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Fish Physiology and Biochemistry

, Volume 38, Issue 5, pp 1419–1426 | Cite as

Dietary tryptophan changes serum stress markers, enzyme activity, and ions concentration of wild common carp Cyprinus carpio exposed to ambient copper

  • Seyyed Morteza Hoseini
  • Seyed Abbas Hosseini
  • Mohammad Soudagar
Article

Abstract

Effect of dietary tryptophan (TRP) on copper toxicity was investigated in common carp, Cyprinus carpio. Fish were randomly distributed into two triplicate treatments (control and TRP) and fed control (TRP = 3.1 g kg−1) or TRP (TRP = 8.1 g kg−1) diets over a 2-week period. Then, both treatments were subjected to 10 mg l−1 copper sulfate over a 7-day period. Mortality and serum cortisol, glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), sodium, potassium, and chloride levels were recorded at 0, 24, 72, 120, and 168 h post-copper exposure. There was no mortality in the TRP group, whereas the control group mortality was started at 120 h post-copper exposure (21.7 ± 7.6 %) and reached 61.7 ± 10.4 % at the end of the experiment. Cortisol, glucose, ALT, and AST levels significantly (P < 0.05) increased after copper exposure in both groups; however, the control group showed significantly higher levels than the TRP group. In both groups, sodium decreased after copper exposure and did not return to the pre-exposure values. Potassium levels significantly increased 24 h post-copper exposure in both groups and remained significantly elevated in the control group at 72 h, when fell to the pre-exposure values in the TRP group. No significant changes were observed in the chloride levels in any groups. It is concluded that dietary TRP is capable to increase common carp tolerance to copper toxicity via mitigating copper-induced stress rather than restoring gill dysfunction and hydromineral imbalance.

Keywords

Common carp Copper Tryptophan Toxicity Stress 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Seyyed Morteza Hoseini
    • 1
  • Seyed Abbas Hosseini
    • 1
  • Mohammad Soudagar
    • 1
  1. 1.Department of Fisheries, Faculty of Fisheries and EnvironmentGorgan University of Agricultural Sciences and Natural ResourcesGorganIran

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