Fish Physiology and Biochemistry

, Volume 39, Issue 5, pp 1365–1370 | Cite as

In vitro evaluation of the effect of a high plant protein diet and nucleotide supplementation on intestinal integrity in meagre (Argyrosomus regius)

  • M. A. Sáenz de Rodrigáñez
  • J. Fuentes
  • F. J. Moyano
  • L. Ribeiro


In the present study, the protective effect of nucleotides over damages induced by the consumption of a diet containing a high amount of vegetable ingredients (560 g kg−1) in the intestinal epithelia of the meagre (Argyrosomus regius) was assessed by assays performed with an Ussing-type chamber. Two experimental feeds were prepared including or not a commercial mixture of nucleotides (1 g kg−1). Nucleotides significantly enhanced fish growth during the experiment. On the other hand, differences in the integrity and functionality of intestinal epithelia were evidenced by a change in the polarity of intestinal trans-epithelial potential. Samples of fish fed on the control diet showed a preferentially secretory short-circuit current, while those of fish receiving the nucleotide-supplemented diet showed a significantly lower and preferentially absorptive negative current. It is concluded that alterations of intestinal physiology juvenile meagre resulting from the intake of high amounts of plant ingredients could be minimized by nucleotide supplementation.


Feed fish Intestinal integrity Meagre Nucleotide Plant meal USSING chamber 



To BIOIBERICA and to SPAROS for providing, respectively, the nucleotides and the diets used in this research. To Fundación Alfonso Martín Escudero for supporting M. A. Saenz de Rodrigáñez, with a postdoctoral fellowship. This research used resources from project PTDC/MAR/104008/2008 (Ministry of Science and Higher Education, Portugal and European Social Funds), PROMAR projects (AQUACOR 31-03-05FEP-003 and NUTRICOR 31-03-05-FEP-19, co-financed by FEDER.) and FCT-“Ciência 2008 program”.


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • M. A. Sáenz de Rodrigáñez
    • 1
  • J. Fuentes
    • 2
  • F. J. Moyano
    • 3
  • L. Ribeiro
    • 1
  1. 1.Aquaculture Research StationIPMA - Portuguese Institute for the Sea and AtmosphereOlhãoPortugal
  2. 2.Center of Marine Sciences, CIMAR-LAUniversity of AlgarveFaroPortugal
  3. 3.Department of Applied BiologyUniversity of AlmeríaAlmeríaSpain

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