In vitro exposure to copper influences lipid metabolism in hepatocytes from grass carp (Ctenopharyngodon idellus)
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In the present study, three different copper (Cu) concentrations (control, 10 and 100 μM, respectively) and three incubation times (24, 48 and 96 h) were chosen to assess in vitro effect of Cu on lipid metabolism in hepatocytes of grass carp Ctenopharyngodon idellus. Increased glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and carnitine palmitoyltransferase I activities were observed in hepatocytes with increasing Cu concentration and exposure duration. Cu decreased mRNA levels of several lipogenic and lipolytic genes at 24 h. However, at 48 h, Cu down-regulated the process of lipogenesis but up-regulated that of lipolysis. The Cu-driven up-regulation of lipolytic genes was maintained after 96 h and accompanied by a decreased intracellular triglyceride accumulation, while no effect on lipogenic genes was shown. Thus, 96-h Cu exposure induced lipid depletion, possibly due to the up-regulation of lipolysis. Although in this process, lipogenesis might be up-regulated, it was not enough to compensate lipid consumption. Our study represents the first approach to concentration- and time-dependent in vitro effects of Cu on lipid metabolism of fish hepatocytes and provides new insights into Cu toxicity in fish at both enzymatic and molecular levels.
KeywordsHepatocytes Ctenopharyngodon idellus In vitro Lipid metabolism Cu
This work was funded by the Fundamental Research Funds for the Central Universities (Grant No. 2013PY073) and partly by National Natural Science Foundation of China (Grant No. 31001101).
Conflict of interest
The authors declare that there is no conflict of interest.
- Cowey CB, Walton MJ (1989) Intermediary metabolism. In: Halver JE (ed) Fish nutrition, 1st edn. Academic Press, New York, pp 259–329Google Scholar
- Eyckmans M, Benoot D, Van Raemdonck GA, Zegels G, Van Ostade XW, Witters E, Blust R, De Boeck G (2012) Comparative proteomics of copper exposure and toxicity in rainbow trout, common carp and gibel carp. Comp Biochem Physiol 7D:220–232Google Scholar
- He S, Liang XF, Qu CM, Huang W, Shen D, Zhang WB, Mai KS (2012) Identification, organ expression and ligand-dependent expression levels of peroxisome proliferator activated receptors in grass carp (Ctenopharyngodon idella). Comp Biochem Physiol 155C:381–388Google Scholar
- Heath AG (1987) Water pollution and fish physiology. CRC Press, Florida 245Google Scholar
- Hisar O, Sonmez AY, Beydemir S, Hisar SA, Yanik T, Cronin T (2009) Kinetic behaviour of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase in different tissues of rainbow Trout (Oncorhynchus mykiss) exposed to non-lethal concentrations of cadmium. Acta Vet Brno 78:179–185CrossRefGoogle Scholar
- Hu W, Zhi L, Zhuo MQ, Zhu QL, Zheng JL, Chen QL, Gong Y, Liu CX (2013) Purification and characterization of glucose 6-phosphate dehydrogenase (G6PD) from grass carp (Ctenopharyngodon idella) and inhibition effects of several metal ions on G6PD activity in vitro. Fish Physiol Biochem 39:637–647PubMedCrossRefGoogle Scholar