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Protection of common carp (Cyprinus carpio L.) spermatozoa motility under oxidative stress by antioxidants and seminal plasma

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Abstract

The protective influence of seminal plasma and the antioxidants catalase (CAT), superoxide dismutase (SOD), and glutathione (GTH) on quality parameters, oxidative stress indices, and antioxidant activity was studied in common carp (Cyprinus carpio) spermatozoa exposed to the xanthine–xanthine oxidase (X–XO) system. Fish spermatozoa were incubated for 5 and 20 min at 4 °C with X–XO concentrations of 1 mM X–0.1 U/mL, 0.6 mM X–0.05 U/mL, 0.3 mM X–0.025 U/mL, and 0.1 mM X–0.0125 U/mL. A dose-dependent reduction in spermatozoa motility and velocity was observed at concentrations of 0.1 mM X–0.0125 U/mL to 1 mM X–0.1 U/mL XO. Increase in spermatozoa motility parameters was recorded following treatment with antioxidants and seminal plasma. The level of the oxidative stress indices lipid peroxidation (LPO) and carbonyl derivatives of proteins (CP) was significantly reduced after addition of CAT, SOD, or GTH along with seminal plasma. Significant differences in SOD, glutathione reductase, and glutathione peroxidase activity were seen in spermatozoa incubated with, compared to that without, seminal plasma at all studied X–XO concentrations. The data demonstrated that CAT, SOD, or GTH in combination with SP can reduce reactive oxygen species stress in fish spermatozoa and improve spermatozoa quality.

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Abbreviations

X–XO:

Xanthine–xanthine oxidase

LPO:

Lipid peroxidation

CP:

Carbonyl proteins

TBARS:

Thiobarbituric acid-reactive substances

SOD:

Superoxide dismutase

GR:

Glutathione reductase

GPx:

Glutathione peroxidase

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Acknowledgments

Support for this research was provided by projects: CENAKVA CZ.1.05/2.1.00/01.0024, strengthening of excellence of scientific teams in USB FFPW CZ.1.07/2.3.00/20.0024, GAJU 114/2013/Z and GACR P502/11/0090 and No. GACR P502/12/1973. The results of the project LO1205 were obtained with a financial support from the MEYS of the CR under the NPU I program. The Lucidus Consultancy, UK, is gratefully acknowledged for English correction and suggestions.

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Authors and Affiliations

  1. Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, University of South Bohemia in Ceske Budejovice, Zátiší 728/II, 389 25, Vodňany, Czech Republic

    A. Shaliutina-Kolešová, I. Gazo, J. Cosson & O. Linhart

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  1. A. Shaliutina-Kolešová
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  2. I. Gazo
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  3. J. Cosson
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Correspondence to A. Shaliutina-Kolešová.

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Shaliutina-Kolešová, A., Gazo, I., Cosson, J. et al. Protection of common carp (Cyprinus carpio L.) spermatozoa motility under oxidative stress by antioxidants and seminal plasma. Fish Physiol Biochem 40, 1771–1781 (2014). https://doi.org/10.1007/s10695-014-9966-z

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