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Modulation of key metabolic enzyme of Labeo rohita (Hamilton) juvenile: effect of dietary starch type, protein level and exogenous α-amylase in the diet

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Abstract

A 60-day feeding trial was conducted to delineate the effect of both gelatinized (G) and non-gelatinized (NG) corn with or without supplementation of exogenous α-amylase, either at optimum (35%) or sub-optimum (27%) protein levels, on blood glucose, and the key metabolic enzymes of glycolysis (hexokinase, HK), gluconeogenesis (glucose-6 phosphatase, G6Pase and fructose-1,6 bisphosphatase, FBPase), lipogenesis (glucose-6 phaosphate dehydrogenase, G6PD) and amino acid metabolism (alanine amino transfersae, ALT and aspartate amino transferase, AST) in Labeo rohita. Three hundred and sixty juveniles (average weight 10 ± 0.15 g) were randomly distributed into 12 treatment groups with each of two replicates. Twelve semi-purified diets containing either 35 or 27% crude protein were prepared by including G or NG corn as carbohydrate source with different levels of microbial α-amylase (0, 50, 100 and 150 mg kg−1). The G corn fed groups showed significantly higher (P < 0.05) blood glucose and G6PD activity, whereas G6Pase, FBPase, ALT and AST activity in liver was higher in the NG corn fed group. Dietary corn type, α-amylase level in diet or their interaction had no significant effect (P > 0.05) on liver HK activity, but the optimum crude protein (35%) fed group showed higher HK activity than their low protein counterparts. The sub-optimum crude protein (27%) fed group showed significantly higher (P < 0.05) G6PD activity than the optimum protein fed group, whereas the reverse trend was observed for HK, G6Pase, FBPase, ALT and AST activity. Addition of 50 mg α-amylase kg−1 feed showed increased blood glucose and G6PD activity of the NG corn fed group, whereas the reverse trend was found for G6Pase, FBPase, ALT and AST activity in liver, which was similar to that of the G or NG corn supplemented with 100/150 mg α-amylase kg−1 feed. Data on enzyme activities suggest that NG corn in the diet significantly induced more gluconeogenic and amino acid metabolic enzyme activity, whereas G corn induced increased lipogenic enzyme activity. Increased amino acid catabolic enzyme (ALT and AST) activity was observed either at optimum protein (35%) irrespective of corn type or NG corn without supplementation of α-amylase irrespective of protein level in the diet.

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Acknowledgements

The authors are grateful to the Director, Central Institute of Fisheries Education, Mumbai for providing facilities for carrying out the work. The first author is grateful to Central Institute of Fisheries Education, Mumbai for awarding the institutional fellowship.

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  1. Department of Fish Nutrition and Biochemistry, Central Institute of Fisheries Education, Versova, Mumbai, 400061, India

    Shivendra Kumar, N. P. Sahu, A. K. Pal, Vidya Sagar & Kartik Baruah

  2. Department of Animal Production, Ghent University, Ghent, Belgium

    Amit Kumar Sinha & Kartik Baruah

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  1. Shivendra Kumar
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  2. N. P. Sahu
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Correspondence to Shivendra Kumar.

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Kumar, S., Sahu, N.P., Pal, A.K. et al. Modulation of key metabolic enzyme of Labeo rohita (Hamilton) juvenile: effect of dietary starch type, protein level and exogenous α-amylase in the diet. Fish Physiol Biochem 35, 301–315 (2009). https://doi.org/10.1007/s10695-008-9213-6

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