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Modulation of key enzymes of glycolysis, gluconeogenesis, amino acid catabolism, and TCA cycle of the tropical freshwater fish Labeo rohita fed gelatinized and non-gelatinized starch diet

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Abstract

A 60-day experiment was conducted to study the effect of dietary gelatinized (G) and non-gelatinized (NG) starch on the key metabolic enzymes of glycolysis (hexokinase, glucokinase, pyruvate kinase, and lactate dehydrogenase), gluconeogenesis (glucose-6 phosphatase and fructose-1,6 bisphosphatase), protein metabolism (aspartate amino transferase and alanine amino transferase), and TCA cycle (malate dehydrogenase) in Labeo rohita juveniles. In the analysis, 234 juveniles (2.53 ± 0.04 g) were randomly distributed into six treatment groups each with three replicates. Six semi-purified diets containing NG and G cornstarch, each at six levels of inclusion (0, 20, 40, 60, 80, and 100) were prepared viz., T1 (100% NG, 0% G starch), T2 (80% NG, 20% G starch), T3 (60% NG, 40% G starch), T4 (40% NG, 60% G starch), T5 (20% NG, 80% G starch), and T6 (0% NG, 100% G starch). Dietary G:NG starch ratio had a significant (P < 0.05) effect on the glycolytic enzymes, the highest activities were observed in the T6 group and lowest in the T1 group. On the contrary, the gluconeogenic enzymes, the glucose-6-phosphatase and fructose-1,6 bisphosphatase activities in the organs, liver and kidney were recorded highest in the T1 group and lowest in the T6 group. The liver aspartate amino transferase activity showed an increasing trend with the decrease in the dietary G level. However, the muscle aspartate amino transferase activity was not significantly (P > 0.05) influenced by the type of dietary starch. The alanine amino transferase activity in both liver and muscle showed an increasing trend with the decrease in the dietary G level. The liver and muscle malate dehydrogenase activities were lowest in the T6 group and highest in the T1 group. Results suggest that NG (100%) starch diet significantly induced more the enzyme activities of amino acid metabolism, gluconeogenesis, and TCA cycle, whereas partial or total replacement of raw starch by gelatinized starch increased the glycolytic enzyme activity.

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References

  • Alexis MN, Papaparaskeva-Papoutsoglou E (1986) Aminotransferase activity in the liver and white muscle of Mugil capito fed diets containing different levels of protein and carbohydrate. Comp Biochem Physiol 83B:245–249

    CAS  Google Scholar 

  • Bergot F (1993) Digestibility of native starch of various botanical origins by rainbow trout (Oncorhynchus mykiss). In: Kaushik SJ, Luquet P (eds) Fish nutrition in practice. Proceedings of the IV international symposium on nutrition and feeding, Les Colloques, INRA, no. 61, pp 857–865

  • Bergot F, Bréque J (1983) Digestibility of starch by rainbow trout: effects of the physical state of starch and of the intake level. Aquaculture 22:81–96. doi:10.1016/0044-8486(81)90135-6

    Article  Google Scholar 

  • Catacutan MR, Coloso RM (1998) Growth of juvenile Asian sea bass, Lates calcarifer, fed varying carbohydrate and lipid levels. Aquaculture 149:137–144. doi:10.1016/S0044-8486(96)01432-9

    Article  Google Scholar 

  • Cho CY, Kaushik SJ (1990) Nutritional energetics in fish: energy and protein utilization in rainbow trout (Salmo gairdneri). In: Bourne GH (ed) Aspects of food production, consumption and energy values. World Rev Nutr Diet, Karger, Basel, 61:132–172

  • Couto A, Enes P, Peres H, Oliva-Teles A (2008) Effect of water temperature and dietary starch on growth and metabolic utilization of diets in gilthead sea bream (Sparus aurata) juveniles. Comp Biochem Physiol A 151:45–50. doi:10.1016/j.cbpa.2008.05.013

