Fish Physiology and Biochemistry

, Volume 39, Issue 4, pp 733–747 | Cite as

Effect of azadirachtin on haematological and biochemical parameters of Argulus-infested goldfish Carassius auratus (Linn. 1758)

  • Saurav Kumar
  • R. P. Raman
  • Kundan Kumar
  • P. K. Pandey
  • Neeraj Kumar
  • B. Mallesh
  • Snatashree Mohanty
  • Abhay Kumar


Argulosis hampers aquaculture production and alters the host physiology and growth. Azadirachtin is recognized as a potential antiparasitic agent against Argulus sp. The present study aimed to investigate the effect of different concentration of azadirachtin solution on haematological and serum biochemical parameters of Argulus-infested goldfish Carassius auratus. Ninety Argulus-infested goldfish were randomly divided into six equal groups. Fish of group 1–5 were treated with azadirachtin solution through bath of 1, 5, 10, 15 and 20 mg L−1 as T1, T2, T3, T4 and T5, respectively, and group 6 was exposed to 2 % DMSO solution without azadirachtin and considered as negative control T0. Along with six treatment groups, a positive control T0+ of healthy goldfish free from Argulus infestation was also maintained. Parasitic mortality was evaluated after 3 days of consecutive bath treatment. After 7 days of post-treatment, the blood and serum were drawn from each of the treatment groups and haematological and serum biochemical parameters were evaluated. Total leucocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), blood glucose, total protein (TP), globulin, serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly (p < 0.05) high in negative control group when compared with positive control group. It could be concluded that Argulus infestation altered marked haematological and serum biochemical parameters. However, in treated groups complete elimination of Argulus was found in T4 and T5 groups. Also significant (p < 0.05) reduction in haematological and serum biochemical parameters of all the treatment groups were recorded in comparison with negative control group. In addition, T4 and T5 groups showed significantly (p < 0.05) high superoxide dismutase (SOD), catalase, total erythrocyte count (TEC) and haemoglobin (Hb). However, higher mean corpuscular haemoglobin concentration (MCHC), blood glucose and lactate dehydrogenase (LDH) levels in T5 group revealed that higher concentration of azadirachtin have notable effects on activity of vital tissues function and physiology of the host. Argulus spp. from infested goldfish could be eliminated using bath treatment with solution of azadirachtin having concentration of 15 mg L−1 and that also shifted haematological and serum biochemical parameters towards homeostasis.


Azadirachtin Argulus Haematology Biochemical Physiology Carassius auratus 



Authors are thankful to Dr. W.S. Lakra, Director and Vice chancellor, Central Institute of Fisheries Education, Mumbai, India for providing all the facilities required to carry out the study.

Supplementary material

10695_2012_9736_MOESM1_ESM.doc (734 kb)
Supplementary material 1 (DOC 1222 kb)


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Saurav Kumar
    • 1
  • R. P. Raman
    • 1
  • Kundan Kumar
    • 1
  • P. K. Pandey
    • 1
  • Neeraj Kumar
    • 1
  • B. Mallesh
    • 1
  • Snatashree Mohanty
    • 1
  • Abhay Kumar
    • 1
  1. 1.Central Institute of Fisheries EducationMumbaiIndia

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