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Coordinated expression and regulation of deiodinases and thyroid hormone receptors during metamorphosis in the Japanese flounder (Paralichthys olivaceus)

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Abstract

In vertebrates, thyroid hormone receptors (TRs) and deiodinases are essential for developmental events driven by the thyroid hormones (THs). However, the significance of deiodinases during the metamorphosis of the Japanese flounder (Paralichthys olivaceus) remains unclear. Moreover, regulation and response of the TRs and deiodinases to THs in this fish are poorly understood. Therefore, we detected the expression patterns of THs, deiodinases, and TRs in drug-treated larvae and untreated larvae of P. olivaceus by using enzyme-linked immunosorbent assay and quantitative real-time PCR during P. olivaceus metamorphosis. To further understand the roles of these elements, a rescue assay was performed. Our results show the importance of THs, TRs, and deiodinases in flatfish metamorphosis. Our results also confirm that D1 and D2 activate THs and D3 plays the opposite and complementary role. Moreover, we demonstrated that both TRα and TRβ have important but different roles during P. olivaceus metamorphosis.

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Acknowledgments

The authors thank Prof. Haijin Liu (Chinese Academy of Fishery Sciences) for providing the experimental fish. This work was supported by the National Natural Science Foundation of China (No. 31172392).

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    1. Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, 999, Hu-Cheng-Huan Road, Lingang New City, Shanghai, 201306, China

      Jie Yu, Yuanshuai Fu & Zhiyi Shi

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    1. Jie Yu
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    Correspondence to Zhiyi Shi.

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    Jie Yu and Yuanshuai Fu have contributed equally to this article.

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    Yu, J., Fu, Y. & Shi, Z. Coordinated expression and regulation of deiodinases and thyroid hormone receptors during metamorphosis in the Japanese flounder (Paralichthys olivaceus). Fish Physiol Biochem 43, 321–336 (2017). https://doi.org/10.1007/s10695-016-0289-0

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