Skip to main content
SpringerLink
Log in

Influence of high temperature on cadmium-induced skeletal deformities in juvenile mosquitofish (Gambusia affinis)

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

The aim of this study was to assess the effect of high temperature on cadmium (Cd)-induced skeletal deformities in juvenile Mosquitofish, Gambusia affinis. For this purpose, 188 juveniles (1 day old) were equally divided into the control group, which was maintained in Cd-free water at 24°C, and three treated groups exposed either to Cd (0.4 mg/l as Cd Cl2) at 24°C, to high temperature (32°C), or to Cd at 32°C for 30 days. The results showed that Cd exposure at 24°C significantly increased the Cd accumulation (P < 0.0001) in the whole tissues of juveniles as well as the incidence of skeletal deformities (P < 0.01) compared with control animals. Exposure to high temperature also led to a significant increase in the incidence of skeletal deformities (P < 0.01) with respect to the control group. Interestingly, our results showed that the combined exposure to Cd and high temperature led to a more significant increase in Cd accumulation and in the frequency of spinal deformities than exposure to Cd or high temperature alone. These results confirm that temperature increases Cd toxicity and needs to be taken into account for the accurate prediction and assessment of Cd-induced spinal deformities in fish.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Afonso JM, Montero D, Robaina L, Astorga N, Izquierdo MS, Gines R (2000) Association of a lordosis-scoliosis-kyphosis deformity in gilthead sea bream (Sparus aurata) with family structure. Fish Physiol Biochem 22:159–163. doi:10.1023/A:1007811702624

    Article  CAS  Google Scholar 

  • Annabi A, Messaoudi I, Kerkeni A, Saïd K (2008) Comparative study of the sensitivity to cadmium of two populations of Gambusia affinis from two different sites. Environ Monit Assess. doi:10.1007/s10661-008-0448-6

    Article  CAS  PubMed  Google Scholar 

  • Bengtsson BE, Larsson A (1986) Vertebral deformities and physiological effects in fourhorn sculpin (Myoxocephalus quadricornis) after long-term exposure to a simulated heavy metal containing effluent. Aquat Toxicol 9:215–229. doi:10.1016/0166-445X(86)90010-X

    Article  CAS  Google Scholar 

  • Bengtsson A, Bengtsson BE, Lithner G (1985) Vertebral defects in fourhorn sculpin, Myoxocephalus quadricornis L, exposed to heavy metal pollution in the Gulf of Bothnia. J Fish Biol 33:517–529. doi:10.1111/j.1095-8649.1988.tb05496.x

    Article  Google Scholar 

  • Blumenthal N, Cosma V, Skyler D, Legeros J, Walters M (1995) The effect of cadmium on the formation and properties of hydroxyapatite in vitro and its relation to cadmium toxicity in the skeletal system. Calcif Tissue 56:316–322. doi:10.1007/BF00318053

    Article  CAS  Google Scholar 

  • Boglione C, Gagliardi F, Scardi M, Cataudella S (2001) Skeletal descriptors and quality assessment in larvae and post-larvae of wild-caught and hatchery-reared gilthead sea bream (Sparus aurata L. 1758). Aquaculture 192:1–22. doi:10.1016/S0044-8486(00)00446-4

    Article  Google Scholar 

  • Buhringer H, Sperling K, Wunder W (1990) Spinal shortening (osteosclerosis) in spawners of the rainbow trout. Arch Fischereiwiss 40:205–228

    Google Scholar 

  • Cahu C, Zambonino Infante J, Takeuchib T (2003) Nutritional components affecting skeletal development in fish larvae. Aquaculture 227:245–258. doi:10.1016/S0044-8486(03)00507-6

    Article  CAS  Google Scholar 

  • Campinho MA, Moutou KA, Power DM (2004) Temperature sensitivity of skeletal ontogeny in Oreochromis mossambicus. J Fish Biol 65:1003–1025. doi:10.1111/j.0022-1112.2004.00505.x

    Article  Google Scholar 

  • Cheng SH, Wai AWK, So CH, Wu RSS (2000) Cellular and molecular basis of cadmium-induced deformities in zebrafish embryos. Environ Toxicol Chem 19:3024–3031. doi:10.1897/1551-5028(2000)019<3024:CAMBOC>2.0.CO;2

    Article  CAS  Google Scholar 

  • Divanach P, Papandroulakis N, Anastasiadis P, Koumoundouros G, Kentouri M (1997) Effect of water currents on the development of skeletal deformities in sea bass (Dicentrarchus labrax L.) with functional swimbladder during postlarval and nursery phase. Aquaculture 156:145–155. doi:10.1016/S0044-8486(97)00072-0

    Article  Google Scholar 

  • Eisler R (1971) Cadmium poisoning in Fundulus hetroclitus (Pisces: Cyprinodontidae) and other organisms. J Fish Res Bd Can 28:1225–1234

    Article  CAS  Google Scholar 

  • Faustino M, Power D (1998) Development of osteological structures in the sea bream: vertebral column and caudal fin complex. J Fish Biol 52:11–22. doi:10.1111/j.1095-8649.1998.tb01548.x

    Article  Google Scholar 

  • Hallare AV, Schirling M, Luckenbach T, Köhler HR, Triebskorn R (2005) Combined effects of temperature and cadmium on developmental parameters and biomarker responses in zebrafish (Danio rerio) embryos. J Therm Biol 30:7–17. doi:10.1016/j.jtherbio.2004.06.002

