Homologue of Sox10 in Misgurnus anguillicaudatus: sequence, expression pattern during early embryogenesis
A number of genetic studies have established that Sox10 is a transcription factor associated with neurogenesis in vertebrates. We have isolated a homologue of Sox10 gene from the brain of Misgurnus anguillicaudatus by using homologous cloning and RACE method, designated as MaSox10b. The full-length cDNA of MaSox10b contained a 311 bp 5′UTR, a 312 bp 3′UTR and an ORF encoding a putative protein of 490 amino acids with a characteristic HMG-box DNA-binding domain of 79 amino acids (aa: 105–183). Phylogenetic tree shows that the MaSOX10b fits within the Sox10 clade and clusters firmly into Sox10b branches. During embryogenesis, MaSox10b was first detected in gastrulae stage. From somitogenesis stage and thereafter, distinct expression was observed in the medial neural tube, extending from the hindbrain through the posterior trunk. Taken together, these preliminary findings suggested that MaSox10b is highly conserved during vertebrate evolution and involved in a wide range of developmental processes including embryogenesis and neurogenesis.
KeywordsSox10b HMG-box Misgurnus anguillicaudatus Whole-mount in situ hybridization
This work is supported by grants from the National Natural Science Foundation of China (No. 31200923), Tianjin Key Laboratory of Animal and Plant Resistance Open Fund (No. 01046651012).
- Honoré SM, Aybar MJ, Mayor R (2003) Sox10 is required for the early development of the prospective neural crest in Xenopus embryos. Dev Biol 260:79–96Google Scholar
- Liu QY, Lu HJ, Zhang LH, Xie J, Shen WY, Zhang WM (2012) Homologues of sox8 and sox10 in the orange-spotted grouper Epinephelus coioides: sequences, expression patterns, and their effects on cyp19a1a promoter activities in vitro. Comp Biochem Physiol B Biochem Mol Biol 163:86–95PubMedCrossRefGoogle Scholar
- Pingault V, Bondurand N, Kuhlbrodt K, Goerich DE, Préhu MO, Puliti A, Herbarth B, Hermans-Borgmeyer I, Legius E, Matthijs G, Amiel J, Lyonnet S, Ceccherini I, Romeo G, Smith JC, Read AP, Wegner M, Goossens M (1998) Sox10 mutations in patients with Waardenburg-Hirschsprung disease. Nat Genet 18:171–173PubMedCrossRefGoogle Scholar
- Wilkinson DG (1992) In situ hybridization, a practical approach. Oxford University Press, OxfordGoogle Scholar