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Fish Physiology and Biochemistry

, Volume 40, Issue 6, pp 1683–1691 | Cite as

Stocking density and Piscirickettsia salmonis infection effect on Patagonian blennie (Eleginops maclovinus, Cuvier 1830) skeletal muscle intermediate metabolism

  • L. Vargas-Chacoff
  • E. Ortíz
  • R. Oyarzún
  • D. Martínez
  • E. Saavedra
  • R. Sá
  • V. Olavarría
  • D. Nualart
  • A. Yáñez
  • C. Bertrán
  • J. M. Mancera
Article

Abstract

The need to expand aquaculture production has led to other fish to be considered as potential species for culture, such as the sub-Antarctic notothenioid Eleginops maclovinus (Valenciennes, 1830). The aim of this study was to determine the cumulative effect of density and pathogen infection by protein extract of Piscirickettsia salmonis on skeletal muscle metabolism. In a first experiment, specimens were submitted to three different stocking densities: (1) 3.1 kg m−3, (2) 15 kg m−3 and (3) 60 kg m−3, for a period of 10 days. In a second experiment, metabolic changes caused by an infection of P. salmonis protein extract (a single injection of 0.5 μL P. salmonis protein extract g body weight−1 was inoculated in the fish) and its combined effect with stocking density was assessed during a period of 10 days. This study concludes that stress caused by high stocking density led to the reorganization of some metabolic routes to fulfill skeletal muscle energy needs. Furthermore, infection response by pathogen P. salmonis differed when stocking density increased, suggesting an increase of energy needs with density in skeletal muscle of infected fish.

Keywords

E. maclovinus Metabolism P. salmonis Skeletal muscle Stress 

Abbreviations

GPT

Alanine aminotransferase (EC 2.6.1.2)

GOT

Aspartate aminotransferase (EC 2.6.1.1)

EDTA

Ethylenediamine tetraacetic acid

GDH

Glutamate dehydrogenase (EC 1.4.1.2)

G3PDH

Glycerol 3-phosphate dehydrogenase (EC 1.1.1.8)

LDH-O

Lactate dehydrogenase oxidase (EC 1.1.1.27)

Notes

Acknowledgments

This study was carried out in the framework of FONDECYT Projects 11080168 and 1110235 and FONDAP-INCAR, No. 15110027. We thank Dr. Lafayette Eaton for his help checking this manuscript and the Dirección de Investigación of the Universidad Austral de Chile (DID).

Conflict of interest

The authors declare that there are no conflicts of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • L. Vargas-Chacoff
    • 1
  • E. Ortíz
    • 1
  • R. Oyarzún
    • 1
  • D. Martínez
    • 1
  • E. Saavedra
    • 1
  • R. Sá
    • 2
  • V. Olavarría
    • 3
  • D. Nualart
    • 3
  • A. Yáñez
    • 3
  • C. Bertrán
    • 1
  • J. M. Mancera
    • 4
  1. 1.Instituto de Ciencias Marinas y LimnológicasUniversidad Austral de ChileValdiviaChile
  2. 2.Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería ForestalPontificia Universidad Católica de ChileMaculChile
  3. 3.Instituto de Bioquímica y Microbiología, Interdisciplinary Center for Aquaculture Research (FONDAP-INCAR)Universidad Austral de ChileValdiviaChile
  4. 4.Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEI MAR)Universidad de CádizPuerto RealSpain

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