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Fish Physiology and Biochemistry

, Volume 35, Issue 3, pp 467–478 | Cite as

Effect of a probiotic bacterium Bacillus circulans PB7 in the formulated diets: on growth, nutritional quality and immunity of Catla catla (Ham.)

  • Partha Bandyopadhyay
  • Pradeep K. Das Mohapatra
Article

Abstract

Bacillus circulans PB7, isolated from the intestine of Catla catla, was evaluated for use as a probiotic supplement in the feeds for the fingerlings of Catla catla. The effect of supplement on the growth performance, feed utilization efficiency, and immune response was evaluated. Catla fingerlings (ave. wt. 6.48 ± 0.43 g) were fed diets supplemented with 2 × 104 (feed C1), 2 × 105 (feed C2), and 2 × 106 (feed C3) B. circulans PB 7 cells per 100 g feed for 60 days at 5% of the body weight per day in two equal instalments in triplicate treatments. The control feed (CC) was not supplemented with the B. circulans. All the feeds were isocaloric and isonitrogenous. Fish fed with feed C2 displayed better growth, significantly (P ≤ 0.05) highest RNA/DNA ratio, a lower feed conversion ratio (FCR), and a higher protein efficiency ratio (PER) than the other experimental diets. Highest carcass protein and lipid was also observed in the fish fed C2 feed compared to the others. Significantly (P ≤ 0.05), highest protease was recorded in fish fed feed C2 (47.9 ± 0.016) and lowest in fish fed feed C3 (32.10 ± 0.009), where α-amylase activity did not differ significantly (P ≤ 0.05) beyond the lowest inclusion level. ALP, ACP, GOT, and GPT in the liver of Catla catla were the highest (P ≤ 0.05) in fish fed C2 feed. The highest TSP, albumin, and globulin was observed in fish treated with C2 feed after 60 days feeding trial, but the lowest glucose level was observed in the same treatment. After the feeding trial, the non-specific immunity levels and disease resistance of fish were also studied. Phagocytic ratio, phagocytic index, and leucocrit value were the highest in fish fed feed C2. After the feeding trial, the fish were challenged for 10 days by bath exposure to Aeromonas hydrophila (AH1) (105c.f.u. ml−1 for 1 h, and, after 7 days, 107c.f.u. ml−1 for 1 h). Highest survival percentage was observed in fish fed with feed C2 compared with only 6.66% in the controls, which indicated the effectiveness of B. circulans PB 7 in reducing disease caused by A. hydrophila.

Keywords

Bacillus circulans Probiotics Catla catla 

Notes

Acknowledgements

The first author is highly thankful to Indian Council of Agricultural Research, New Delhi, India for financial assistance. The author is also thankful to Head of the Department, Department of Zoology and Hon’ble Vice-Chancellor, Vidyasagar University for providing necessary facilities.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Partha Bandyopadhyay
    • 1
    • 2
  • Pradeep K. Das Mohapatra
    • 3
  1. 1.Aquaculture Research Unit, Department of ZoologyVidyasagar UniversityMidnaporeIndia
  2. 2.Biostadt India LimitedMumbaiIndia
  3. 3.Department of MicrobiologyVidyasagar UniversityMidnaporeIndia

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