    Article  CAS  Google Scholar 

  • Hubbs C (1959) High incidence of vertebral deformities in two natural populations of fishes inhabiting warm springs. Ecology 40:154–155. doi:10.2307/1929941

    Article  Google Scholar 

  • Kessabi K, Kerkeni A, Saïd K, Messaoudi I (2008) Involvement of cadmium bioaccumulation in spinal deformities occurrence in natural population of Mediterranean Killifish. Biol Trace Elem Res. doi:10.1007/s12011-008-8255-z

    Article  CAS  PubMed  Google Scholar 

  • Koumoundouros G, Divanach P, Anezaki L, Kentouri M (2001) Temperature-induced ontogenetic plasticity in sea bass (Dicentrarchus labrax). Mar Biol (Berl) 139:817–830. doi:10.1007/s002270100635

    Article  Google Scholar 

  • Kranenbarg S, Waarsing JH, Muller M, Weinans H, van Leeuwen JL (2005) Lordotic vertebrae in sea bass (Dicentrarchus labrax L) are adapted to increased loads. J Biomech 38:1239–1246. doi:10.1016/j.jbiomech.2004.06.011

    Article  Google Scholar 

  • Leung KM, Taylor AC, Furness R (2000) Temperature dependent physiological responses of the dogwhelk Nucella lapillus to cadmium exposure. J Mar Biol Assoc U K 80:647–660. doi:10.1017/S0025315400002472

    Article  CAS  Google Scholar 

  • Messaoudi I, Deli T, Kessabi K, Barhoumi S, Kerkeni A, Saïd K (2008a) Association of spinal deformities with heavy metal bioaccumulation in natural populations of grass goby, Zosterisessor ophiocephalus Pallas, 1811 from the Gulf of Gabès (Tunisia). Environ Monit Assess. doi:10.1007/s10661-008-0504-2

    Article  CAS  PubMed  Google Scholar 

  • Messaoudi I, Kessabi K, Kacem A, Saïd K (2008b) Incidence of spinal deformities in natural populations of Aphanius fasciatus Nardo, 1827 from the Gulf of Gabès, Tunisia. Afr J Ecol. doi:10.1111/j.1365-2028.2008.00972.x

    Article  Google Scholar 

  • Mitton JB (1971) Meristic abnormalities in Fundulus heteroclitus. In studies on the effects of a steam-electric generating plant on the marine environment at Northport, New York. Tech Rep Mar Sci 9:105–109

    Google Scholar 

  • Moss ML (1961) Studies of the acellular bone of teleost fish. I. Morphological and systematic variations. Acta Anat (Basel) 46:343–362. doi:10.1159/000141794

    Article  CAS  Google Scholar 

  • Moss ML (1965) Studies of the acellular bone of teleost fish. V. Histology and mineral homeostasis of fresh-water species. Acta Anat (Basel) 60:262–276. doi:10.1159/000142652

    Article  CAS  Google Scholar 

  • Muramoto S (1981) Vertebral column damage and decrease of calcium concentration in fish exposed experimentally to cadmium. Environ Pollut Ser 24:125–133. doi:10.1016/0143-1471(81)90074-X

    Article  CAS  Google Scholar 

  • Park EH, Kim DS (1984) A procedure for staining cartilage and bone of whole vertebrate larvae while rendering all other tissues transparent. Stain Technol 59:269–272

    Article  CAS  Google Scholar 

  • Rathore SR, Khangarot BS (2002) Effects of temperature on the sensitivity of Sludge worm Tubifex tubifex Müller to selected heavy metals. Ecotoxicol Environ Saf 53:27–36. doi:10.1006/eesa.2001.2100

    Article  CAS  Google Scholar 

  • Roy PK, Lall SP (2007) Vitamin K deficiency inhibits mineralization and enhances deformity in vertebrae of haddock (Melanogrammus aeglefinus L.). Comparative Biochem Physiol. Part B 148:174–183

    Article  Google Scholar 

  • Sfakianakis DG, Koumoundouro G, Divanach P, Kentouri M (2004) Osteological development of the vertebral column and of the fins in Pagellus erythrinus (L. 1758). Temperature effect on the developmental plasticity and morpho-anatomical abnormalities. Aquaculture 232:407–424. doi:10.1016/j.aquaculture.2003.08.014

    Article  Google Scholar 

  • Suzuki Y, Morita I, Ishizaki Y, Yamane Y, Murota S (1989) Cadmium stimulates prostaglandin E2 synthesis in osteoblast-like cells. Biochim Biophys Acta 1012:135–139. doi:10.1016/0167-4889(89)90086-4

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. UR 09/30: Génétique, Biodiversité et Valorisation des Bioressources, Institut de Biotechnologie, 5000, Monastir, Tunisia

    Asma Sassi, Ali Annabi, Kaouthar Kessabi, Khaled Saïd & Imed Messaoudi

  2. Eléments Trace, Radicaux Libres, Systèmes Antioxydants et Pathologies Humaines et Environnement, Faculté de Médecine, 5000, Monastir, Tunisia

    Abdelhamid Kerkeni

Authors
  1. Asma Sassi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ali Annabi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kaouthar Kessabi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Abdelhamid Kerkeni
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Khaled Saïd
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Imed Messaoudi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Imed Messaoudi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sassi, A., Annabi, A., Kessabi, K. et al. Influence of high temperature on cadmium-induced skeletal deformities in juvenile mosquitofish (Gambusia affinis). Fish Physiol Biochem 36, 403–409 (2010). https://doi.org/10.1007/s10695-009-9307-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10695-009-9307-9

Keywords

Search

Navigation