glycogen and selected respiratory enzymes in Heteropneustes fossilis and role of water hyacinth in reduction of Hg toxicity. Ind J Fish 38:249–252Google Scholar
- James R, Sampath K, Ponmani KP (1992) Effect of metal mixtures on activity of two respiratory enzymes and their recovery in Oreochromis mossambicus (Peters). Ind J Exp Biol 30:496–499Google Scholar
- Niu C, Rummer J, Brauner C, Schulte P (2008) Heat shock protein (Hsp70) induced by a mild heat shock slightly moderates plasma osmolarity increases upon salinity transfer in rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol C: Pharmacol Toxicol 148:437–444Google Scholar
- Pujolar J, Milan M, Marino I, Capoccioni F, Ciccotti E, Belpaire C, Covaci A, Malarvannan G, Patarnello T, Bargelloni L (2013) Detecting genome-wide gene transcription profiles associated with high pollution burden in the critically endangered European eel. Aquat Toxicol 132:157–164CrossRefPubMedGoogle Scholar
- Sampaio FG, de Lima Boijink C, Oba ET, dos Santos LRB, Kalinin AL, Rantin FT (2008) Antioxidant defenses and biochemical changes in pacu (Piaractus mesopotamicus) in response to single and combined copper and hypoxia exposure. Comp. Biochem. Physiol. C: Pharmacol. Toxicol. 147:43–51Google Scholar
- Wu C, Zhang D, Kan M, Lv Z, Zhu A, Su Y, Zhou D, Zhang J, Zhang Z, Xu M (2014) The draft genome of the large yellow croaker reveals well-developed innate immunity. Nat. Commun 5Google Scholar
- Zheng JL, Zeng L, Xu MY, Shen B, Wu CW (2016b) Different effects of low- and high-dose waterborne zinc on Zn accumulation, ROS levels, oxidative damage and antioxidant responses in the liver of large yellow croaker Pseudosciaena crocea. Fish Physiol Biochem 1–11Google Scholar