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Intestinal absorption of amino acids in the Pacific bluefin tuna (Thunnus orientalis): in vitro lysine–arginine interaction using the everted intestine system

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Abstract

The interaction between lysine (Lys) and arginine (Arg) in the proximal intestinal region of Pacific bluefin tuna (Thunnus orientalis) was evaluated using the everted intestine method. This in vitro intestinal system has been shown to be an effective tool for studying the nutrient absorption without the need to handle the tuna fish in marine cages as needed for digestibility and amino acid (AA) absorption. We used a factorial design with two sets of variables: low and high Lys concentration (10 and 75 mM) and four different Arg concentrations (3, 10, 20, and 30 mM). Both amino acids were dissolved in marine Ringer solution with a basal amino acidic composition consisting of a tryptone solution (9 mg mL−1). No interaction was observed between the absorption of Lys and Arg during the first 10 min of the experiment when low concentration of Lys and Arg was used in the hydrolyzate solution. However, there seemed to be a positive effect on Lys absorption when both amino acids were at high concentrations (30 and 75 mM, respectively). This type of studies will led us to test different formulations and/or additives to better understand the efficiency of AA supplementation as an alternative to in situ studies that are difficult to follow to design with the Pacific Bluefin Tuna.

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Acknowledgments

This work was supported by project numbers SAGARPA/CONACyT 109150; CB-2009-01 and UABC 403/1/C/19/15. We thank Pacífico Aquaculture SA de CV for their kind donation of fish. Emmanuel Martínez-Montaño and Emyr Peña thank CONACyT for their graduate studies fellowships. We thank Nancy Boston for her English editorial work.

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  1. Emmanuel Martínez-Montaño

    Present address: Centro de Investigación y Desarrollo (CIEN Austral), CONICYT Regional R10C1002, Av. Los Pinos s/n, BalnearioPelluco, Puerto Montt, Chile

Authors and Affiliations

  1. Programa de Maestría y Doctorado en Oceanografía Costera, Facultad de Ciencias Marinas, Universidad Autónoma de Baja California (UABC), Ensenada, BC, Mexico

    Emmanuel Martínez-Montaño & Emyr Peña

  2. Instituto de Investigaciones Oceanológicas, Universidad Autónoma de Baja California, Km 107, carretera Tij-Eda, 22860, Ensenada, BC, Mexico

    María Teresa Viana

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  1. Emmanuel Martínez-Montaño
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  2. Emyr Peña
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  3. María Teresa Viana
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Correspondence to María Teresa Viana.

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Martínez-Montaño, E., Peña, E. & Viana, M.T. Intestinal absorption of amino acids in the Pacific bluefin tuna (Thunnus orientalis): in vitro lysine–arginine interaction using the everted intestine system. Fish Physiol Biochem 39, 325–334 (2013). https://doi.org/10.1007/s10695-012-9702-5

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