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Fish Physiology and Biochemistry

, Volume 36, Issue 3, pp 749–755 | Cite as

Effect of dietary ascorbate on lipogenesis and lipolysis activities in black sea bream, Acanthopagrus schlegelii

  • H. Ji
  • A. D. Om
  • T. Yoshimatsu
  • T. Umino
  • H. Nakagawa
  • S. Sakamoto
Article

Abstract

To assess the effect of dietary ascorbate on lipid metabolism, 1-year black sea bream (Acanthopagrus schlegelii) were reared on a casein-based purified diet and an ascorbate fortified diet (1,100 mg of l-ascorbyl-2- monophosphate-Mg/kg diet). The fortified ascorbate was effectively incorporated into the fish body and elevated muscle carnitine content. Fortifications of dietary ascorbate depressed activities of glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as lipogenic enzymes in the hepatopancreas and intraperitoneal fat body. Starvation after feeding experiment activated carnitine palmitoyltransferase as a lipolysis enzyme in the hepatopancreas in both control and vitamin C(VC) groups, while the lipolysis activity was significantly higher in VC group. These results confirmed that dietary ascorbate depressed lipogenesis and activated lipolysis, i.e., influenced the lipid metabolism of black sea bream.

Keywords

Ascorbate Carnitine Lipid metabolism Lipogenesis Lipolysis Acanthopagrus schlegelii 

Notes

Acknowledgments

We thank the staff and students of the Graduate school of Bioresource and Bioenbironmental Science, Kyushu University for their cooperation in the study.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • H. Ji
    • 1
    • 2
  • A. D. Om
    • 2
    • 3
  • T. Yoshimatsu
    • 4
  • T. Umino
    • 2
  • H. Nakagawa
    • 2
  • S. Sakamoto
    • 5
  1. 1.College of Animal Science and TechnologyNorthwest A&F UniversityShaanxiChina
  2. 2.Graduate School of Biosphere ScienceHiroshima UniversityHigashi-HiroshimaJapan
  3. 3.Marine Finfish Production and Research CenterTerengganuMalaysia
  4. 4.Graduate School of BioresourcesMie UniversityMieJapan
  5. 5.Oriental Yeast Co. Ltd.ChibaJapan

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