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Leukocyte response and phagocytic activity in Nile tilapia experimentally infected with Enterococcus sp.

  • Maurício L. Martins
  • Felipe N. Vieira
  • Gabriela T. Jerônimo
  • José L. P. Mouriño
  • Geovana Dotta
  • Gisele M. Speck
  • Adolfo J. M. Bezerra
  • Fabíola S. Pedrotti
  • Celso C. Buglione-Neto
  • Gilberto PereiraJr
Article

Abstract

This study evaluated the total and differential leukocyte counting and the phagocytic activity in Nile tilapia Oreochromis niloticus experimentally injected with Enterococcus sp. in the swim bladder. Fish were distributed in four treatments in triplicates of non-injected fish, fish injected with 1 ml of sterile saline solution 0.65%, and fish injected with 1 × 103 and 1 × 106 colony-forming units (CFU) of Enterococcus diluted in 1 ml sterile saline. Twenty-four hours after injection, the fish were anesthetized and the blood collected for white blood cell (WBC) counts, differential counting of WBC, and phagocytic activity of blood leukocytes. The increased numbers of WBC and lymphocytes were followed by decreased number of monocyte after infection. The percentages of phagocytic activities in the blood were 55.3 and 55.9%, respectively, in tilapia injected with 1 × 103 and 1 × 106 CFU/ml.

Keywords

Infection Leukocyte Phagocytosis Tilapia 

Notes

Acknowledgments

The authors thank “National Council of Scientific and Technologic Development/CNPq-300281/04-8) for the grant to M.L. Martins, Microbiology Laboratory of Marine Shrimp Laboratory, UFSC, SC, for aid in bacteria isolation and Roberto Hoppe (Fundação 25 de julho, Joinville, SC) for fish donation.

