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Fish Physiology and Biochemistry

, Volume 42, Issue 2, pp 659–671 | Cite as

Chemokine receptor CXCR3 in turbot (Scophthalmus maximus): cloning, characterization and its responses to lipopolysaccharide

  • Yadong Chen
  • Shuhong Zhou
  • Zhiqiang Jiang
  • Xiuli Wang
  • Yang Liu
Article

Abstract

Chemokine (C-X-C motif) receptor 3, a member of the G protein-coupled receptors superfamily, regulates the responses of many immune responses. In this experiment, we cloned and characterized the cDNA of CXCR3 in Scophthalmus maximus (turbot). A 5′-UTR of 216-bp, a 259-bp 3′-UTR with a poly (A) tail and a 1089-bp CDS encoding 362 amino acids form the cDNA of CXCR3, which is 1564-bp long. Phylogenetic analyses indicated that turbot CXCR3 shared a high similarity with other CXCR3s and shared more similarity with CXCR5 than the other subfamilies of chemokines. The CXCR3 protein in turbot showed the highest similarity with the CXCR3b from rainbow trout (44.5 %), which indicated that this CXCR3 gene/protein may be a CXCR3b isoform. Quantitative real-time PCR analysis showed that CXCR3 transcripts were constitutively expressed in all the tissues of the non-injected turbot used in this study, with the highest expression occurring in blood. Several immune-related tissues of fish, such as the spleen, head kidney, liver and blood, tissues, which were abundant of lymphocyte, were investigated in this study. CXCR3 gene was expressed at the highest level in blood than the other tested tissues. The injection experiment suggested that the CXCR3 expression level after LPS injection was significantly up-regulated in all immune-related tissues in turbot. These results improve our understanding of the functions of CXCR3 in the turbot immune response.

Keywords

Chemokine receptor Immune response Lipopolysaccharide Scophthalmus maximus 

Notes

Acknowledgments

This work was supported by Grants from State 863 High-Technology R&D Project (2012AA10A408).

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Yadong Chen
    • 1
    • 2
  • Shuhong Zhou
    • 1
  • Zhiqiang Jiang
    • 1
  • Xiuli Wang
    • 1
  • Yang Liu
    • 1
  1. 1.Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of AgricultureDalian Ocean UniversityDalianPeople’s Republic of China
  2. 2.Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoPeople’s Republic of China

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