Fish Physiology and Biochemistry

, Volume 43, Issue 2, pp 517–525 | Cite as

Zebrafish phosvitin-derived peptide Pt5 inhibits melanogenesis via cAMP pathway

  • Yuan-Yuan Liu
  • Xiu-Rong Su
  • Shou-Sheng Liu
  • Shuang-Shuang Yang
  • Cheng-Yan Jiang
  • Yu ZhangEmail author
  • Shicui ZhangEmail author


Zebrafish phosvitin-derived peptide Pt5, consisting of the C-terminal 55 residues of phosvitin, has been shown to have an antimicrobial-immunomodulatory activity comparable to phosvitin. Here, we showed clearly that Pt5 had the capacity to inhibit tyrosinase (TYR) activity and melanin biosynthesis, and this inhibition was independent of cell proliferation and cytotoxic effects. Incubation of fluorescein isothiocyanate (FITC)-labeled Pt5 with B16F10 melanoma cells revealed that Pt5 was localized in the cytoplasm of the cells. In addition, Pt5 inhibited the expression of TYR, tyrosinase-related protein-1 (TRP-1), tyrosinase-related protein-2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16F10 melanoma cells and reduced the intracellular cyclic adenosine monophosphate (cAMP) concentration in the cells, but it did not affect the cellular contents of pERK1/2 and β-catenin, suggesting that Pt5 regulates melanin biosynthesis via cAMP signaling pathway rather than Wnt and MAPK pathways. Collectively, these data indicate that Pt5 has the potential to be used as a melanogenesis inhibitor in medical and cosmetic industry, a novel role ever reported.


Zebrafish Yolk protein Phosvitin-derived peptide Melanogenesis Tyrosinase 



Cyclic adenosine monophosphate


Extracellular signal-regulated kinase


Mitogen-activated protein kinase


Microphthalmia-associated transcription factor


Tyrosinase-related protein





This work was supported by the Natural Science Foundation of China (31372505) and in part by the Department of Science and Technology of Shandong Province (ZR2011CZ004).


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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Yuan-Yuan Liu
    • 1
    • 2
  • Xiu-Rong Su
    • 3
  • Shou-Sheng Liu
    • 2
  • Shuang-Shuang Yang
    • 2
  • Cheng-Yan Jiang
    • 2
    • 4
  • Yu Zhang
    • 2
    Email author
  • Shicui Zhang
    • 2
    Email author
  1. 1.Department of Medical LaboratoryShaoyang UniversityShaoyangChina
  2. 2.Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity, Department of Marine BiologyOcean University of ChinaQingdaoChina
  3. 3.School of Marine SciencesNingbo UniversityNingboChina
  4. 4.College of Life Science and TechnologyHonghe UniversityMengziChina

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