Fish Physiology and Biochemistry

, Volume 40, Issue 4, pp 1281–1288 | Cite as

Cloning and expression of prostaglandin E2 receptor subtype 1 (ep 1 ) in Bostrichthys sinensis

  • Xiao Jian Lai
  • Wan Shu Hong
  • Fang Liu
  • Yu Ting Zhang
  • Shi Xi Chen


Our previous studies suggested that prostaglandin E2 (PGE2) is a putative sex pheromone in Chinese black sleeper Bostrichthys sinensis, a fish species that inhabits intertidal zones and mates and spawns inside a muddy burrow. We found immunoreactivities of PGE2 receptor subtypes (Ep1–3) expressed in the olfactory sac, but only Ep1 presented higher density of immunoreactivity in mature fish than that in immature fish in both sexes. To gain a better understanding of the underlying molecular mechanism for the detection of PGE2 in the olfactory system, we cloned an ep 1 cDNA from the adult olfactory sac. The open-reading frame of the ep 1 consisted of 1,134-bp nucleotides that encoded a 378-amino acid-long protein with a seven-transmembrane domain, typical for the G protein-coupled receptors superfamily. Expression of ep 1 mRNA was observed in all tissues examined, with higher levels obtained in the olfactory sacs and testes. The expression of ep 1 mRNA in the olfactory sacs and gonads was significantly higher in both sexes of mature fish than in those of immature ones. Taken together, our results suggested that Ep1, which is highly expressed in the olfactory sacs and gonads of mature fish, is important for the control of reproduction and may be involved in PGE2-initiated spawning behavior in B. sinensis.


Bostrichthys sinensis PGE2 Ep1 Sex pheromone Olfactory system 



This work was supported by the National Natural Science Foundation of China (No. 41276129, 40776080), the fund for Doctor Station of the Ministry of China (No. 20120121110029) and the Major Project of Agricultural Science and Technology of Fujian Province (2012N5011). Professors J. Hodgkiss and Y. Zhu are thanked for their help with English language of the manuscript.

Supplementary material

10695_2014_9923_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 49 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Xiao Jian Lai
    • 2
  • Wan Shu Hong
    • 1
    • 3
  • Fang Liu
    • 3
  • Yu Ting Zhang
    • 3
  • Shi Xi Chen
    • 1
    • 3
  1. 1.State Key Laboratory of Marine Environmental ScienceXiamen UniversityXiamenPeople’s Republic of China
  2. 2.College of FisheriesJimei UniversityXiamenPeople’s Republic of China
  3. 3.College of Ocean and Earth SciencesXiamen UniversityXiamenPeople’s Republic of China

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