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Thyroxine-induced alterations in the testis and seminal vesicles of air-breathing catfish, Clarias gariepinus

  • T. N. Jacob
  • J. P. Pandey
  • K. Raghuveer
  • G. Sreenivasulu
  • A. D. Gupta
  • M. Yoshikuni
  • A. Jagota
  • B. Senthilkumaran
Research Article

Abstract

Effect of experimentally induced thyroxine overdose on the testis and seminal vesicles was studied in the air-breathing catfish, Clarias gariepinus during the preparatory and the pre-spawning phase. The present study revealed a marked reduction in testosterone level in serum, testis and seminal vesicles (SV). Histological examination showed a considerable reduction in the number of spermatozoa/spermatids in the seminiferous tubular lumen as well as depletion of fluid in the loculi of SV. SDS-PAGE analysis of SV fluid proteins demonstrated a significant decrease in the level of a ~27 kDa protein in thyroxine treated fishes. Evidences are presented here to indicate that thyroid hormone plays a role in regulating testis and SV function in catfish.

Keywords

Clarias gariepinus Seminal vesicles Spermatogenesis Testis Thyroxine 

References

  1. Mansour N, Lahnsteiner F, Patzner RA (2004) Seminal vesicle secretion of African catfish, its composition its behavior in water and saline solutions and its influence on gamete fertilizability. J Exp Biol 301:745–755Google Scholar
  2. Maran RRM (2003) Thyroid hormones: their role in testicular steroidogenesis. Arch Androl 49:375–388CrossRefPubMedGoogle Scholar
  3. Matta SL, Vilela DA, Godinho HP, Franca LR (2002) The goitrogen 6-n-propyl-2-thiouracil (PTU) given during testis development increases Sertoli and germ cell numbers per cyst in fish: the tilapia (Oreochromis niloticus) model. Endocrinology 143:970–978CrossRefPubMedGoogle Scholar
  4. Misra M, Pandey K (1985) Effect of thiourea on the testicular steroidogenesis and secondary sexual characters of a tropical freshwater fish, Mystus vittatus (Bloch). Ann Endocrinol (Paris) 46:421–425Google Scholar
  5. Plate EM, Adams BA, Allison WT, Martens G, Hawryshyn CW, Eales JG (2002) The effects of thyroxine or a GnRH analogue on thyroid hormone deiodination in the olfactory epithelium and retina of rainbow trout, Oncorhynchus mykiss, and sockeye salmon, Oncorhynchus nerka. Gen Comp Endocrinol 127:59–65CrossRefPubMedGoogle Scholar
  6. Senthilkumaran B, Joy KP (1993) Annual cyclic, and castration and cytroterone acetate-induced, changes in sialic acid content of the seminal vesicle of the catfish, Heteropnustes fossilis (Bloch). Fish Physiol Biochem 10:425–430CrossRefGoogle Scholar
  7. Timmermans LPM, Chmilevsky DA, Komen H, Schipper H (1997) Precocious onset of spermatogenesis in juvenile Carp (Cyprinus carpio L. Teleostei) following treatment with low doses of L-thyroxine. Eur J Morphol 35:344–353CrossRefPubMedGoogle Scholar
  8. van den Hurk R, Resink JW, Peute J (1987) The seminal vesicle of the African catfish, Clarias gariepinus. A histological, biochemical, enzyme-histochemical, ultrastructutal and physiological study. Cell Tissue Res 247:573–582CrossRefPubMedGoogle Scholar
  9. Viveiros ATM, Eding EH, Komen J (2001) Effect of 17 α-methyltestosterone on seminal vesicle development and semen release response in the African catfish, Clarias gariepinus. Reproduction 122:817–827CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • T. N. Jacob
    • 1
  • J. P. Pandey
    • 1
  • K. Raghuveer
    • 1
  • G. Sreenivasulu
    • 1
  • A. D. Gupta
    • 1
  • M. Yoshikuni
    • 2
  • A. Jagota
    • 1
  • B. Senthilkumaran
    • 1
  1. 1.Department of Animal Sciences, School of Life SciencesUniversity of HyderabadHyderabadIndia
  2. 2.Laboratory of Reproductive BiologyNational Institute for Basic BiologyOkazakiJapan

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