Different effects of blue and red light-emitting diodes on antioxidant responses in the liver and ovary of zebrafish Danio rerio
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The present study assessed the effects of a white fluorescent bulb (the control) and two different light-emitting diodes (blue LEDs, LDB; red, LDR) on growth, morphology, and oxidative stress in the liver and ovary of zebrafish for 5 weeks. Growth maintained relatively constant under LDB condition, but was reduced under LDR condition. In the liver, hepatosomatic index (HSI) and protein carbonylation (PC) increased under LDR condition, whereas lipid peroxidation (LPO) declined and HSI remained unchanged under LDB condition. The decrease in oxidative damage by LDB could be attributed to the up-regulated levels of mRNA, protein, and activity of Cu/Zn-SOD and CAT. A failure to activate the activity of both enzymes may result in the enhanced PC levels under LDR condition, though both genes were up-regulated at transcriptional and translational levels. In the ovary, although gonadosomatic index sharply increased under LDR condition, LPO and PC dramatically accumulated. The increase in oxidative damage by LDR might result from the down-regulated levels of protein and activity of Cu/Zn-SOD and CAT, though both genes were up-regulated at a transcriptional level. Furthermore, a sharp increase in expression of transcription factor Nrf2 that targets antioxidant genes was observed in the liver but not in the ovary under LDB and LDR conditions. In conclusion, our data demonstrated a positive effect of LDB and negative effect of LDR on fish antioxidant defenses, emphasizing the potentials of LDB as an effective light source in fish farming.
KeywordsReproductive toxicity Oxidative stress Light spectrum Fish
This work was supported by Scientific Research Foundation of Zhejiang Ocean University (22115010215) and Public Science and Technology Research Funds Projects of Ocean (No. 201505025).
- Beutler E (1982) Catalase. Red cell metabolism, a manual of biochemical methods, pp 105–106Google Scholar
- Brusle J, Anadon G (1996) The structure and function of fish liver. Fish Morphol 77–93Google Scholar
- Lythgoe JN (1979) Ecology of vision. Clarendon Press; Oxford University Press, OxfordGoogle Scholar
- Shi X, Zhou B (2010) The role of Nrf2 and MAPK pathways in PFOS-induced oxidative stress in zebrafish embryos. Toxicol Sci kfq066Google Scholar
- Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:research0034Google Scholar
- Wu P, Jiang WD, Liu Y, Chen GF, Jiang J, Li SH, Feng L, Zhou XQ (2014) Effect of choline on antioxidant defenses and gene expressions of Nrf2 signaling molecule in the spleen and head kidney of juvenile Jian carp (Cyprinus carpio var. Jian). Fish Shellfish Immunol 38:374–382CrossRefPubMedGoogle Scholar