    Article  CAS  Google Scholar 

  • Enes P, Panserat S, Kaushik S, Oliva-Teles A (2006) Effect of normal and waxy maize starch on growth, food utilization and hepatic glucose metabolism in European sea bass (Dicentrarchus labrax) juveniles. Comp Biochem Physiol 143A:89–96

    Article  CAS  Google Scholar 

  • Enes P, Panserat S, Kaushik S, Oliva-Teles A (2008) Hepatic glucokinase and glucose-6-phosphatase responses to dietary glucose and starch in Gilthead Sea bream (Sparus aurata) juveniles reared at two temperatures. Comp Biochem Physiol A 149:80–86. doi:10.1016/j.cbpa.2007.10.012

    Article  CAS  Google Scholar 

  • FAO (2001) FAO yearbook on fishery statistics. Rome, Italy

    Google Scholar 

  • Fernandez F, Miquel AG, Cordoba M, Varas M, Metón I, Caseras A, Baanante IV (2007) Effects of diets with distinct protein-to-carbohydrate ratios on nutrient digestibility, growth performance, body composition and liver intermediary enzyme activities in gilthead sea bream (Sparus aurata, L.) fingerlings. J Exp Mar Biol Ecol 343:1–10. doi:10.1016/j.jembe.2006.10.057

    Article  CAS  Google Scholar 

  • Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400

    CAS  Google Scholar 

  • Freeland RA, Harper AL (1959) The study of metabolic pathway by means of adaptation. J Biol Chem 234:1350–1354

    Google Scholar 

  • Furuichi M, Yone Y (1982) Change in activities of hepatic enzymes related to carbohydrate metabolism of fishes in glucose and insulin-glucose tolerance tests. Bull Jpn Soc Sci Fish 48:463–466

    Article  CAS  Google Scholar 

  • Fynn-Aikins K, Hung SSO, Hughes SG (1993) Effects of feeding a high level of d-glucose on liver function in juvenile white sturgeon (Acipenser transmontanus). Fish Physiol Biochem 12:317–325. doi:10.1007/BF00004416

    Article  CAS  Google Scholar 

  • Gaye-Siessegger J, Focken U, Becker K (2006) Effect of dietary protein/carbohydrate ratio on activities of hepatic enzymes involved in the amino acid metabolism of Nile tilapia, Oreochromis niloticus (L.). Fish Physiol Biochem 32:275–282. doi:10.1007/s10695-006-9000-1

    Article  CAS  Google Scholar 

  • Guraya HS, Toledo RT (1993) Determining gelatinized starch in a dry starchy product. J Food Sci 58:888. doi:10.1111/j.1365-2621.1993.tb09384.x

    Article  CAS  Google Scholar 

  • Hilton JW, Slinger SJ (1983) Effect of wheat bran replacement of wheat midlings in extrusion processed (floating) diets on the growth of juvenile rainbow trout (Salmo gairdneri). Aquaculture 5:201–210. doi:10.1016/0044-8486(83)90091-1

    Article  Google Scholar 

  • Hung SSO, Fynn-Aikins FK, Lutes PB, Xu R (1989) Ability of juvenile white sturgeon (Acipenser transmontanus) to utilize different carbohydrate sources. J Nutr 119:727–733

    Article  CAS  Google Scholar 

  • Hutchins CG, Rawles SD, Gatlin DMIII (1998) Effects of dietary carbohydrate kind and level on growth, body composition and glycemic response of juvenile sunshine bass (Morone chrysops female × M. saxatilis male). Aquaculture 161:187–199. doi:10.1016/S0044-8486(97)00269-X

    Article  CAS  Google Scholar 

  • Jeong KS, Takeuchi T, Okamoto N, Watanabe T (1992a) The effects of dietary gelatinized ratios at different dietary energy on growth and characteristic of blood in rainbow trout fingerlings. Nippon Siusan Gakkaishi 58:937–944

