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Fish Physiology and Biochemistry

, Volume 38, Issue 2, pp 499–509 | Cite as

Acute and chronic effects of endosulfan on the haemato-immunological and histopathological responses of a threatened freshwater fish, spotted murrel, Channa punctatus

  • Kamal Sarma
  • A. K. Pal
  • N. P. Sahu
  • R. S. Dalvi
  • Nirupama Chatterjee
  • S. C. Mukherjee
  • Kartik Baruah
Article

Abstract

Two experiments, one short-term and one long-term, were conducted to elucidate the acute and chronic effects, respectively, of endosulfan exposure on the haemato-immunological and histopathological responses of Channa puncatatus. In the short-term study, fish were exposed to sublethal endosulfan (8.1 μg l−1) for 12, 24, 36, 48, 72 and 96 h. In the long-term study, fish were fed with normal diet and simultaneously either exposed to endosulfan (1.2 μg l−1) for 90 days or not. Results showed that the ascorbic acid levels in both the liver and the muscle decreased significantly (P < 0.05) by acute and chronic endosulfan exposure. The haemoglobin (Hb) level reduced significantly (P < 0.05) by 15.5% within 12 h of acute endosulfan exposure, further decreased by 25.8% after 24 h of exposure, however, thereafter the values increased and at the end of 72 h returned to normal levels. Almost similar trend was observed for the erythrocyte (RBC) count. The WBC count and the nitroblue tetrazolium (NBT) value showed a general increasing trend with increase in the duration of acute endosulfan exposure. The chronic exposure of C. punctatus to endosulfan significantly (P < 0.05) lowered the Hb level, RBC and WBC counts, NBT reduction value and the plasma parameters such as plasma protein, albumin (A) and globulin (G) compared with that of the control (except for A/G ratio). Endosulfan exposure also severely altered the liver histological structure. Overall results indicated that both short-term acute and long-term chronic endosulfan exposure had a significant impact on the haemato-immunological parameters and tissue histopathology of C. punctatus.

Keywords

Endosulfan Channa punctatus Haematology Histology Immune responses 

Notes

Acknowledgments

The authors are grateful to the Director, Central Institute of Fisheries Education, Mumbai, for providing all the facilities for this study. The first author acknowledges the financial support from Indian Council of Agricultural Research, New Delhi.

