Fish Physiology and Biochemistry

, Volume 43, Issue 2, pp 653–661 | Cite as

Organ-specific effects of low-dose zinc pre-exposure on high-dose zinc induced mitochondrial dysfunction in large yellow croaker Pseudosciaena crocea

  • Jia-Lang Zheng
  • Shuang-Shuang Yuan
  • Bin Shen
  • Chang-Wen Wu


The study was carried out to evaluate the effects of low-dose zinc (Zn) pre-exposure on survival rate, new Zn accumulation, and mitochondrial bioenergetics in the liver and spleen of large yellow croaker exposed to high-dose Zn. To the end, fish were pre-exposed to 0 and 2 mg L−1 Zn for 48 h and post-exposed to 0 and 12 mg L−1 Zn for 48 h. Twelve milligrams Zn per liter exposure alone reduced survival rate, but the effect did not appear in the 2 mg L−1 Zn pre-exposure groups. Two milligrams per liter Zn pre-exposure also ameliorated 12 mg Zn L−1 induced new Zn accumulation, reactive oxygen species (ROS) levels, and mitochondrial swelling in the liver. However, these effects did not appear in the spleen. In the liver, 2 mg L−1 Zn pre-exposure apparently relieved 12 mg L−1 Zn induced down-regulation of activities of ATP synthase (F-ATPase), succinate dehydrogenase (SDH), and malate dehydrogenase (MDH). The mRNA levels of these genes remained relatively stable in fish exposed to 12 mg L−1 Zn alone, but increased in fish exposed to 12 mg L−1 Zn with 2 mg L−1 Zn pre-treatment. In the spleen, 2 mg Zn L−1 pre-exposure did not mitigate the down-regulation of mRNA levels of genes and activities of relative enzymes induced by 12 mg L−1 Zn. In conclusion, our study demonstrated low-dose zinc pre-exposure ameliorated high-dose zinc induced mitochondrial dysfunction in the liver but not in the spleen of large yellow croaker, indicating an organ-specific effect.


Zinc Metal acclimation Mitochondrial energy metabolism Large yellow croaker 



This work was supported by the Natural Science Foundation of Zhejiang Province (LY15C190009), National Natural Science Foundation of China (Grant No. 41606122), and Public Science and Technology Research Funds Projects of Ocean (201505025).


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Jia-Lang Zheng
    • 1
  • Shuang-Shuang Yuan
    • 1
  • Bin Shen
    • 1
  • Chang-Wen Wu
    • 1
  1. 1.National Engineering Research Center of Marine Facilities AquacultureZhejiang Ocean UniversityZhoushanPeople’s Republic of China

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