    Article  Google Scholar 

  • Jeong KS, Takeuchi T, Okamoto N, Watanabe T (1992b) The effect of dietary gelatinized rations at different dietary energy levels on growth and characteristics of blood in carp fingerlings. Bull Jpn Soc Sci Fish 58:945–951

    Article  Google Scholar 

  • Kayne F (1973) Pyruvate kinase, the enzymes, 3rd edn. Academic Press, New York, p 353

    Google Scholar 

  • Kumar S, Sahu NP, Pal AK, Choudhury D, Mukherjee SC (2006) Studies on digestibility and digestive enzyme activities in Labeo rohita (Hamilton) juveniles: effect of microbial α–amylase supplementation in non-gelatinized or gelatinized corn based diet at two protein level. Fish Physiol Biochem 32:209–220. doi:10.1007/s10695-006-9002-z

    Article  CAS  Google Scholar 

  • Kumar V, Sahu NP, Pal AK, Kumar S (2007) Immunomodulation of Labeo rohita juveniles due to dietary gelatinized and non-gelatinized starch. Fish Shellfish Immunol 23:341–353. doi:10.1016/j.fsi.2006.11.008

    Article  CAS  Google Scholar 

  • Kumar V, Sahu NP, Pal AK, Kumar S, Gupta SK (2008a) Gelatinized to non-gelatinized starch ratio in the diet of Labeo rohita: effect on digestive and metabolic response and on growth. J Anim Physiol Anim Nutr (Berl) 92:492–501. doi:10.1111/j.1439-0396.2007.00739.x

    Article  CAS  Google Scholar 

  • Kumar S, Sahu NP, Pal AK, Sagar V, Sinha AK, Baruah K (2008b) Modulation of key metabolic enzyme of Labeo rohita (Hamilton) juvenile: effect of dietary starch type, protein level and exogenous a-amylase in the diet. Fish Physiol Biochem. doi:10.1007/s10695-008-9213-6. (in press)

    Article  CAS  PubMed  Google Scholar 

  • Lee DJ, Putnam GB (1973) The response of rainbow trout to varying protein/energy ratios in a test diet. J Nutr 103:916–922

    Article  CAS  Google Scholar 

  • Marjorie AS (1964) In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol II. Academic Press Inc, New York, p 541

  • Mohapatra M, Sahu NP, Chaudhari A (2002) Utilization of gelatinized carbohydrate in diets in Labeo rohita fry. Aquacult Nutr 8:1–8. doi:10.1046/j.1365-2095.2002.00176.x

    Article  Google Scholar 

  • Ochoa S (1955) Malic dehydrogenase and ‘malic’ enzyme. In: Coloric SP, Kaplan N (eds) Methods of enzymology I. Academic Press, New York, pp 735–745

    Chapter  Google Scholar 

  • Peres MH, Oliva-Teles A (2002) Utilization of raw and gelatinized starch by European sea bass (Dicentrarchus labrax) juveniles. Aquaculture 205:287–299. doi:10.1016/S0044-8486(01)00682-2

    Article  Google Scholar 

  • Rosas C, Cuzon G, Gaxiola G, LePriol Y, Pascual C, Rossignyiol J, Contreras F, Sanchez A, Van Wormhoudt A (2001) Metabolism and growth of juveniles of L. vannamei: effect of salinity and dietary carbohydrates level. J Exp Mar Biol Ecol 259:1–22. doi:10.1016/S0022-0981(01)00222-2

    Article  CAS  Google Scholar 

  • Small BC, Soares JH Jr (1999) Effect of dietary carbohydrate on growth, glucose tolerance and liver composition of juveniles striped bass. N Am J Aquaculture 61:286–292. doi:10.1577/1548-8454(1999)061<0286:EODCOG>2.0.CO;2

    Article  Google Scholar 

  • Suárez MD, Sanz A, Bazoco J, García-Gallego M (2002) Metabolic effects of changes in the dietary protein: carbohydrate ratio in eel (Angilla anguilla) and trout (Oncorhynchus mykiss). Aquacult Int 10:143–156. doi:10.1023/A:1021371104839

