Fish Physiology and Biochemistry

, Volume 36, Issue 3, pp 677–686 | Cite as

Effects of fish CYP inducers on difloxacin N-demethylation in kidney cell of Chinese idle (Ctenopharyngodon idellus)

  • Ling Zhi Yu
  • Xian Le Yang
  • Xiang Ling Wang
  • Wen Juan Yu
  • Kun Hu


A drug–drug interaction occurs when the effect of one drug is altered by the presence of another drug which is generally associated with the induction of cytochrome P450s (CYPs) activity. Thus, unexpected treatment failures often happen resulting from inappropriate coadministration in fisheries. However, little information is available about CYP induction in fish. The reaction of difloxacin (DIF) biotransformation to sarafloxacin (SAR) belongs to N-demethylation catalyzed mainly by CYP(s). In order to supply useful information on CYP induction, the present study assessed the effects of fish-specific CYP inducers on DIF N-demethylation and enzyme kinetics in kidney cell of Chinese idle (CIK; grass carp (Ctenopharyngodon idellus)) by RP-HPLC. Results demonstrated that the amounts of SAR formation and enzymatic parameters Clint and Vmax were significantly increased due to β-naphthoflavone (BNF) pretreatment. Therefore, we suggest that CYP1A may be involved in DIF N-demethylation in CIK. This study provides instructive information to ensure treatment success via avoiding CYP induction in fisheries.


Chinese idle CYP Difloxacin Grass carp Kinetics RP-HPLC Sarafloxacin 





Kidney cell of Chinese idle


Cytochrome P450s




Ethyl alcohol


Grass carp liver cell line




Reversed-phase high-performance liquid chromatography





This work was financially supported by the National Natural Science Foundation of China (No. 30371109).


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Ling Zhi Yu
    • 1
  • Xian Le Yang
    • 1
  • Xiang Ling Wang
    • 2
  • Wen Juan Yu
    • 1
  • Kun Hu
    • 1
  1. 1.Aquatic Pathogen Collection Centre of Ministry of AgricultureShanghai Ocean UniversityShanghaiChina
  2. 2.Shanghai Medicilon & MPI Inc.ShanghaiChina

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