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Fish Physiology and Biochemistry

, Volume 43, Issue 2, pp 321–336 | Cite as

Coordinated expression and regulation of deiodinases and thyroid hormone receptors during metamorphosis in the Japanese flounder (Paralichthys olivaceus)

  • Jie Yu
  • Yuanshuai Fu
  • Zhiyi Shi
Article

Abstract

In vertebrates, thyroid hormone receptors (TRs) and deiodinases are essential for developmental events driven by the thyroid hormones (THs). However, the significance of deiodinases during the metamorphosis of the Japanese flounder (Paralichthys olivaceus) remains unclear. Moreover, regulation and response of the TRs and deiodinases to THs in this fish are poorly understood. Therefore, we detected the expression patterns of THs, deiodinases, and TRs in drug-treated larvae and untreated larvae of P. olivaceus by using enzyme-linked immunosorbent assay and quantitative real-time PCR during P. olivaceus metamorphosis. To further understand the roles of these elements, a rescue assay was performed. Our results show the importance of THs, TRs, and deiodinases in flatfish metamorphosis. Our results also confirm that D1 and D2 activate THs and D3 plays the opposite and complementary role. Moreover, we demonstrated that both TRα and TRβ have important but different roles during P. olivaceus metamorphosis.

Keywords

Japanese flounder (Paralichthys olivaceusMetamorphosis Deiodinases Thyroid hormone receptors 

Notes

Acknowledgments

The authors thank Prof. Haijin Liu (Chinese Academy of Fishery Sciences) for providing the experimental fish. This work was supported by the National Natural Science Foundation of China (No. 31172392).

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© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Key Laboratory of Genetic Resources for Freshwater Aquaculture and FisheriesShanghai Ocean UniversityShanghaiChina

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