Activity of the hypothalamus–pituitary–interrenal axis (HPI axis) and immune response in carp lines with different susceptibility to disease
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The stress response transmitted by the HPA axis is one of the best examples of neuroendocrine–immune interactions that are critical for survival. Analogous to the situation in mammals, the stress response in fish is characterized by the activation of the hypothalamo–pituitary–interrenal axis (HPI). Effects of cortisol on the fish immune system comply with findings in mammals and suggest that the differences in sensitivity to stress will influence the immune response and as a consequence of survival. Therefore, we studied the stress response and its immunity-related effects in four different carp lines (R3, R3xR8, K and R2) that display a differential pathogen susceptibility. Previous studies indicate that R3xR8 and R3 carp are susceptible to bacterial and parasite infection, while R2 and K are relatively resistant to infection. Interestingly, the most striking effect of stress on leukocyte composition and activity was observed in the pathogen-resistant K carp, even though no robust changes in gene expression of stress-involved factors were observed. In contrast, R3 carp showed no spectacular stress-induced changes in their immunological parameters with concurrent significant activation of the HPI axis. Upon stress, the R3 carp showed up-regulation of crf, pomc and gr2 gene expression in the hypothalamus. Furthermore in R3 carp, at all levels of the HPI axis, stress induced the highest up-regulation of il-1β gene expression. Although we are aware of the complexity of the interactions between stress and pathogen susceptibility and of the risk of interpretation based on correlations, it is noteworthy that the fish more susceptible to infection also exhibited the highest response to stress.
KeywordsCarp lines Stress response Leukocyte activity Pathogen susceptibility Cortisol
This work was supported by the Polish National Science Center (Grant No. N N308 572039) and by the Wageningen Institute of Animal Sciences. We are grateful to Marleen Scheer for her technical assistance.
- Ainsworth AJ, Dexiang C, Waterstrat PR, Greenway T (1991) Effect of temperature on the immune system of channel catfish (Ictalurus punctatus)—I. Leucocyte distribution and phagocyte function in the anterior kidney at 10 degrees C. Comp Biochem Physiol, A: Comp Physiol 100:907–912CrossRefGoogle Scholar
- Bekh V, Irnazarow I, Onara D, Rakus K, Jurecka P, Pilarczyk A (2004) Studies of experimental infection with Aeromonas hydrophila in common carp (Cyprinus carpio L.). In: Siwicki AK, Antychowicz J, Szweda W (eds) Ochrona zdrowia ryb—aktualne problemy. Wyd. IRS., Olsztyn, pp 143–150 (in Polish) Google Scholar
- Campbell TW, Ellis CK (2007) Avian and exotic animal hematology and cytology. Blackwell Publishing, AmesGoogle Scholar
- Elenkov IJ, Chrousos GP (2006) Stress system—organization, physiology and immunoregulation. Neuro Immuno Modul 13:257–267Google Scholar
- Ellis AE (1990) Lysozyme assays. In: Stolen JS, Fletcher TC, Anderson DP, Roberson BS, van Muiswinkel WB (eds) Techniques in fish immunology. SOS Publications, New Jersey, p 101e3Google Scholar
- Huising MO, Metz JR, van Schooten C, Taverne-Thiele AJ, Hermsen T, Verburg-van Kemenade BM, Flik G (2004a) Structural characterisation of a cyprinid (Cyprinus carpio L.) CRH, CRH-BP and CRH-R1, and the role of these proteins in the acute stress response. J Mol Endocrinol 32:627–648CrossRefPubMedGoogle Scholar
- Huising MO, van der Aa LM, Metz JR, de Fátima Mazon A, Verburg-van Kemenade BM, Flik G (2007) Corticotropin-releasing factor (CRF) and CRF-binding protein expression in and release from the head kidney of common carp: evolutionary conservation of the adrenal CRF system. J Endocrinol 193:349–357CrossRefPubMedGoogle Scholar
- Rakus KL, Irnazarow I, Adamek M, Palmeira L, Kawana Y, Hirono I, Kondo H, Matras M, Steinhagen D, Flasz B, Brogden G, Vanderplasschen A, Aoki T (2012) Gene expression analysis of common carp (Cyprinus carpio L.) lines during cyprinid herpesvirus 3 infection yields insights into differential immune responses. Dev Comp Immunol 37:65–76CrossRefPubMedGoogle Scholar
- Stolte EH, Chadzinska M, Przybylska D, Flik G, Savelkoul HF, Verburg-van Kemenade BM (2009) The immune response differentially regulates Hsp70 and glucocorticoid receptor expression in vitro and in vivo in common carp (Cyprinus carpio L.). Fish Shellfish Immunol 27:9–16Google Scholar