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Fish Physiology and Biochemistry

, Volume 36, Issue 3, pp 523–529 | Cite as

DNA content of hepatocyte and erythrocyte nuclei of the spined loach (Cobitis taenia L.) and its polyploid forms

  • Dorota Juchno
  • Bozena Lackowska
  • Alicja Boron
  • Wincenty Kilarski
Article

Abstract

We analyzed the DNA content of hepatocyte and erythrocyte nuclei of the spined loach Cobitis taenia (diploid) and its allopolyploid forms. Twenty triploid females and one tetraploid were used. At least 20,000 hepatocyte and erythrocyte nuclei were acquired and analyzed by flow cytometry. C. taenia erythrocyte nuclei contain 3.15 ± 0.21 pg of DNA and the hepatocyte nuclei 4.45 ± 0.46 pg of DNA. Triploid Cobitis have 5.08 ± 0.41 pg of DNA in erythrocyte nuclei and 6.11 ± 0.40 pg of DNA in hepatocyte nuclei, whereas the tetraploid erythrocyte and hepatocyte nuclei contained 6.60 and 7.40 pg of DNA, respectively. In general, the DNA contents correlate positively with the ploidy level of the fish investigated. The DNA content variation in the hepatocyte and erythrocyte nuclei may be due to differences in extent of chromatin condensation, which is more pronounced in the erythrocyte than hepatocyte nuclei, or to the several orders of ploidy that occur in the parenchymal liver cells.

Keywords

Cobitis DNA Polyploidy Spined loach 

Notes

Acknowledgments

We thank Professor Elżbieta Pyza chief of the Department for providing facility used during this study and hospitality for one of us (W·K.), Dr. Gregory Tylko who introduced me (W·K.) to the techniques of Flow Cytometry. We would like to thank N.J. Severs for critical comments on the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Dorota Juchno
    • 1
  • Bozena Lackowska
    • 2
  • Alicja Boron
    • 1
  • Wincenty Kilarski
    • 3
  1. 1.Department of ZoologyUniversity of Warmia and MazuryOlsztynPoland
  2. 2.Department of Cancer PathologyOncology CenterKrakowPoland
  3. 3.Department of Cytology and Histology, Institute of ZoologyJagiellonian UniversityKrakowPoland

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