Fish Physiology and Biochemistry

, Volume 39, Issue 6, pp 1485–1496 | Cite as

Rearing temperature affects Senegalese sole (Solea senegalensis) larvae protein metabolic capacity

  • Catarina Campos
  • M. Filipa Castanheira
  • Sofia Engrola
  • Luísa M. P. Valente
  • Jorge M. O. Fernandes
  • Luís E. C. Conceição


The present work examined the short- and long-term effects of three rearing temperatures on protein metabolism and growth trajectories of Senegalese sole larvae using 14C-labelled Artemia protein as feed. A first feeding trial was performed on larvae reared at 15, 18 and 21 °C (at 26, 17 and 14 days post-hatching (dph), respectively) and a second trial conducted on post-larvae after transfer to the same rearing temperature (~20 °C) (49, 35 and 27 dph, in larvae initially reared at 15, 18 and 21 °C, respectively). Temperature greatly influenced larvae relative growth rate (RGR) and survival, since growth at 15 °C was severely depressed. Protein digestibility and retention was highest at 18 °C during the first trial (85.35 ± 1.16 and 86.34 ± 2.33 %, respectively). However, during the second trial, post-larvae from 15 °C had the highest feed intake and protein digestibility (3.58 ± 1.54 and 75.50 ± 1.35 %, respectively), although retention was similar between treatments. Furthermore, after transfer to 20 °C larvae from 15 °C experienced compensatory growth, which was observed until 121 dph, and confirmed by RGR values, which were significantly higher at 15 ºC than at 21 ºC or 18 ºC. Results from the present study show that Solea senegalensis larval development, survival and protein digestion and retention are highly affected by thermal history.


Rearing temperature Artemia intake Protein digestibility Retention efficiency Compensatory growth Solea senegalensis 



The authors would like to greatly acknowledge J. Sendão, H. Teixeira, F. Rocha and A. Santos from CCMAR (Portugal), for their invaluable help during the experimental set-up and sampling of Senegalese sole larvae. This work was funded by Project EPISOLE [PTDC/MAR/110547/2009] from Fundação para a Ciência e a Tecnologia (FCT), Portugal. C. Campos and S. Engrola acknowledge the financial support by Fundação para a Ciência e Tecnologia, Portugal, through grants SFRH/BD/43633/2008 and SFRH/BPD/49051/2008, respectively.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Catarina Campos
    • 1
    • 2
    • 3
  • M. Filipa Castanheira
    • 2
  • Sofia Engrola
    • 2
  • Luísa M. P. Valente
    • 1
  • Jorge M. O. Fernandes
    • 3
  • Luís E. C. Conceição
    • 2
    • 4
  1. 1.CIIMAR/CIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental and ICBAS–Instituto de Ciências Biomédicas de Abel SalazarPortoPortugal
  2. 2.CCMAR/CIMAR, Centro de Ciências do MarUniversidade do AlgarveFaroPortugal
  3. 3.Faculty of Biosciences and AquacultureUniversity of NordlandBodøNorway
  4. 4.SPAROS LdaCRIA, Universidade do AlgarveFaroPortugal

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