Potential contributions of heat shock proteins and related genes in sexual differentiation in yellow catfish (Pelteobagrus fulvidraco)
Sex determination and differentiation in ectotherms are very complicated affairs and usually affected by both genetic and environmental factors. Because of their temperature-sensitive expression, heat shock proteins (HSPs) are good candidates for temperature-dependent sex determination (TSD). Similar to most thermosensitive fish species, the male to female ratio increases with temperature in yellow catfish (Pelteobagrus fulvidraco). Yellow catfish is also a type of sexual size dimorphic fish, and the male individuals grow much faster than females of the same age. Therefore, research of sex differentiation in yellow catfish is important in aquiculture. In this attempt, a total of seven HSPs and related genes were identified from transcriptomes of yellow catfish by 454 pyrosequencing and Solexa sequencing that we did previously, including five genes with complete open reading frame (ORF). Phylogenetically, all these genes were compared with their counterparts from other vertebrates. All these genes were sex-biased expressed in gonads. Hspa5, Hip, and Cdc37 were expressed more highly in ovary than in testis, whereas Hsp90α, Hspb2, Hspb8, and Hspbp1 were expressed more highly in testis than in ovary. Additionally, the expression of these genes was assessed after 17α-methyltestosterone (MT) and 17α-ethinylestradiol (EE2) treatment, respectively. Our result showed that working as co-chaperones, these HSPs and related genes may regulate sex steroid receptor activities to influence gonad development in yellow catfish. Our work would help in the understanding of the mechanism of sexual differentiation in teleosts.
KeywordsHeat shock proteins Pelteobagrus fulvidraco Sex differentiation Sex steroid
Authors thank Allison Malick for her kind reading of the manuscript. This work was funded by the National Natural Science Foundation of China (31301931) and the Fundamental Research Funds for the Central Universities (2013PY068, 2014 BC007). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Compliance with ethical standards
Experiments were performed in accordance with the guiding principles for biomedical research involving animals of Ethics and Animal Welfare Committee of College Fisheries, Huazhong Agricultural University and the committee approved this study. Euthanasia (Barbitone sodium immersion) was taken to avoid suffering when killing the fish for sampling.
Conflict of interest
The authors declare that they have no conflict of interest.
- Baroiller JF, Clota F (1998) Interactions between temperature effects and genotype on Oreochromis niloticus sex determination. J Exp Zool 281:507Google Scholar
- Dimitris GS, Ioannis L, Constantinos CM, Maroudio K (2012) Temperature during early life determines sex in zebrafish, Danio rerio (Hamilton, 1822). J Biol Res-Thessalon 17:68–73Google Scholar
- Kamiya T, Kai W, Tasumi S, Oka A, Matsunaga T, Mizuno N, Fujita M, Suetake H, Suzuki S, Hosoya S, Tohari S, Brenner S, Miyadai T, Venkatesh B, Suzuki Y, Kikuchi K (2012) A trans-species missense SNP in Amhr2 is associated with sex determination in the tiger pufferfish, Takifugu rubripes (fugu). PLoS Genet 8(7):e1002798CrossRefPubMedPubMedCentralGoogle Scholar
- Nanda I, Kondo M, Hornung U, Asakawa S, Winkler C, Shimizu A, Shan Z, Haaf T, Shimizu N, Shima A, Schmid M, Schartl M (2002) A duplicated copy of DMRT1 in the sex-determining region of the Y chromosome of the medaka, Oryzias latipes. Proc Natl Acad Sci U S A 99(18):11778–11783CrossRefPubMedPubMedCentralGoogle Scholar
- Pitetti JL, Calvel P, Romero Y, Conne B, Truong V, Papaioannou MD, Schaad O, Docquier M, Herrera PL, Wilhelm D, Nef S (2013) Insulin and IGF1 receptors are essential for XX and XY gonadal differentiation and adrenal development in mice. PLoS Genet 9(1):e1003160CrossRefPubMedPubMedCentralGoogle Scholar
- Widlak W, Vydra N, Malusecka E, Dudaladava V, Winiarski B, Scieglinska D, Widlak P (2007) Heat shock transcription factor 1 down-regulates spermatocyte-specific 70 kDa heat shock protein expression prior to the induction of apoptosis in mouse testes. Genes Cells 12(4):487–499CrossRefPubMedGoogle Scholar
- You X, Deng M, Liu Q, Qin Y, He X (2016) Gonadal differentiation and effects of temperature on sex determination in yellow catfish, Pseudobagrus fulvidraco Richardson. J Huazhong Agri Univ 35(1):106–113Google Scholar