References

  1. Auffret M, Rousseau S, Boutet I, Tanguy A, Baron J, Moraga D, Duchemin M (2006) A multiparametric approach for monitoring immunotoxic responses in mussels from contaminated sites in Western Mediterranea. Ecotoxicol Environ Saf 63:393–405PubMedCrossRefGoogle Scholar
  2. Bernabó I, Brunelli E, Berg C, Bonacci A, Tripepi S (2008) Endosulfan acute toxicity in Bufo bufo gills: ultrastructural changes and nitric oxide synthase localization. Aquat Toxicol 86:447–456PubMedCrossRefGoogle Scholar
  3. Betoulle S, Duchiron C, Deschaux P (2000) Lindane increases in vitro respiratory burst activity and intracellular calcium levels in rainbow trout (Oncorhynchus mykiss) head kidney phagocytes. Aquat Toxicol 48:211–221PubMedCrossRefGoogle Scholar
  4. CAMP (1998) Report of the workshop on conservation assessment and management plan (CAMP) for freshwater fishes of India. Zoo Outreach Organization and NBFGR, Lucknow, India, 22–26 September 1997, pp 1–156Google Scholar
  5. Chuah TS, Loh JY, Hii YS (2007) Acute and chronic effects of the insecticide-endosulfan on freshwater cladoceran, Moina macrocopa Straus. Bull Environ Contam Toxicol 79:557–561PubMedCrossRefGoogle Scholar
  6. Das BK, Mukherjee SC (2000) A histological study of carp (Labeo rohita) exposed to hexachlorocyclohexane. Vet Arhiv 70:169–180Google Scholar
  7. Doumas BT, Watson W, Biggs HG (1971) Albumin standards and measurement of serum albumin with bromocresol green. Clinica Chimica Acta 31:87–96CrossRefGoogle Scholar
  8. EFSA (European Food Safety Authority) (2005) Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to endosulfan as undesirable substance in animal feed. EFSA J 234:1–29Google Scholar
  9. EJF (2002) End of the road for endosulfan: a call for action against a dangerous pesticide. Environmental Justice Foundation, LondonGoogle Scholar
  10. Esteban MA, Mulero M, Cuesta A, Ortuno J, Meseguer J (2000) Effects of injecting chitin particles to the innate immune response of gilthead seabream (Sparus aurata L.). Fish Shellfish Immunol 10:543–554PubMedCrossRefGoogle Scholar
  11. Girón-Pérez MI, Montes-López M, García-Ramírez LA, Romero-Bañuelos CA, Robledo-Marenco ML (2008) Effect of sublethal concentrations of endosulfan on phagocytic and hematological parameters in Nile Tilapia (Oreochromis niloticus). Bull Environ Contam Toxicol 80:266–269PubMedCrossRefGoogle Scholar
  12. Glover CN, Petri D, Tollefsen KE, Jørum N, Handy RD, Berntssen MHG (2007) Assessing the sensitivity of Atlantic salmon (Salmo salar) to dietary endosulfan exposure using tissue biochemistry and histology. Aquat Toxicol 84:346–355PubMedCrossRefGoogle Scholar
  13. Hamoutene D, Payne JF, Volkoff H (2008) Effects of tebufenozide on some aspects of lake trout (Salvelinus namaycush) immune response. Ecotoxicol Environ Saf 69:173–179PubMedCrossRefGoogle Scholar
  14. Heath AG (1995) Water pollution and fish physiology, 2nd edn. Lewis publishers, LondonGoogle Scholar
  15. Hota AK, Mishra DK, Tripathy PC (1991) Metabolic effects of kilex carbaryl on a freshwater teleost, Channa punctatus (Bloch). In: Agrawal VP, Abidi SAH, Verma GP (eds) Environmental impact on aquatic and terrestrial habitats, December 1991. Berhampur Society of Biosciences, Muzaffarnagar, pp 335–342Google Scholar
  16. James R, Sampath K (1996) Individual and combined effects of carbaryl and methylparathion on leucocytes and their recovery in Heteropneustes fossilis. In: Mishra SR (ed) Assessment of water pollution. ARH publishing cooperation, New Delhi, pp 417–421Google Scholar
  17. Jayaram KC (1981) The freshwater fishes of India, Pakistan, Bangladesh, Burma and Sri Lanka: a handbook. Zoological Survey of India, CalcuttaGoogle Scholar
  18. Jenkins F, Smith J, Rajanna B, Shameem U, Umadevi K, Sanhya V, Madhavi R (2003) Effect of sublethal concentration of endosulfan on hematological and serum biochemical parameters in the carp, Cyprnus carpio. Bull Environ Contam Toxicol 70:993–997PubMedCrossRefGoogle Scholar
  19. Kole RK, Banerjee H, Bhattacharyya A (2001) Monitoring of market fish samples for Endosulfan and Hexachlorocyclohexane residues in and around Calcutta. Bull Environ Contam Toxicol 67:554–559PubMedCrossRefGoogle Scholar
  20. Lambeth JD (1988) Activation of the respiratory burst oxidase in neutrophils: on the role of membrane-derived second messengers, Ca2+ and protein kinase C. J Bioenerg Biomembr 20:709–733PubMedCrossRefGoogle Scholar
  21. Luskova V (1996) Annual cycles and normal values of hematological parameters in fishes. Acta Sci Nat Brno 31:1–70Google Scholar
  22. Mauck WL, Mehrle PM, Mayer FL (1978) Effect of polychlorinated biphenyl Arochor@ 1254 on growth, survival and bone development in brook trout, Salvelinus fontinalis. J Fish Res Board Can 35:1084–1088CrossRefGoogle Scholar
  23. McLeay DJ, Brown DA (1974) Sensitivity of blood cell counts in juvenile coho salmon Oncorhynchus kisutch to stressors including sublethal concentrations of pulp mill effluent and zinc. J Fish Res Biocon 31:1043–1049CrossRefGoogle Scholar
