Fish Physiology and Biochemistry

, Volume 43, Issue 2, pp 309–319 | Cite as

Aging asymmetry: systematic survey of changes in age-related biomarkers in the annual fish Nothobranchius guentheri

  • Yuan Dong
  • Pengfei Cui
  • Zhijian Li
  • Shicui Zhang


Aging asymmetry is the observation that different tissues age in different ways and at different rates. This has not been assessed in a single organism using multiple biomarkers of aging. Here we clearly demonstrated that the levels of protein oxidation and lipid peroxidation as well as CAT, SOD and GPX activities all showed a tissue-dependent change with advancing age; and DNA repair ability, as revealed by the expression of ercc1 and its protein levels, also exhibited a tissue-specific variation with age. We also found that protein oxidation and lipid peroxidation levels remained relatively stable in the liver, intestine, skin and testis as well as in the brain, eye and heart of young, adult and aged fishes; SOD and GPX activities displayed little variation in the intestine, eye and skin as well as in the brain and skin of young, adult and aged fishes; and low and stable expression of ercc1 was observed in the spleen, eye and heart of young, adult and aged fishes. Collectively, these results indicate that aging is tissue specific and asymmetric in N. guentheri. The observation of aging asymmetry may have practical implications for the application of non-intrusion intervention approaches to prolong lifespan.


Aging asymmetry Age-related markers Annual fish Nothobranchius Reactive oxygen species ercc1 



This work was supported by the grants of Natural Science Foundation of China (31372505; U1401211). No competing financial interests exist. S.C. Zhang designed the research and wrote the manuscript; Y. Dong performed the research, analyzed the data and wrote the manuscript; P.F. Cui and Z.J. Li participated in the research.

Supplementary material

10695_2016_288_MOESM1_ESM.doc (1.2 mb)
Supplementary material 1 (DOC 1243 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Yuan Dong
    • 1
  • Pengfei Cui
    • 1
  • Zhijian Li
    • 1
  • Shicui Zhang
    • 1
  1. 1.Laboratory for Evolution and Development, Department of Marine Biology, Institute of Evolution and Marine BiodiversityOcean University of ChinaQingdaoChina

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