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Fish Physiology and Biochemistry

, Volume 37, Issue 4, pp 725–732 | Cite as

Diet supplemented with probiotic for Nile tilapia in polyculture system with marine shrimp

  • Adolfo Jatobá
  • Felipe do Nascimento Vieira
  • Celso Carlos Buglione-Neto
  • José Luiz Pedreira Mouriño’
  • Bruno  Corrêa Silva
  • Walter Quadros Seiftter
  • Edemar Roberto Andreatta
Article

Abstract

The aim of this study was to assess the effect of a probiotic (Lactobacillus plantarum) supplemented diet on Nile tilapia (Oreochromis niloticus) in a polyculture system with marine shrimp (Litopenaeus vannamei) as regards culture performance, hematology, and gut bacterial microbiota. Ten 20-m² pens were arranged in one earthen pond and stocked with 2 fish (41.9 g) m−2 and 10 shrimp (2.3 g) m−2, in total of 40 Nile tilapias and 200 shrimp per experimental unit. Tilapia groups in five of the experimental units were fed a commercial diet supplemented with L. plantarum and the other five with an unsupplemented commercial diet (control). After 12 weeks of culture, the tilapia groups fed the probiotic-supplemented diet presented values 13.6, 7.5, and 7.1% higher for feed efficiency, yield, and final weight, respectively. Viable culturable heterotrophic bacteria counts were reduced, and the number of lactic acid bacteria was increased in the gut of fish and shrimp fed the probiotic-supplemented diet. Hematological analyses showed higher number of thrombocytes and leukocytes in tilapia fed the supplemented diet. L. plantarum utilized in this study colonized the gut of tilapia and shrimp and resulted in reduced number of total bacteria and increased tilapia final weight and feed efficiency.

Keywords

Oreochromisniloticus Litopenaeusvannamei Hematology Bacterial microbiota Growth 

Notes

Acknowledgments

The authors are grateful to Professor Elpídio Beltrame (in memoriam) for his dedication during the preparation of this study; to Guabi for providing the feed; to Aqua Sul hatchery for providing the Nile tilapia juveniles; to CNPq for the scholarship; and to Secretaria Especial de Aquicultura e Pesca for the financial support.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Adolfo Jatobá
    • 1
    • 2
  • Felipe do Nascimento Vieira
    • 1
  • Celso Carlos Buglione-Neto
    • 1
  • José Luiz Pedreira Mouriño’
    • 1
  • Bruno  Corrêa Silva
    • 1
  • Walter Quadros Seiftter
    • 1
  • Edemar Roberto Andreatta
    • 1
  1. 1.Laboratório de Camarões Marinhos, Departamento de AquiculturaUniversidade Federal de Santa Catarina (UFSC)FlorianópolisBrazil
  2. 2.Instituto Federal CatarinenseAraquariBrazil

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