Effect of dietary ascorbate on lipogenesis and lipolysis activities in black sea bream, Acanthopagrus schlegelii
- 184 Downloads
To assess the effect of dietary ascorbate on lipid metabolism, 1-year black sea bream (Acanthopagrus schlegelii) were reared on a casein-based purified diet and an ascorbate fortified diet (1,100 mg of l-ascorbyl-2- monophosphate-Mg/kg diet). The fortified ascorbate was effectively incorporated into the fish body and elevated muscle carnitine content. Fortifications of dietary ascorbate depressed activities of glucose-6-phosphate dehydrogenase and NADP-isocitrate dehydrogenase as lipogenic enzymes in the hepatopancreas and intraperitoneal fat body. Starvation after feeding experiment activated carnitine palmitoyltransferase as a lipolysis enzyme in the hepatopancreas in both control and vitamin C(VC) groups, while the lipolysis activity was significantly higher in VC group. These results confirmed that dietary ascorbate depressed lipogenesis and activated lipolysis, i.e., influenced the lipid metabolism of black sea bream.
KeywordsAscorbate Carnitine Lipid metabolism Lipogenesis Lipolysis Acanthopagrus schlegelii
We thank the staff and students of the Graduate school of Bioresource and Bioenbironmental Science, Kyushu University for their cooperation in the study.
- Akand AM, Sato M, Yoshinaka R, Ikeda S (1993) Effect of dietary ascorbic acid on lipid and fatty acid composition in rainbow trout Salmo gairnrei. In: The sixth international symposium on fish nutrition and feeding, Hobart, 4–7 October 1993Google Scholar
- Ginter E, Bobek P (1981) The influence of vitamin C on lipid metabolism. In: Counsell JN, Hornig DH (eds) Vitamin C/ascorbic acid. Applied Science Publication, London, pp 299–347Google Scholar
- Halver JE (1989) Vitamin C requirements for fish growth and health. In: Shiau SY (ed) Progress in fish nutrition. Marine Food Science, Keelung, pp 27–39Google Scholar
- Ji H, Om AD, Umino T, Nakagawa H (2003a) Comparison of ascorbate content in hatchery-reared and wild black sea bream, Acanthopagrus schlegeli (Pisces). Proc 20th Symp Trace Nutr Res 2003:61–65Google Scholar
- Mustafa MG, Umino T, Nakagawa H (1997) Limited synergistic effect of dietary Spirulina on vitamin C nutrition of red sea bream Pagrus major. J Marine Biotechnol 5:129–132Google Scholar
- Takeda Y, Hara M (1954) Significance of ferrous ion and ascorbate acid in the operation of the tricarboxylic acid cycle. J Biol Chem 214:657–670Google Scholar