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Fish Physiology and Biochemistry

, Volume 36, Issue 3, pp 491–499 | Cite as

Modulation of key enzymes of glycolysis, gluconeogenesis, amino acid catabolism, and TCA cycle of the tropical freshwater fish Labeo rohita fed gelatinized and non-gelatinized starch diet

  • Vikas Kumar
  • N. P. Sahu
  • A. K. Pal
  • Shivendra Kumar
  • Amit Kumar Sinha
  • Jayant Ranjan
  • Kartik Baruah
Article

Abstract

A 60-day experiment was conducted to study the effect of dietary gelatinized (G) and non-gelatinized (NG) starch on the key metabolic enzymes of glycolysis (hexokinase, glucokinase, pyruvate kinase, and lactate dehydrogenase), gluconeogenesis (glucose-6 phosphatase and fructose-1,6 bisphosphatase), protein metabolism (aspartate amino transferase and alanine amino transferase), and TCA cycle (malate dehydrogenase) in Labeo rohita juveniles. In the analysis, 234 juveniles (2.53 ± 0.04 g) were randomly distributed into six treatment groups each with three replicates. Six semi-purified diets containing NG and G cornstarch, each at six levels of inclusion (0, 20, 40, 60, 80, and 100) were prepared viz., T1 (100% NG, 0% G starch), T2 (80% NG, 20% G starch), T3 (60% NG, 40% G starch), T4 (40% NG, 60% G starch), T5 (20% NG, 80% G starch), and T6 (0% NG, 100% G starch). Dietary G:NG starch ratio had a significant (P < 0.05) effect on the glycolytic enzymes, the highest activities were observed in the T6 group and lowest in the T1 group. On the contrary, the gluconeogenic enzymes, the glucose-6-phosphatase and fructose-1,6 bisphosphatase activities in the organs, liver and kidney were recorded highest in the T1 group and lowest in the T6 group. The liver aspartate amino transferase activity showed an increasing trend with the decrease in the dietary G level. However, the muscle aspartate amino transferase activity was not significantly (P > 0.05) influenced by the type of dietary starch. The alanine amino transferase activity in both liver and muscle showed an increasing trend with the decrease in the dietary G level. The liver and muscle malate dehydrogenase activities were lowest in the T6 group and highest in the T1 group. Results suggest that NG (100%) starch diet significantly induced more the enzyme activities of amino acid metabolism, gluconeogenesis, and TCA cycle, whereas partial or total replacement of raw starch by gelatinized starch increased the glycolytic enzyme activity.

Keywords

Starch Labeo rohita Hexokinase Glucokinase Pyruvate kinase Lactate dehydrogenase Glucose-6 phosphatase Fructose-1,6 bisphosphatase Aspartate amino transferase Alanine amino transferase Malate dehydrogenase 

Notes

Acknowledgments

The authors are grateful to the Director, Central Institute of Fisheries Education, Mumbai, for providing facilities for carrying out the work. The first author is grateful to the Central Institute of Fisheries Education, Mumbai, for awarding the institutional fellowship.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Vikas Kumar
    • 1
  • N. P. Sahu
    • 2
  • A. K. Pal
    • 2
  • Shivendra Kumar
    • 2
  • Amit Kumar Sinha
    • 3
  • Jayant Ranjan
    • 3
  • Kartik Baruah
    • 3
  1. 1.Department of Aquaculture System and Animal Nutrition in the Tropics and Subtropics (480b)University of HohenheimStuttgartGermany
  2. 2.Division of Fish Nutrition and BiochemistryCentral Institute of Fisheries Education, VersovaMumbaiIndia
  3. 3.Laboratory of Aquaculture and Artemia, Department of Animal ProductionGhent UniversityGhentBelgium

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