Fish Physiology and Biochemistry

, Volume 40, Issue 5, pp 1437–1445 | Cite as

Oxidative damage repair by glutamine in fish enterocytes

  • Kai Hu
  • Lin Feng
  • Weidan Jiang
  • Yang Liu
  • Jun Jiang
  • Shuhong Li
  • Xiaoqiu Zhou


Fish intestine is very sensitive to oxidative damage. Repair of damaged enterocytes may be involved to restore normal function of fish intestine. However, studies of fish enterocyte repair are scarce. The present study aimed to investigate the potential repair role of glutamine after a H2O2 challenge. In this study, fish enterocytes were post-treated with graded levels of glutamine (0, 4, 8, 12 and 20 mM of glutamine) after expose to 100 μM H2O2. The basal control cells were kept in the glutamine-free minimum essential medium only. Results showed that the H2O2-induced decreases in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide optical density, alkaline phosphatase and Na+, K+-ATPase activities were completely restored by subsequent glutamine treatments. In addition, cellular injury (lactate dehydrogenase), lipid peroxidation (malondialdehyde) and protein oxidation (protein carbonyls) caused by H2O2 were reversed by subsequent glutamine treatments. Furthermore, the H2O2-induced decreases in glutathione contents, glutathione reductase, superoxide dismutase and glutathione peroxidase activities were completely restored by subsequent glutamine treatments. In summary, the present study indicated that glutamine improved the repair activity in fish enterocytes after challenge with H2O2.


Glutamine Oxidative damage Repair Glutathione Antioxidant enzyme Fish enterocyte 



This research was financially supported by the National Science Foundation of China (30771671, 30871926), the National Department Public Benefit Research Foundation (Agriculture) of China (201003020) and the Science and Technology Support Program of Sichuan Province (2011NZ0071). The authors wish to thank the personnel of these teams for their kind assistance.


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Kai Hu
    • 1
    • 2
    • 3
  • Lin Feng
    • 1
    • 2
    • 3
  • Weidan Jiang
    • 1
    • 2
    • 3
  • Yang Liu
    • 1
    • 3
  • Jun Jiang
    • 1
    • 3
  • Shuhong Li
    • 1
  • Xiaoqiu Zhou
    • 1
    • 2
    • 3
  1. 1.Animal Nutrition InstituteSichuan Agricultural UniversityChengduChina
  2. 2.Fish Nutrition and Safety in Production Sichuan University Key LaboratorySichuan Agricultural UniversityChengduChina
  3. 3.Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of EducationSichuan Agricultural UniversityChengduChina

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