Aging asymmetry: systematic survey of changes in age-related biomarkers in the annual fish Nothobranchius guentheri
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Aging asymmetry is the observation that different tissues age in different ways and at different rates. This has not been assessed in a single organism using multiple biomarkers of aging. Here we clearly demonstrated that the levels of protein oxidation and lipid peroxidation as well as CAT, SOD and GPX activities all showed a tissue-dependent change with advancing age; and DNA repair ability, as revealed by the expression of ercc1 and its protein levels, also exhibited a tissue-specific variation with age. We also found that protein oxidation and lipid peroxidation levels remained relatively stable in the liver, intestine, skin and testis as well as in the brain, eye and heart of young, adult and aged fishes; SOD and GPX activities displayed little variation in the intestine, eye and skin as well as in the brain and skin of young, adult and aged fishes; and low and stable expression of ercc1 was observed in the spleen, eye and heart of young, adult and aged fishes. Collectively, these results indicate that aging is tissue specific and asymmetric in N. guentheri. The observation of aging asymmetry may have practical implications for the application of non-intrusion intervention approaches to prolong lifespan.
KeywordsAging asymmetry Age-related markers Annual fish Nothobranchius Reactive oxygen species ercc1
This work was supported by the grants of Natural Science Foundation of China (31372505; U1401211). No competing financial interests exist. S.C. Zhang designed the research and wrote the manuscript; Y. Dong performed the research, analyzed the data and wrote the manuscript; P.F. Cui and Z.J. Li participated in the research.
- Facista A, Nguyen H, Lewis C, Prasad AR, Ramsey L, Zaitlin B, Nfonsam V, Krouse RS, Bernstein H, Payne CM, Stern S, Oatman N, Banerjee B, Bernstein C (2012) Deficient expression of DNA repair enzymes in early progression to sporadic colon cancer. Genome Integr 3:3. doi: 10.1186/2041-9414-3-3 CrossRefPubMedPubMedCentralGoogle Scholar
- Koutsouleris N, Davatzikos C, Borgwardt S, Gaser C, Bottlender R, Frodl T, Falkai P, Riecher-Rössler A, Möller HJ, Reiser M, Pantelis C, Meisenzahl E (2014) Accelerated brain aging in schizophrenia and beyond: a neuroanatomical marker of psychiatric disorders. Schizophr Bull 40:1140–1153. doi: 10.1093/schbul/sbt142 CrossRefPubMedGoogle Scholar
- Latimer CS, Searcy JL, Bridges MT, Brewer LD, Popović J, Blalock EM, Landfield PW, Thibault O, Porter NM (2011) Reversal of glial and neurovascular markers of unhealthy brain aging by exercise in middle-aged female mice. PLoS One 6:e26812. doi: 10.1371/journal.pone.0026812 CrossRefPubMedPubMedCentralGoogle Scholar
- Wang G, Zhang S, Wang Z (2009) Responses of alternative complement expression to challenge with different combinations of Vibrio anguillarum, Escherichia coli and Staphylococcus aureus: evidence for specific immune priming in amphioxus Branchiostoma belcheri. Fish Shellfish Immunol 26:33–39. doi: 10.1016/j.fsi.2008.09.018 CrossRefPubMedGoogle Scholar
- Wu JJ, Liu J, Chen EB, Wang JJ, Cao L, Narayan N, Fergusson MM, Rovira II, Allen M, Springer DA, Lago CU, Zhang S, DuBois W, Ward T, deCabo R, Gavrilova O, Mock B, Finkel T (2013) Increased mammalian lifespan and a segmental and tissue-specific slowing of aging after genetic reduction of mTOR expression. Cell Rep 4:913–920. doi: 10.1016/j.celrep.2013.07.030 CrossRefPubMedPubMedCentralGoogle Scholar