References

  1. Anderson DP (1990) Immunological indicators: effects of environmental stress on immune protection and disease outbreaks. American fisheries society symposium, Bethesda, MD, vol 8, pp 38–50Google Scholar
  2. Balfry SK, Shariff M, Iwama GK (1997) Strain differences in non-specific immunity of tilapia Oreochromis niloticus following challenge with Vibrio parahaemolyticus. Dis Aquat Organ 30:77–80. doi: 10.3354/dao030077 CrossRefGoogle Scholar
  3. Cai W-Q, Li S-F, Ma J-Y (2004) Diseases resistance of Nile tilapia (Oreochromis niloticus), blue tilapia (Oreochromis aureus) and their hybrid (female Nile tilapia x male blue tilapia) to Aeromonas sobria. Aquaculture 229:79–87. doi: 10.1016/S0044-8486(03)00357-0 CrossRefGoogle Scholar
  4. Cavichiolo F, Vargas L, Ribeiro RP, Moreira HLM, Leonardo JM (2002) Níveis de suplementação de vitamina C na ração sobre a ocorrência de ectoparasitas, sobrevivência e biomassa em alevinos de tilápia do Nilo (Oreochromis niloticus L.). Acta Sci 24:957–964Google Scholar
  5. Garcia F, Pilarski F, Onaka EM, Moraes FR, Martins ML (2007) Hematology of Piaractus mesopotamicus fed diets supplemented with vitamins C and E, challenged by Aeromonas hydrophila. Aquaculture 271:39–46. doi: 10.1016/j.aquaculture.2007.06.021 CrossRefGoogle Scholar
  6. Ghiraldelli L, Martins ML, Yamashita MM, Jerônimo GT (2006) Ectoparasites influence on the haematological parameters of Nile tilapia and carp cultured in the State of Santa Catarina, South Brazil. J Fish Aquat Sci 1:270–276CrossRefGoogle Scholar
  7. Haney DC, Hursh DA, Mix MC, Winton JR (1992) Physiological and haematological changes in chum salmon artificially infected with erythrocytic necrosis virus. J Aquat Anim Health 4:48–57. doi:10.1577/1548-8667(1992)004<0048:PAHCIC>2.3.CO;2CrossRefGoogle Scholar
  8. Harikrishnan R, Nisha-Rani M, Balasundaram C (2003) Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture 221:41–50. doi: 10.1016/S0044-8486(03)00023-1 CrossRefGoogle Scholar
  9. Hrubec TC, Cardinale JL, Smith SA (2000) Hematology and plasma chemistry reference intervals for cultured tilapia (Oreochromis hybrid). Vet Clin Pathol 29:7–12. doi: 10.1111/j.1939-165X.2000.tb00389.x PubMedCrossRefGoogle Scholar
  10. Lamas J, Santos Y, Bruno DW, Toranzo AE, Anadon R (1994) Non-specific cellular responses of rainbow trout Vibrio anguillarum and its extracellular products. J Fish Biol 45(5):839–854. doi: 10.1111/j.1095-8649.1994.tb00949.x CrossRefGoogle Scholar
  11. Martins ML, Nomura DT, Myiazaki DMY, Pilarsky F, Ribeiro K, Castro MP et al (2004) Physiological and haematological response of Oreochromis niloticus (Osteichthyes: Cichlidae) exposed to single and consecutive stress of capture. Acta Sci Anim Sci 26:449–456Google Scholar
  12. Martins ML, Mouriño JLP, Amaral GV, Vieira FN, Dotta G, Bezerra AJM et al (2008) Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. Braz J Biol 68(3):631–637CrossRefGoogle Scholar
  13. Matushima ER, Mariano M (1996) Kinetics of the inflammatory reaction induced by carrageenin in the swim bladder of Oreochromis niloticus (Nile tilapia). Braz J Vet Res Anim Sci 33:5–10Google Scholar
  14. Moraes FR, Martins ML (2004) Favourable conditions and principal teleostean diseases in intensive fish farming. In: Cyrino JEP, Urbinati EC, Fracalossi DM, Castagnolli N (eds) Especial topics in tropical intensive freshwater fish farming. TecArt, Sao Paulo, pp 343–383Google Scholar
  15. Pathiratne A, Rajapakshe W (1998) Hematological changes associated with epizootic ulcerative syndrome in the Asian cichlid fish, Etroplus suratensis. Asian Fish Sci 11:203–211Google Scholar
  16. Ranzani-Paiva MJT, Ishikawa CM, Eiras AC, Silveira VR (2004) Effects of an experimental challenge with Mycobacterium marinum on the blood parameters of Nile Tilapia, Oreochromis niloticus (Linnaeus, 1757). Braz Arch Biol Technol 47(6):945–953. doi: 10.1590/S1516-89132004000600014 CrossRefGoogle Scholar
  17. Ranzani-Paiva MJT, Felizardo NN, Luque JL (2005) Parasitological and haematological analysis of Nile tilapia Oreochromis niloticus Linnaeus, 1757 from Guarapiranga reservoir, São Paulo State, Brazil. Acta Sci 27(3):231–237Google Scholar
  18. Rosenfeld G (1947) Corante pancrômico para hematologia e citologia clínica Nova combinação dos componentes do May-Grünwald e do Giemsa num só corante de emprego rápido. Mem Inst Butantan 20:329–334Google Scholar
  19. Ruane NM, Nolan DT, Rotlant J, Costello EJ, Weendellar Bonga SE (2000) Experimental exposure of rainbow trout Oncorhynchus mykiss (Walbaum) to the infective stages of the sea louse Lepeophtheirus salmonis (Kroyer) influences the physiological response to an acute stressor. Fish Shellfish Immunol 10:451–463. doi: 10.1006/fsim.1999.0260 PubMedCrossRefGoogle Scholar
  20. Siwicki A, Studnicka M (1987) The phagocytic ability of neutrophils and serum lysozyme activity in experimentally infected carp, Cyprinus carpio L. J Fish Biol 31(Suppl A):57–60CrossRefGoogle Scholar
  21. Tavares-Dias M, Moraes FR, Martins ML, Santana AE (2002) Haematological changes in Oreochromis niloticus (Osteichthyes: Cichlidae) with gill ichthyophthiriasis and saprolegniosis. Bol Inst Pesca 28:1–9Google Scholar
  22. Toranzo AE, Devesa S, Romalde JL, Lamas J, Riaza A, Leiro J et al (1995) Efficacy of intraperitoneal and immersion vaccination against Enterococcus sp infection in turbot. Aquaculture 134:17–27. doi: 10.1016/0044-8486(95)00038-4 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Maurício L. Martins
    • 1
  • Felipe N. Vieira
    • 2
    • 3
  • Gabriela T. Jerônimo
    • 2
  • José L. P. Mouriño
    • 3
  • Geovana Dotta
    • 2
  • Gisele M. Speck
    • 2
  • Adolfo J. M. Bezerra
    • 3
  • Fabíola S. Pedrotti
    • 3
  • Celso C. Buglione-Neto
    • 3
  • Gilberto PereiraJr
    • 2
  1. 1.Laboratory of Diagnosis and Pathology in Aquaculture, Aquaculture DepartmentFederal University of Santa Catarina (UFSC)FlorianópolisBrazil
  2. 2.Aquaculture DepartmentFederal University of Santa Catarina (CCA, UFSC)FlorianópolisBrazil
  3. 3.Marine Shrimp Laboratory, Aquaculture DepartmentCCA, UFSCFlorianópolisBrazil

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