    Article  Google Scholar 

  • Tranulis MA, Dregni O, Christophersen B, Krogdahl A, Borrebaek B (1996) A glucokinase-like enzyme in the liver of Atlantic salmon (Salmo salar). Comp Biochem Physiol 114B:35–39

    Article  CAS  Google Scholar 

  • Tung PH, Shiau SY (1993) Carbohydrate utilization versus body size in tilapia Oreochromis niloticus × O. aureus. Comp Biochem Physiol A 104:585–588. doi:10.1016/0300-9629(93)90468-J

    Article  Google Scholar 

  • Verma AK, Pal AK, Manush SM, Das T, Dalvi RS, Chandrachoodan PP, Ravi PM, Apte SK (2007) Persistent sub-lethal chlorine exposure elicits the temperature induced stress responses in Cyprinus carpio early fingerlings. Pestic Biochem Physiol 87:229–237. doi:10.1016/j.pestbp.2006.08.001

    Article  CAS  Google Scholar 

  • Walton MJ (1986) Metabolic effects of feeding a high protein/low carbohydrate diet as compared to a low protein/high carbohydrate diet to rainbow trout Salmo gairdneri. Fish Physiol Biochem 1:7–15. doi:10.1007/BF02309589

    Article  CAS  Google Scholar 

  • Walton MJ, Cowey CB (1982) Aspects of intermediary metabolism in salmonid fish. Comp Biochem Physiol 73B:59–79

    CAS  Google Scholar 

  • Wilson RP (1994) Utilization of dietary carbohydrate by fish. Aquaculture 124:67–80. doi:10.1016/0044-8486(94)90363-8

    Article  CAS  Google Scholar 

  • Wilson RP, Poe WE (1987) Apparent inability of channel catfish to utilize dietary mono- and disaccharides as energy sources. J Nutr 117:280–285

    Article  CAS  Google Scholar 

  • Wootton IDP (1964) Enzymes in blood. In: Churchill J, Churchill A (eds) Microanalysis in medical biochemistry, 4th edn. Churchill, London, UK, pp 101–107

    Google Scholar 

  • Wroblewski L, Ladue JS (1955) LDH activity in blood. Proc Soc Exp Biol Med 90:210–213

    Article  CAS  Google Scholar 

  • Yengkokpam S, Sahu NP, Pal AK, Mukherjee SC, Debnath D (2006) Gelatinized carbohydrates in the diet of Catla catla Fingerlings: effect of levels and sources on nutrient utilization, body composition and tissue enzyme activities. Asian-australas J Anim Sci 20:89–99

    Article  Google Scholar 

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Acknowledgments

The authors are grateful to the Director, Central Institute of Fisheries Education, Mumbai, for providing facilities for carrying out the work. The first author is grateful to the Central Institute of Fisheries Education, Mumbai, for awarding the institutional fellowship.

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Authors and Affiliations

  1. Department of Aquaculture System and Animal Nutrition in the Tropics and Subtropics (480b), University of Hohenheim, 70599, Stuttgart, Germany

    Vikas Kumar

  2. Division of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    N. P. Sahu, A. K. Pal & Shivendra Kumar

  3. Laboratory of Aquaculture and Artemia, Department of Animal Production, Ghent University, 9000, Ghent, Belgium

    Amit Kumar Sinha, Jayant Ranjan & Kartik Baruah

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  1. Vikas Kumar
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Correspondence to Kartik Baruah.

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Kumar, V., Sahu, N.P., Pal, A.K. et al. Modulation of key enzymes of glycolysis, gluconeogenesis, amino acid catabolism, and TCA cycle of the tropical freshwater fish Labeo rohita fed gelatinized and non-gelatinized starch diet. Fish Physiol Biochem 36, 491–499 (2010). https://doi.org/10.1007/s10695-009-9319-5

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