Fish Physiology and Biochemistry

, Volume 36, Issue 3, pp 445–450 | Cite as

Effects of repeated crowding on the stress response and growth performance in Atlantic salmon (Salmo salar)

  • T. V. Basrur
  • R. Longland
  • R. J. Wilkinson


A 64-day growth experiment was conducted in which two groups of Atlantic salmon parr were grown under either control conditions or subjected to a weekly crowding stressor. Subjecting fish to the stressor resulted in a 7.7% reduction in wet weight after 29 days, which was maintained at 7.9% by day 64. This reduction in weight was reflected in a 44% reduction in specific growth rate and 38% increase in feed conversion ratio over the first 29 days of the experiment. Elevation in plasma cortisol was observed in crowded fish on days 1, 29 and 64. Similarly, on days 1 and 29 an increase in both plasma glucose and lactate was detected. On day 64, however, no differences in plasma glucose and lactate were observed, with the magnitude of the cortisol response also significantly reduced. Overall, the relatively moderate impact on growth performance and reduction in magnitude of measured stress parameters at the end of the experiment suggests possible habituation to the applied stressor.


Atlantic salmon Stress Growth Cortisol 



This study was supported by research funds provided by the University of Tasmania and was completed in partial fulfilment of the requirements for the degree of Maters of Applied Science in Aquaculture for T.V. Basrur. Thanks to Saltas Pty. Ltd. (Wayatinah, Tasmania) for the supply of fish for this study.


  1. ABARE (2008) Australian Fisheries Statistics 2007, Canberra, JuneGoogle Scholar
  2. Barton BA (2002) Stress in fish: a diversity of responses with particular reference to changes in circulating corticosteroids. Integr Comp Biol 42:517–525. doi: 10.1093/icb/42.3.517 CrossRefGoogle Scholar
  3. Barton BA, Iwama GK (1991) Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Dis 1:3–26. doi: 10.1016/0959-8030(91)90019-G CrossRefGoogle Scholar
  4. Barton BA, Schreck CB, Barton LD (1987) Effects of chronic cortisol administration and daily acute stress on growth, physiological conditions, and stress response in juvenile rainbow trout. Dis Aquat Organ 2:173–185. doi: 10.3354/dao002173 CrossRefGoogle Scholar
  5. Dyer AR, Upton Z, Stone D, Thomas PM, Soole KL, Higgs N, Quinn K, Carragher JF (2004) Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels. Gen Comp Endocrinol 135:268–275. doi: 10.1016/j.ygcen.2003.10.002 CrossRefGoogle Scholar
  6. Hoskonen P, Pirhonen J (2006) Effects of repeated handling, with or without anesthesia, on feed intake and growth in juvenile rainbow trout, Oncorhynchus mykiss (Walbaum). Aquac Res 37:409–415. doi: 10.1111/j.1365-2109.2005.01448.x CrossRefGoogle Scholar
  7. Hosoya S, Johnson SC, Iwama GK, Gamperl AK, Afonso LOB (2007) Changes in free and total plasma cortisol levels in juvenile haddock (Melanogrammus aeglefinus) exposed to long-term handling stress. Comp Biochem Physiol A 146:78–86. doi: 10.1016/j.cbpa.2006.09.003 CrossRefGoogle Scholar
  8. Jentoft S, Aastveit AH, Torjesen PA, Andersen O (2005) Effects of stress on growth, cortisol and glucose levels in non-domesticated Eurasian perch (Perca fluviatilis) and domesticated rainbow trout (Oncorhynchus mykiss). Comp Biochem Physiol A 141:353–358. doi: 10.1016/j.cbpb.2005.06.006 CrossRefGoogle Scholar
  9. Massou AM, Le Bail PY, Panfili J, Lae R, Baroiller JF, Mikolasek O, Fontenelle G, Auperin B (2004) Effects of confinement stress of variable duration on the growth and microincrement deposition in the otoliths of Oreochromis niloticus (cichlidae). J Fish Biol 65:1253–1269. doi: 10.1111/j.0022-1112.2004.00512.x CrossRefGoogle Scholar
  10. McCormick SD, Shrimpton JM, Carey JB, O′Dea MF, Sloan KE, Moriyama S, Bjornsson BT (1998) Repeated acute stress reduces growth rate of Atlantic salmon parr and alters plasma levels of growth hormone, insulin-like growth factor I and cortisol. Aquaculture 168:221–235. doi: 10.1016/S0044-8486(98)00351-2 CrossRefGoogle Scholar
  11. Ovenden JR, Bywater R, White RWG (1993) Mitochondiral DNA sequence variation in Atlantic salmon (Salmo salar), brown trout (S. trutta), and rainbow trout (Oncorhynchus mykiss) and the brook trout (Salvelinus fontinalis) from Tasmania, Australia. Aquaculture 114:217–227. doi: 10.1016/0044-8486(93)90297-C CrossRefGoogle Scholar
  12. Pankhurst NW, Sharples DF (1992) Effects of capture and confinement on plasma cortisol concentrations in the Snapper, Pagrus auratus. Aust J Mar Freshwater Res 43:345–356. doi: 10.1071/MF9920345 CrossRefGoogle Scholar
  13. Pickering AD (1990) Stress and the suppression of somatic growth in teleosts fish. In: Prasad MRN (ed) Progress in comparative endocrinology. Wiley, New York, pp 112–134Google Scholar
  14. Poli BM, Parisi G, Scappini G, Zampacavallo G (2005) Fish welfare and quality as affected by pre-slaughter and slaughter managements. Aquac Int 13:29–49. doi: 10.1007/s10499-004-9035-1 CrossRefGoogle Scholar
  15. Randall DJ, Perry SF (1992) Catecholamines, Fish physiology. Academic Press, San Diego, pp 255–300Google Scholar
  16. Seyle H (1950) Stress and the general adaptation syndrome. BMJ 1:1383–1392CrossRefGoogle Scholar
  17. Strand A, Magnhagen C, Alanara A (2007) Effects of repeated disturbances on feed intake, growth rates and energy expenditures of juvenile perch, Perca fluvatilis. Aquaculture 265:163–168. doi: 10.1016/j.aquaculture.2007.01.030 CrossRefGoogle Scholar
  18. Wedemeyer GA, Barton BA, Mcleay DJ (1990) Stress and acclimation. In: Scherck CB, Moyle PB (eds) Methods for fish biology. American Fisheries Society, Maryland, pp 451–489Google Scholar
  19. Wilkinson RJ, Porter M, Woolcott H, Longland R, Carragher JF (2006) Effects of aquaculture related stressors and nutritional restriction on circulating growth factors (GH, IGF-I and IGF-II) in Atlantic salmon and rainbow trout. Comp Biochem Physiol A 145:214–224CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.School of Aquaculture, Tasmanian Aquaculture and Fisheries InstituteUniversity of TasmaniaLauncestonAustralia

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