Leukocyte response and phagocytic activity in Nile tilapia experimentally infected with Enterococcus sp.
This study evaluated the total and differential leukocyte counting and the phagocytic activity in Nile tilapia Oreochromis niloticus experimentally injected with Enterococcus sp. in the swim bladder. Fish were distributed in four treatments in triplicates of non-injected fish, fish injected with 1 ml of sterile saline solution 0.65%, and fish injected with 1 × 103 and 1 × 106 colony-forming units (CFU) of Enterococcus diluted in 1 ml sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected for white blood cell (WBC) counts, differential counting of WBC, and phagocytic activity of blood leukocytes. The increased numbers of WBC and lymphocytes were followed by decreased number of monocyte after infection. The percentages of phagocytic activities in the blood were 55.3 and 55.9%, respectively, in tilapia injected with 1 × 103 and 1 × 106 CFU/ml.
KeywordsInfection Leukocyte Phagocytosis Tilapia
The authors thank “National Council of Scientific and Technologic Development/CNPq-300281/04-8) for the grant to M.L. Martins, Microbiology Laboratory of Marine Shrimp Laboratory, UFSC, SC, for aid in bacteria isolation and Roberto Hoppe (Fundação 25 de julho, Joinville, SC) for fish donation.
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