  24. Metelev VV, Kanaev AL, Diasokhva NG (1971) Water toxicity. Amerind Publishing Cooperation Private Limited, New DelhiGoogle Scholar
  25. Mikula P, Modra H, Nemethova D, Groch L, Svobodova Z (2008) Effects of subchronic exposure to LASSO MTX® (Alachlor 42% W/V) on hematological indices and histology of the common carp, Cyprinus carpio L. Bull Environ Contam Toxicol 81:475–479PubMedCrossRefGoogle Scholar
  26. Mulcahy MF (1971) Serum protein changes associated with ulcerative dermal necrosis (UDN) in the trout Salmo trutta L. J Fish Biol 3:199–201CrossRefGoogle Scholar
  27. Naqvi SM, Vaishnavi C (1993) Bioaccumulative potential and toxicity of endosulfan insecticide to non-target animals. Comp Biochem Physiol 105C:347–361Google Scholar
  28. Narayan AS, Singh BBN (1991) Histopathological lesions in Heteropneutus fossilis subject to acute thiodon toxicity. Acta Hydrochem Hydrobiol 19:235–243CrossRefGoogle Scholar
  29. Petri D, Glover CN, Ylving S, Kolås K, Fremmersvik G, Waagbø R, Berntssen MHG (2006) Sensitivity of Atlantic salmon (Salmo salar) to dietary endosulfan as assessed by haematology, blood biochemistry, and growth parameters. Aquat Toxicol 80:207–216PubMedCrossRefGoogle Scholar
  30. Reinhold JG (1953) Manual determination of serum total protein, albumin and globulin fractions by Biuret method. In: Reiner M (ed) Standard method of clinical chemistry. Academic Press, New York, p 88Google Scholar
  31. Roberts RJ (1989) Nutritional pathology of teleosts. In: Roberts RJ (ed) Fish pathology. Bailliere Tindall, London, pp 337–362Google Scholar
  32. Roe JH, Keuther CA (1943) The determinations of ascorbic acid in whole blood and urine through the 2, 4-dinitrophenylhydrazine (DNPH) derivative of dehydroascorbic acid. J Biol Chem 147:399–407Google Scholar
  33. Sarma K, Pal AK, Mukherjee SC, Datta S (2003) Acute toxicity of endosulfan of freshwater teleost, Channa punctatus (Bloch). J Enviorn Res 13:80–84Google Scholar
  34. Sarma K, Pal AK, Sahu NP, Ayyappan S, Baruah K (2010a) Dietary high protein, vitamin C mitigates endosulfan toxicity in the spotted murrel, Channa punctatus (Bloch, 1793). Sci Total Environ 408:2179CrossRefGoogle Scholar
  35. Sarma K, Pal AK, Sahu NP, Mukherjee SC, Baruah K (2010b) Biochemical and histological changes in the brain tissue of spotted murrel, Channa punctatus (Bloch), exposed to endosulfan. Fish Physiol Biochem 36:597–603PubMedCrossRefGoogle Scholar
  36. Sastry KV, Sharma KS (1979) Endrin induced hepatic injury in Channa punctatus (Ham). Ind J Fish 26:250–253Google Scholar
  37. Secombes CJ (1990) Isolation of salmonid macrophage and analysis of their killing ability. In: Stolen JS, Fletcher TC, Anderson DP, Roberson BS, Van WB, Winkel M (eds) Techniques in fish immunology. SOS Publication, New Jersey, pp 137–152Google Scholar
  38. Sen G, Kumar BM, Patel PN (1992) Toxic effects of zinc on liver and brain of the fish, Channa punctatus (Bloch). Environ Ecol 10:742–744Google Scholar
  39. Sindermann CJ (1983) An examination of some relationships between pollution and disease. Rapp PV Reun Cons Int Explor Mer 182:37–43Google Scholar
  40. Stasiack AS, Bauman CP (1996) Neutrophil activity as a potent indicator for concomitant analysis. Fish Shellfish Immunol 6:537–539CrossRefGoogle Scholar
  41. Sunderam RIM, Thompson GB, Chapman JC, Cheng DMH (1994) Acute and chronic toxicity of endosulfan to two Australian cladocerans and their applicability in deriving water quality criteria. Arch Environ Contam Toxicol 27:541–545CrossRefGoogle Scholar
  42. Svobodova Z, Luskova V, Drastichova J, Svoboda M, Zlabek V (2003) Effect of deltamethrin on haematological indices of common carp (Cyprinus carpio L.). Acta Vet Brno 72:79–85CrossRefGoogle Scholar
  43. Thomas P, Bally M, Neff JM (1982) Ascorbic acid status of mullet, Mugil cephalus Linn., exposed to cadmium. J Fish Biol 20:183–196CrossRefGoogle Scholar
  44. Wedemeyer GA, Gould RW, Yasutake WT (1983) Some potentials and limits of the leukocrit test as a fish health assessment method. J Fish Biol 23:711–716CrossRefGoogle Scholar
  45. Wiegertjes GF, Stet RJM, Parmentier HK, Muiswinkle WB (1996) Immunogenetics and disease resistance in fish: a comparative approach. Develop Comp Immunol 20:365–381CrossRefGoogle Scholar
  46. Winston G, Diguila R (1991) Pro-oxidant and antioxidant mechanisms in aquatic organisms. Aquat Toxicol 19:137–161CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Kamal Sarma
    • 1
  • A. K. Pal
    • 2
  • N. P. Sahu
    • 2
  • R. S. Dalvi
    • 2
  • Nirupama Chatterjee
    • 2
  • S. C. Mukherjee
    • 3
  • Kartik Baruah
    • 4
  1. 1.Division of Fisheries ScienceCentral Agricultural Research Institute, ICARPort BlairIndia
  2. 2.Division of Fish Nutrition and BiochemistryCentral Institute of Fisheries EducationVersova, MumbaiIndia
  3. 3.Division of Fish Pathology and MicrobiologyCentral Institute of Fisheries EducationVersova, MumbaiIndia
  4. 4.Laboratory of Aquaculture and Artemia Reference Centre, Department of Animal ProductionGhent UniversityGhentBelgium

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