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Fish Physiology and Biochemistry

, Volume 41, Issue 1, pp 107–117 | Cite as

Characterization of incomplete vitellogenin (VgC) in the Indian freshwater murrel, Channa punctatus (Bloch)

  • S. Pipil
  • V. S. Rawat
  • L. Sharma
  • N. Sehgal
Article

Abstract

A novel incomplete vitellogenin (VgC) was purified from the plasma of estradiol-treated male murrel, Channa punctatus, by gel filtration chromatography. The native mass of VgC protein was 180 kDa, and it resolved as a single peptide of 100 kDa on SDS-PAGE. The peptide on subjecting to matrix-assisted laser desorption/ionization-time of flight produced a peptide mass fingerprint. On tandem mass spectrometry, some of these peptides showed mass to charge (m/z) ratio and amino acid sequence similarity with VgC peptides of other teleosts. Phylogenetic analysis revealed a similarity of murrel VgC with fish species of the order Perciformes. Semi-quantitative RT-PCR assay was developed to study expression of vgc gene at variable levels of estradiol exposure. Presence of VgC in males indicates that fish has been exposed to estrogens; hence, it can be used as a biomarker for estrogenic exposure.

Keywords

Incomplete vitellogenin (VgC) MALDI-TOF–MS/MS Channa punctatus RT-PCR 

Notes

Acknowledgments

This work was supported by Research Grant from the University of Delhi, Delhi. One of us (SP) is thankful to CSIR, New Delhi, for the award of a Senior Research Fellowship.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Department of ZoologyUniversity of DelhiDelhiIndia
  2. 2.Department of Zoology, Hindu CollegeUniversity of DelhiDelhiIndia

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